DOI: 10.1111/j.1365-2958.1993.tb01695.x. A pleiotropic reduced virulence (Rvi−) mutant of Erwinia carotovora subspecies atroseptica is defective in flagella assembly proteins that are conserved in plant and animal bacterial pathogens

A pleiotropic reduced virulence (Rvi−) mutant of Erwinia carotovora subspecies atroseptica is defective in flagella assembly proteins that are conserved in plant and animal bacterial pathogens

10.1111/j.1365-2958.1993.tb01695.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

Summary Erwinia carotovora subsp. atroseptica was mutage‐nized and assayed for virulence in planta. Those mutants which exhibited reduced virulence (Rvi‐) were assayed for growth rate, auxotrophy and extracellular enzyme secretion and seven mutants were found to be wild type for all of these phenotypes. When screened for other phenotypes, two were found to be non‐motile. One mutant was complemented for motility by a heterologous gene library. A 2.7kb XmaIII‐Clal complementing fragment was sequenced and the gene products were found to have similarity to flagella biosynthesis gene products from several bacteria. Further similarity was found to a pathogenicity protein from the plant pathogen Xanthomonas campestris pv. glycines and to the Spa pathogenicity proteins of the human pathogen Shigella fiexneri, which are involved in the surface presentation of antigens. These studies highlight the emergence of common themes in the molecular strategies employed by both plant and animal bacterial pathogens for the targeting of proteins involved in the elaboration of disease.

Bibliography

Mulholland, V., Hinton, J. C. D., Sidebotham, J., Toth, I. K., Hyman, L. J., Perombelon, M. C. M., Reeves, P. J., & Salmond, G. P. C. (1993). A pleiotropic reduced virulence (Rviâ) mutant of Erwinia carotovora subspecies atroseptica is defective in flagella assembly proteins that are conserved in plant and animal bacterial pathogens. Molecular Microbiology, 9(2), 343â356. Portico.

Authors 8

Vincent Mulholland (first)
Jay C. D. Hinton (additional)
Julie Sidebotham (additional)
Ian K. Toth (additional)
Lisabeth J. Hyman (additional)
Michel C. M. Perombelon (additional)
Philip J. Reeves (additional)
George P. C. Salmond (additional)

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 27, 2006, 9:42 p.m.)
Deposited	1 year, 10 months ago (Oct. 25, 2023, 11:24 a.m.)
Indexed	1 year, 3 months ago (May 30, 2024, 8:50 p.m.)
Issued	32 years, 2 months ago (July 1, 1993)
Published	32 years, 2 months ago (July 1, 1993)
Published Online	18 years, 10 months ago (Oct. 27, 2006)
Published Print	32 years, 2 months ago (July 1, 1993)

Funders 0

None

BibTeX

@article{Mulholland_1993, title={A pleiotropic reduced virulence (Rvi−) mutant of Erwinia carotovora subspecies atroseptica is defective in flagella assembly proteins that are conserved in plant and animal bacterial pathogens}, volume={9}, ISSN={1365-2958}, url={http://dx.doi.org/10.1111/j.1365-2958.1993.tb01695.x}, DOI={10.1111/j.1365-2958.1993.tb01695.x}, number={2}, journal={Molecular Microbiology}, publisher={Wiley}, author={Mulholland, Vincent and Hinton, Jay C. D. and Sidebotham, Julie and Toth, Ian K. and Hyman, Lisabeth J. and Perombelon, Michel C. M. and Reeves, Philip J. and Salmond, George P. C.}, year={1993}, month=jul, pages={343–356} }

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