DOI: 10.1111/j.1365-2958.1992.tb01429.x. RecA protein of <i>Escherichia coli</i> and chromosome partitioning

RecA protein of Escherichia coli and chromosome partitioning

10.1111/j.1365-2958.1992.tb01429.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

Summary Escherichia coli cells deficient in RecA protein frequently contain an abnormal number of chromosomes after completion of ongoing rounds of DNA replication. This suggests that RecA protein may be required for correct timing of initiation of DNA replication; however, we show here that initiation of DNA replication is properly timed in recA mutants. We also find that more than 10% of recA mutant cells contain no DNA. These anucleate cells appear to arise from partitioning of all the DNA into one daughter cell and no DNA into the other daughter cell. Based on these and previously published results, we propose that RecA protein is required for equal partitioning of chromosomes into the two daughter cells.

Bibliography

Zyskind, J. W., Svitil, A. L., Stine, W. B., Biery, M. C., & Smith, D. W. (1992). RecA protein of Escherichia coli and chromosome partitioning. Molecular Microbiology, 6(17), 2525â2537. Portico.

Authors 5

Judith W. Zyskind (first)
Amy L. Svitil (additional)
W. Blaine Stine (additional)
Matthew C. Biery (additional)
Douglas W. Smith (additional)

References 74 Referenced 39

10.1128/jb.173.24.7970-7974.1991
10.1073/pnas.83.15.5558 / Proc Natl Acad Sei USA / recD: the gene for an essential third subunit of exonuclease V by Amundsen S. (1986)
10.1146/annurev.ge.10.120176.000413
10.1128/jb.171.10.5738-5742.1989
{'key': 'e_1_2_1_6_1', 'first-page': '475', 'article-title': 'Experiments on chromosome separation and positioning in Escherichia coli', 'volume': '3', 'author': 'Begg K.J.', 'year': '1991', 'journal-title': 'New Biologist'} / New Biologist / Experiments on chromosome separation and positioning in Escherichia coli by Begg K.J. (1991)
10.1128/jb.167.2.594-603.1986
10.1016/0092-8674(92)90446-J
{'key': 'e_1_2_1_9_1', 'first-page': '789', 'article-title': 'Escherichia coli XerC recombinase is required for chromosomal segregation at cell division', 'volume': '3', 'author': 'Blakely G.', 'year': '1991', 'journal-title': 'New Biologist'} / New Biologist / Escherichia coli XerC recombinase is required for chromosomal segregation at cell division by Blakely G. (1991)
10.1016/0092-8674(90)90272-G
10.1016/0923-2508(91)90020-B
10.1016/0022-2836(75)90371-X
10.1016/0300-9084(91)90124-J
{'key': 'e_1_2_1_14_1', 'first-page': '780', 'article-title': 'Site‐specific recombination promoted by a short DNA segment of plasmid R1 and by a homologous segment in the terminus region of the Escherichia coli chromosome', 'volume': '3', 'author': 'Clerget M.', 'year': '1991', 'journal-title': 'New Biologist'} / New Biologist / Site‐specific recombination promoted by a short DNA segment of plasmid R1 and by a homologous segment in the terminus region of the Escherichia coli chromosome by Clerget M. (1991)
10.1016/0022-2836(84)90141-4
10.1128/jb.171.10.5405-5409.1989
10.1111/j.1365-2958.1993.tb01182.x
10.1111/j.1365-2958.1989.tb00250.x
10.1101/SQB.1981.045.01.022 / Cold Spring Harb Symp Quant Biol / Identification of a sex‐factor‐affinity site in E. coli as γδ by Guyer M.S. (1981)
10.1016/0022-2836(87)90118-5
10.1016/0092-8674(88)90033-5
10.1016/S0021-9258(19)30040-7
10.1128/JB.173.1.391-393.1991
10.1016/0092-8674(88)90032-3
10.1073/pnas.86.5.1593
10.1128/jb.171.3.1496-1505.1989
10.1128/jb.172.1.31-39.1990
10.1111/j.1365-2958.1987.tb01932.x
10.1016/S0022-2836(05)80344-4
10.1007/BF00330959
10.1016/0092-8674(90)90172-B
10.1016/0092-8674(89)90012-3
10.1128/jb.163.2.439-444.1985
10.1007/BF00329857
10.1016/0092-8674(86)90490-3
10.1016/0300-9084(91)90216-N
{'key': 'e_1_2_1_37_1', 'first-page': '799', 'article-title': 'dif, a recA‐independent recombination site in the terminus region of the chromosome of Escherichia coli', 'volume': '3', 'author': 'Kuempel P.L.', 'year': '1991', 'journal-title': 'New Biologist'} / New Biologist / dif, a recA‐independent recombination site in the terminus region of the chromosome of Escherichia coli by Kuempel P.L. (1991)
