DOI: 10.1111/j.1365-2958.1993.tb00924.x. The <i>rpsL</i> gene and streptomycin resistance in single and multiple drug‐resistant strains of <i>Mycobacterium tuberculosis</i>

The rpsL gene and streptomycin resistance in single and multiple drug‐resistant strains of Mycobacterium tuberculosis

10.1111/j.1365-2958.1993.tb00924.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

SummaryThe recent emergence of indolent and rapidly fatal drug‐resistant strains of Mycobacterium tuberculosis has renewed interest in defining the molecular mechanisms of drug resistance in the tubercle bacilli. In this report, we have examined the mechanism of resistance to streptomycin (Sm) in M. tuberculosis through the cloning and nucleotide sequence analysis of the gene encoding the ribosomal SR protein (rpsL gene) from streptomycin‐resistant strains and their streptomycin‐sensitive parental strains. We have demonstrated that five singly SmRM. tuberculosis strains and an SmR isolate that has reduced sensitivity to multiple antibiotics have identical point mutations at codon 43 of the rpsL gene. Mutations at this same site confer SmR in Escherichia coli. In contrast, two other multiple drug‐resistant M. tuberculosis strains that are resistant to Sm have rpsL genes that have the same nucleotide sequence as their drug‐sensitive parent strains, suggesting that different resistance mechanisms are involved in these strains.

Bibliography

Nair, J., Rouse, D. A., Bai, G., & Morris, S. L. (1993). The rpsL gene and streptomycin resistance in single and multiple drugâresistant strains of Mycobacterium tuberculosis. Molecular Microbiology, 10(3), 521â527. Portico.

Authors 4

Jaygopal Nair (first)
David A. Rouse (additional)
Gill‐Han Bai (additional)
Sheldon L. Morris (additional)

References 31 Referenced 134

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 27, 2006, 8:49 p.m.)
Deposited	1 year, 10 months ago (Oct. 25, 2023, 2:11 a.m.)
Indexed	2 months ago (July 3, 2025, 11:10 a.m.)
Issued	31 years, 10 months ago (Nov. 1, 1993)
Published	31 years, 10 months ago (Nov. 1, 1993)
Published Online	18 years, 10 months ago (Oct. 27, 2006)
Published Print	31 years, 10 months ago (Nov. 1, 1993)

Funders 0

None

BibTeX

@article{Nair_1993, title={The rpsL gene and streptomycin resistance in single and multiple drug‐resistant strains of Mycobacterium tuberculosis}, volume={10}, ISSN={1365-2958}, url={http://dx.doi.org/10.1111/j.1365-2958.1993.tb00924.x}, DOI={10.1111/j.1365-2958.1993.tb00924.x}, number={3}, journal={Molecular Microbiology}, publisher={Wiley}, author={Nair, Jaygopal and Rouse, David A. and Bai, Gill‐Han and Morris, Sheldon L.}, year={1993}, month=nov, pages={521–527} }

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