DOI: 10.1111/j.1365-2958.1995.tb02282.x. Mutations in the chemotactic response regulator, CheY, that confer resistance to the phosphatase activity of CheZ

Mutations in the chemotactic response regulator, CheY, that confer resistance to the phosphatase activity of CheZ

10.1111/j.1365-2958.1995.tb02282.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

SummaryCheY, a small cytoplasmic response regulator, plays an essential role in the chemotaxis pathway. The concentration of phospho‐CheY is thought to determine the swimming behaviour of the cell: high levels of phospho‐CheY cause bacteria to rotate their flagella clockwise and tumble, whereas low levels of the phos‐phorylated form of the protein allow counter‐ciockwise rotation of the flagella and smooth swimming. The phosphorylation state of CheY in vivo is determined by the activity of the phosphoryl donor CheA, and by the antagonistic effect of dephosphorylation of phospho‐CheY. The dephosphorylation rate is controlled by the intrinsic autohydrolytic activity of phospho‐CheY and by the CheZ protein, which accelerates dephosphorylation. We have analysed the effect of CheZ on the dephosphorylation rates of several mutant CheY proteins. Two point mutations were identified which were 50‐fold and 5‐fold less sensitive to the activity of CheZ than was the wild‐type protein. Nonetheless, the phosphorylation and autodephos‐phorylation rates of these mutants, CheY23ND and CheY26KE, were observed to be identical to those of wild‐type CheY in the absence of CheZ. These are the first examples of CheY mutations that reduce sensitivity to the phosphatase activity of CheZ without being altered in terms of their intrinsic phosphorylation and autodephospborylation rates, interestingly, the residues Asn‐23 and Lys‐26 are located on a face of CheY far from the phosphorylation site (Asp‐57), distinct from the previously described site of inter‐action with the histidine kinase CheA, and partially overlapping with a region implicated in interaction with the flagellar switch.

Bibliography

Sanna, M. G., Swanson, R. V., Bourret, R. B., & Simon, M. I. (1995). Mutations in the chemotactic response regulator, CheY, that confer resistance to the phosphatase activity of CheZ. Molecular Microbiology, 15(6), 1069â1079. Portico.

Authors 4

M. Germana Sanna (first)
Ronald V. Swanson (additional)
Robert B. Bourret (additional)
Melvin I. Simon (additional)

References 46 Referenced 35

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{'key': 'e_1_2_1_20_1', 'first-page': '11', 'article-title': 'A method for random mutagenesis of a defined DNA segment using a modified polymerase chain reaction', 'volume': '1', 'author': 'Leung D.W.', 'year': '1989', 'journal-title': 'Technique'} / Technique / A method for random mutagenesis of a defined DNA segment using a modified polymerase chain reaction by Leung D.W. (1989)
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10.1016/S0021-9258(18)92982-0
10.1073/pnas.89.2.718 / Proc Natl Acad Sci USA / Phosphorylalion of bacterial response regulator proteins by low molecular weight phospho‐donors by Lukat G.S. (1992)
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10.1128/JB.135.1.45-53.1978
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10.1128/jb.174.19.6247-6255.1992
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10.1016/0378-1119(85)90120-9

Dates

Type	When
Created	18 years, 9 months ago (Oct. 27, 2006, 11:06 p.m.)
Deposited	1 year, 9 months ago (Oct. 26, 2023, 4:02 p.m.)
Indexed	1 year, 4 months ago (March 26, 2024, 7:06 a.m.)
Issued	30 years, 5 months ago (March 1, 1995)
Published	30 years, 5 months ago (March 1, 1995)
Published Online	18 years, 9 months ago (Oct. 27, 2006)
Published Print	30 years, 5 months ago (March 1, 1995)

Funders 0

None

BibTeX

@article{Sanna_1995, title={Mutations in the chemotactic response regulator, CheY, that confer resistance to the phosphatase activity of CheZ}, volume={15}, ISSN={1365-2958}, url={http://dx.doi.org/10.1111/j.1365-2958.1995.tb02282.x}, DOI={10.1111/j.1365-2958.1995.tb02282.x}, number={6}, journal={Molecular Microbiology}, publisher={Wiley}, author={Sanna, M. Germana and Swanson, Ronald V. and Bourret, Robert B. and Simon, Melvin I.}, year={1995}, month=mar, pages={1069–1079} }

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