DOI: 10.1111/j.1365-2958.1993.tb01111.x. Use of an ordered cosmid library to deduce the genomic organization of <i>Mycobacterium leprae</i>

Use of an ordered cosmid library to deduce the genomic organization of Mycobacterium leprae

10.1111/j.1365-2958.1993.tb01111.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

SummaryIn an attempt to unify the genetic and biological research on Mycobacterium leprae, the aetiological agent of leprosy, a cosmid library was constructed and then ordered by a combination of fingerprinting and hybridization techniques. The genome of M. leprae is represented by four contigs of overlapping clones which, together, account for nearly 2.B Mb of DNA. Several arguments suggest that the gaps between the contigs are small in size and that virtually complete coverage of the chromosome has been obtained. All of the cloned M. leprae genes have been positioned on the contig maps together with the 29 copies of the dispersed repetitive element, RLEP. These have been classified into four groups on the basis of differences in their organization. Several key housekeeping genes were identified and mapped by hybridization with heterologous probes, and the current genome map of this uncultivable pathogen comprises 72 loci.

Bibliography

Eiglmeier, K., HonorÃ©, N., Woods, S. A., Caudron, B., & Cole, S. T. (1993). Use of an ordered cosmid library to deduce the genomic organization of Mycobacterium leprae. Molecular Microbiology, 7(2), 197â206. Portico.

Authors 5

K. Eiglmeier (first)
N. Honoré (additional)
S. A. Woods (additional)
B. Caudron (additional)
S. T. Cole (additional)

References 52 Referenced 95

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 27, 2006, 9:05 p.m.)
Deposited	1 year, 10 months ago (Oct. 25, 2023, 12:58 a.m.)
Indexed	3 months, 2 weeks ago (May 18, 2025, 10:25 a.m.)
Issued	32 years, 8 months ago (Jan. 1, 1993)
Published	32 years, 8 months ago (Jan. 1, 1993)
Published Online	18 years, 10 months ago (Oct. 27, 2006)
Published Print	32 years, 8 months ago (Jan. 1, 1993)

Funders 0

None

BibTeX

@article{Eiglmeier_1993, title={Use of an ordered cosmid library to deduce the genomic organization of Mycobacterium leprae}, volume={7}, ISSN={1365-2958}, url={http://dx.doi.org/10.1111/j.1365-2958.1993.tb01111.x}, DOI={10.1111/j.1365-2958.1993.tb01111.x}, number={2}, journal={Molecular Microbiology}, publisher={Wiley}, author={Eiglmeier, K. and Honoré, N. and Woods, S. A. and Caudron, B. and Cole, S. T.}, year={1993}, month=jan, pages={197–206} }

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