DOI: 10.1111/j.1365-2958.1994.tb01280.x. Iron, DtxR, and the regulation of diphtheria toxin expression

Iron, DtxR, and the regulation of diphtheria toxin expression

10.1111/j.1365-2958.1994.tb01280.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

SummaryIn recent years considerable advances have been made in the understanding of the molecular basis of iron‐mediated regulation of diphtheria toxin expression. The tox gene has been shown to be regulated by the heavy metal ion‐activated regulatory element DtxR. In the presence of divalent heavy metal ions, DtxR becomes activated and binds to a 9 bp interrupted palindromic sequence. The consensus‐binding site has been determined by both the sequence analysis of DtxR‐responsive operators cloned from genomic libraries of Corynebacterium diphtheriae as well as by in vitro genetic methods using cyclic amplification of selected targets (CAST‐ing). it is now clear that DtxR functions as a global iron‐sensitive regulatory element in the control of gene expression in C. diphtheriae. In addition, the metal ion‐activation domain of DtxR is being characterized by both mutational analysis and determination of the X‐ray structure at 3.0 Å resolution.

Bibliography

Tao, X., Schiering, N., Zeng, H., Ringe, D., & Murphy, J. R. (1994). Iron, DtxR, and the regulation of diphtheria toxin expression. Molecular Microbiology, 14(2), 191â197. Portico.

Authors 5

Xu Tao (first)
Nikolaus Schiering (additional)
Hui‐yan Zeng (additional)
Dagmar Ringe (additional)
John R. Murphy (additional)

References 44 Referenced 119

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 27, 2006, 10:44 p.m.)
Deposited	2 years ago (Sept. 5, 2023, 2:52 a.m.)
Indexed	2 months, 1 week ago (June 26, 2025, 9:10 a.m.)
Issued	30 years, 11 months ago (Oct. 1, 1994)
Published	30 years, 11 months ago (Oct. 1, 1994)
Published Online	18 years, 10 months ago (Oct. 27, 2006)
Published Print	30 years, 11 months ago (Oct. 1, 1994)

Funders 0

None

BibTeX

@article{Tao_1994, title={Iron, DtxR, and the regulation of diphtheria toxin expression}, volume={14}, ISSN={1365-2958}, url={http://dx.doi.org/10.1111/j.1365-2958.1994.tb01280.x}, DOI={10.1111/j.1365-2958.1994.tb01280.x}, number={2}, journal={Molecular Microbiology}, publisher={Wiley}, author={Tao, Xu and Schiering, Nikolaus and Zeng, Hui‐yan and Ringe, Dagmar and Murphy, John R.}, year={1994}, month=oct, pages={191–197} }

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