DOI: 10.1111/j.1365-2958.1993.tb01190.x. Ribosome activity and modification of 16S RNA are influenced by deletion of ribosomal protein S20

Ribosome activity and modification of 16S RNA are influenced by deletion of ribosomal protein S20

10.1111/j.1365-2958.1993.tb01190.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

SummaryA spontaneous mutant of Escherichia coli K‐12 was isolated that shows an increased misreading ability of all three nonsense codons together with an inability to grow at 42° C. It is demonstrated that the mutation is a deletion of the gene rpsT, coding for ribosomal protein S20. The loss of this protein not only influences the decoding properties of the ribosome; the modification pattern of 16S ribosomal RNA is also changed. This leads to a deficiency in the ability of the mutant to associate its 30S subunits with 50S subunits to form 70S ribosomes. It is suggested that two modified bases, m5C and m62A, are directly or indirectly essential for association of subunits to functional ribosomes in the rpsT mutant strain. Two other modifications were also studied; m2G which is not affected at all and m3U which is undermodified in both active and inactive subunits and, therefore, not involved in subunit association.

Bibliography

RydÃ©nâAulin, M., Shaoping, Z., Kylsten, P., & Isaksson, L. A. (1993). Ribosome activity and modification of 16S RNA are influenced by deletion of ribosomal protein S20. Molecular Microbiology, 7(6), 983â992. Portico.

Authors 4

Monica Rydén‐Aulin (first)
Zhang Shaoping (additional)
Per Kylsten (additional)
Leif A. Isaksson (additional)

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 27, 2006, 9:14 p.m.)
Deposited	1 year, 11 months ago (Sept. 28, 2023, 1:54 a.m.)
Indexed	1 year, 1 month ago (Aug. 3, 2024, 5:16 a.m.)
Issued	32 years, 6 months ago (March 1, 1993)
Published	32 years, 6 months ago (March 1, 1993)
Published Online	18 years, 10 months ago (Oct. 27, 2006)
Published Print	32 years, 6 months ago (March 1, 1993)

Funders 0

None

BibTeX

@article{Ryd_n_Aulin_1993, title={Ribosome activity and modification of 16S RNA are influenced by deletion of ribosomal protein S20}, volume={7}, ISSN={1365-2958}, url={http://dx.doi.org/10.1111/j.1365-2958.1993.tb01190.x}, DOI={10.1111/j.1365-2958.1993.tb01190.x}, number={6}, journal={Molecular Microbiology}, publisher={Wiley}, author={Rydén‐Aulin, Monica and Shaoping, Zhang and Kylsten, Per and Isaksson, Leif A.}, year={1993}, month=mar, pages={983–992} }

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        [
          1993,
          3
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1111/j.1365-2958.1993.tb01190.x"
  ],
  "URL": "http://dx.doi.org/10.1111/j.1365-2958.1993.tb01190.x",
  "relation": {},
  "ISSN": [
    "0950-382X",
    "1365-2958"
  ],
  "subject": [],
  "container-title-short": "Molecular Microbiology",
  "published": {
    "date-parts": [
      [
        1993,
        3
      ]
    ]
  }
}