DOI: 10.1111/j.1365-2958.1994.tb01085.x. Regulation of acetyl phosphate synthesis and degradation, and the control of flagellar expression in <i>Escherichia coli</i>

Regulation of acetyl phosphate synthesis and degradation, and the control of flagellar expression in Escherichia coli

10.1111/j.1365-2958.1994.tb01085.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

SummaryWe investigated the relationship between Escherichia coli flagellar expression and the regulation of acetyl phosphate synthesis and degradation. Using cells either wild type for acetyl phosphate metabolism or defective for phosphotransacetylase or acetate kinase, or both, we measured flagellar expression and the intracellular concentration of acetyl phosphate relative to growth phase and temperature. Under the conditions tested, we found that elevated levels of acetyl phosphate corresponded to inhibition of flagellar synthesis. To extend these observations, we measured the intracellular concentration of acetyl‐CoA, the level of expression from the pta and ackA promoters, and the activities of phosphotransacetylase and acetate kinase derived from cell lysates. Relative to increasing culture density, acetyl‐CoA levels and expression from both the pta and ackA promoters decreased. Relative to Increasing temperature, expression from the ackA promoter decreased and phosphotransacetylase activity increased. In contrast, temperature had little or no effect on either acetate kinase activity or expression from the pta promoter. We propose that cells regulate intracellular acetyl phosphate concentrations relative to growth phase and temperature by modulating the availability of acetyl‐CoA, the expression of ackA, and the activity of phosphotransacetylase.

Bibliography

PrÃ¼Î², B. M., & Wolfe, A. J. (1994). Regulation of acetyl phosphate synthesis and degradation, and the control of flagellar expression in Escherichia coli. Molecular Microbiology, 12(6), 973â984. Portico.

Authors 2

Birgtt M. Prüβ (first)
Alan J. Wolfe (additional)

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 27, 2006, 10:24 p.m.)
Deposited	1 year, 10 months ago (Oct. 24, 2023, 7:50 p.m.)
Indexed	1 month, 3 weeks ago (July 12, 2025, 6:45 p.m.)
Issued	31 years, 3 months ago (June 1, 1994)
Published	31 years, 3 months ago (June 1, 1994)
Published Online	18 years, 10 months ago (Oct. 27, 2006)
Published Print	31 years, 3 months ago (June 1, 1994)

Funders 0

None

BibTeX

@article{Pr__1994, title={Regulation of acetyl phosphate synthesis and degradation, and the control of flagellar expression in Escherichia coli}, volume={12}, ISSN={1365-2958}, url={http://dx.doi.org/10.1111/j.1365-2958.1994.tb01085.x}, DOI={10.1111/j.1365-2958.1994.tb01085.x}, number={6}, journal={Molecular Microbiology}, publisher={Wiley}, author={Prüβ, Birgtt M. and Wolfe, Alan J.}, year={1994}, month=jun, pages={973–984} }

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