10.1080/07391102.1989.10508505
10.1073/pnas.86.23.9104
10.1073/pnas.83.14.5101
10.1128/jb.170.5.2163-2173.1988
10.1016/0300-9084(91)90108-D
10.1073/pnas.77.6.3225
10.1016/0092-8674(89)90802-7
10.1128/jb.173.16.5097-5104.1991
10.1128/jb.172.4.1834-1839.1990
{'key': 'e_1_2_1_47_1', 'first-page': '299', 'article-title': 'recA gene as requirement for replication of Escherichia coli chromosome initiated by integrated F′ plasmid', 'volume': '15', 'author': 'Mao Y.‐M.', 'year': '1988', 'journal-title': 'Chin J Genet'} / Chin J Genet / recA gene as requirement for replication of Escherichia coli chromosome initiated by integrated F′ plasmid by Mao Y.‐M. (1988)
10.1007/BF00269854
10.1016/0022-2836(84)90142-6
10.1073/pnas.74.7.2720
{'volume-title': 'Experiments in Molecular Genetics', 'year': '1972', 'author': 'Miller J.H.', 'key': 'e_1_2_1_51_1'} / Experiments in Molecular Genetics by Miller J.H. (1972)
10.1128/jb.109.3.971-978.1972 / J Bacteriol / Conditional lethality of recA and recB derivatives of a strain of Escherichia coli K‐12 with a temperature‐sensitive deoxyribonucleic acid polymerase I by Monk M. (1972)
10.1128/jb.112.2.886-893.1972 / J Baeteriol / Dominant mutants (lexA) in Escherichia coli K‐12 which affect radiation sensitivity and frequency of ultraviolet light‐induced mutations by Mount D.W. (1972)
10.1101/SQB.1968.033.01.062
10.1128/jb.119.3.736-747.1974
10.1002/j.1460-2075.1991.tb07935.x
10.1128/jb.173.21.6686-6693.1991
10.1038/290422a0
{'key': 'e_1_2_1_59_1', 'first-page': '415', 'article-title': 'The RecA protein: structure and function', 'volume': '25', 'author': 'Rooa A.I.', 'year': '1991', 'journal-title': 'Crit Rev Biochem Mol Biol'} / Crit Rev Biochem Mol Biol / The RecA protein: structure and function by Rooa A.I. (1991)
10.1016/0022-2836(90)90306-7 / J Mol Biol / Nature of the SOS‐inducing signal in Escherichia coli by Sassanfar M. (1990)
10.1016/0092-8674(92)90447-K
10.1073/pnas.86.9.3026
10.1128/jb.170.6.2549-2554.1988 / J Bacteriol / Perturbed chromosomal replication in recA mutants of Escherichia coli by Skarstad K. (1988)
10.1002/j.1460-2075.1986.tb04415.x / EMB0 J / Timing of initiation of chromosome replication in individual Escherichia coli cells by Skarstad K. (1986)
10.1128/jb.170.2.852-858.1988
10.1128/jb.174.9.3078-3082.1992 / J Baeteriol / Escherichia coli cells lacking methylation‐blocking factor (MBF)/leucine‐responsive regulatory protein (LRP) have precise timing of initiation of DNA replication in the cell cycle by Smith D.W. (1992)
10.1016/0092-8674(84)90058-8
10.1016/0300-9084(91)90111-D
10.1007/BF00328133
10.1128/jb.148.1.386-390.1981 / J Bacteriol / Overproduction of the Escherichia coli recA protein without stimulation of its proteolytic activity by Uhlin B.E. (1981)
10.1073/pnas.87.12.4776
10.1128/jb.161.3.1219-1221.1985
10.1016/0300-9084(91)90196-8
10.1073/pnas.81.23.7539
10.1128/jb.131.1.123-132.1977 / J Bacteriol / Analysis of genetic recombination between two partially deleted lactose operons of Escherichia coli K‐12 by Zieg J. (1977)

Dates

Type	When
Created	15 years ago (Aug. 18, 2010, 7:40 a.m.)
Deposited	1 year, 9 months ago (Oct. 23, 2023, 2:19 p.m.)
Indexed	1 month ago (July 16, 2025, 8:42 a.m.)
Issued	32 years, 11 months ago (Sept. 1, 1992)
Published	32 years, 11 months ago (Sept. 1, 1992)
Published Online	18 years, 9 months ago (Oct. 27, 2006)
Published Print	32 years, 11 months ago (Sept. 1, 1992)

Funders 0

None

BibTeX

@article{Zyskind_1992, title={RecA protein of Escherichia coli and chromosome partitioning}, volume={6}, ISSN={1365-2958}, url={http://dx.doi.org/10.1111/j.1365-2958.1992.tb01429.x}, DOI={10.1111/j.1365-2958.1992.tb01429.x}, number={17}, journal={Molecular Microbiology}, publisher={Wiley}, author={Zyskind, Judith W. and Svitil, Amy L. and Stine, W. Blaine and Biery, Matthew C. and Smith, Douglas W.}, year={1992}, month=sep, pages={2525–2537} }

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