DOI: 10.1111/j.1365-2958.1995.tb02258.x. Induction of phospholipase‐ and flagellar synthesis in <i>Serratia liquefaciens</i> is controlled by expression of the flagellar master operon <i>flhD</i>

Induction of phospholipase‐ and flagellar synthesis in Serratia liquefaciens is controlled by expression of the flagellar master operon flhD

10.1111/j.1365-2958.1995.tb02258.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

SummaryWhen a liquid culture of Serratia spp. reaches the last part of the logarithmic phase of growth it induces the synthesis of several extracellular hydrolytic enzymes. In this communication we show that synthesis and secretion of the extracellular phospholipase is coupled to expression of flagella. Expression of flagella is demonstrated to follow a growth‐phase‐dependent pattern. Cloning, complementation studies and DNA‐sequencing analysis has identified a genetic region in Serratia liquefaciens which exhibits extensive homology to the Escherichia coli flhD flagellar master operon. Interruption of the chromosomal flhD operon in S. liquefaciens results in non‐flagellated and phospholipase‐negative cells, but the synthesis of other exoenzymes is not affected. By placing the flhD operon under the control of a foreign inducible promoter we have shown that increased transcription through the flhD operon leads to induction of flagellar synthesis and phospholipase expression.

Bibliography

Givskov, M., Eberl, L., Christiansen, G., Benedik, M. J., & Molin, S. (1995). Induction of phospholipaseâ and flagellar synthesis in Serratia liquefaciens is controlled by expression of the flagellar master operon flhD. Molecular Microbiology, 15(3), 445â454. Portico.

Authors 5

Michael Givskov (first)
Leo Eberl (additional)
Gunna Christiansen (additional)
Michael J. Benedik (additional)
Søren Molin (additional)

References 25 Referenced 83

10.1128/jb.175.19.6238-6244.1993
10.1128/jb.172.1.342-349.1990 / J Bacteriol / Expression of Serratia marcescens extracellular proteins requires recA by Ball T.K. (1990)
10.1128/jb.170.4.1575-1581.1988
10.1128/jb.62.3.293-300.1951 / J Bacteriol / Studies of lysogenesis, I. The mode of phage liberation by lysogenic Eschericia coli by Bertani G. (1951)
10.1111/j.1365-2958.1992.tb00857.x
10.1111/j.1365-2958.1993.tb01567.x
10.1128/jb.170.12.5855-5862.1988 / J Bacteriol / Cloning and expression in Eschericia coli of the gene for an extracellular phospholipase from Serratia liquefaciens by Givskov M. (1988)
10.1111/j.1365-2958.1991.tb01847.x
10.1128/jb.140.3.1120-1122.1979 / J Bacteriol / Lipolytic activity copurified with the outer membrane of Serratia marcescens by Heller K.B. (1979)
10.1128/jb.172.11.6557-6567.1990
10.1007/BF00328136
10.1128/jb.150.1.16-26.1982 / J Bacteriol / Fusion of flagellar operons to lactose genes on a Mu lac bacteriophage by Komeda Y. (1982)
10.1128/jb.173.14.4474-4481.1991
10.1128/jb.173.7.2160-2166.1991
10.1128/jb.172.11.6568-6572.1990
10.1016/0378-1119(93)90533-9
10.1146/annurev.ge.26.120192.001023
{'key': 'e_1_2_1_19_1', 'volume-title': 'Experiments in Molecular Genetics. Cold', 'author': 'Miller J.H.', 'year': '1972'} / Experiments in Molecular Genetics. Cold by Miller J.H. (1972)
10.1128/jb.170.6.2575-2583.1988
10.1093/nar/11.22.8019
10.1128/jb.174.19.6256-6263.1992
10.1146/annurev.mi.31.100177.002145
10.1046/j.1365-2958.2003.03670.x
10.1016/0378-1119(89)90262-X
10.1128/jb.138.3.663-670.1979 / J Bacteriol / Glycogen, hyaluronate, and some polysaccharides greatly enhance the formation of exolipase by Serratia marcescens by Winkler U. (1979)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 27, 2006, 11 p.m.)
Deposited	1 year, 10 months ago (Oct. 25, 2023, 6:45 p.m.)
Indexed	1 year, 1 month ago (Aug. 3, 2024, 9:23 a.m.)
Issued	30 years, 7 months ago (Feb. 1, 1995)
Published	30 years, 7 months ago (Feb. 1, 1995)
Published Online	18 years, 10 months ago (Oct. 27, 2006)
Published Print	30 years, 7 months ago (Feb. 1, 1995)

Funders 0

None

BibTeX

@article{Givskov_1995, title={Induction of phospholipase‐ and flagellar synthesis in Serratia liquefaciens is controlled by expression of the flagellar master operon flhD}, volume={15}, ISSN={1365-2958}, url={http://dx.doi.org/10.1111/j.1365-2958.1995.tb02258.x}, DOI={10.1111/j.1365-2958.1995.tb02258.x}, number={3}, journal={Molecular Microbiology}, publisher={Wiley}, author={Givskov, Michael and Eberl, Leo and Christiansen, Gunna and Benedik, Michael J. and Molin, Søren}, year={1995}, month=feb, pages={445–454} }

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