DOI: 10.1111/j.1365-2958.1992.tb01392.x. Osmostress‐induced changes in yeast gene expression

Osmostress‐induced changes in yeast gene expression

10.1111/j.1365-2958.1992.tb01392.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

SummaryWhen Saccharomyces cerevisiae cells are exposed to high concentrations of NaCI, they show reduced viability, methionine uptake and protein biosynthesis. Cells can acquire tolerance against a severe salt shock (up to 1.4 M NaCI) by a previous treatment with 0.7 M NaCI, but not by a previous heat shock. Two‐dimensional analysis of [3H]‐leucine‐labelled proteins from salt‐shocked cells (0.7 M NaCt) revealed the elevated rate of synthesis of nine proteins, among which were the heat‐shock proteins hsp12 and hsp26. Northern analysis using gene‐specific probes confirmed the identity of the latter proteins and, in addition, demonstrated the induction of glycerol‐3‐phos‐phate dehydrogenase gene expression. The synthesis of the same set of proteins is induced or enhanced upon exposure of cells to 0.8 M sucrose, although not as dramatically as in an iso‐osmolar NaCI concentration (0.7 M).

Bibliography

Varela, J. C. S., van Beekvelt, C., Planta, R. J., & Mager, W. H. (1992). Osmostressâinduced changes in yeast gene expression. Molecular Microbiology, 6(15), 2183â2190. Portico.

Authors 4

Joäo C. S. Varela (first)
Catelijne van Beekvelt (additional)
Rudi J. Planta (additional)
Willem H. Mager (additional)

References 46 Referenced 84

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{'key': 'e_1_2_1_19_1', 'first-page': '339', 'article-title': 'Osmotic regulation of gene expression: ionic strength as an intracellular signal', 'volume': '12', 'author': 'Higgins C.F.', 'year': '1987', 'journal-title': 'Trends Genet'} / Trends Genet / Osmotic regulation of gene expression: ionic strength as an intracellular signal by Higgins C.F. (1987)
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{'key': 'e_1_2_1_29_1', 'first-page': '3049', 'article-title': 'Osmotic adjustment and the accumulation of organic solutes in whole cells and protoplasts of Saccharomyces cerevisiae', 'volume': '134', 'author': 'Meikle A.J.', 'year': '1988', 'journal-title': 'J Gen Microbiol'} / J Gen Microbiol / Osmotic adjustment and the accumulation of organic solutes in whole cells and protoplasts of Saccharomyces cerevisiae by Meikle A.J. (1988)
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Dates

Type	When
Created	18 years, 10 months ago (Oct. 27, 2006, 7:05 p.m.)
Deposited	1 year, 10 months ago (Oct. 23, 2023, 4:49 p.m.)
Indexed	3 weeks, 5 days ago (Aug. 5, 2025, 8:41 a.m.)
Issued	33 years ago (Aug. 1, 1992)
Published	33 years ago (Aug. 1, 1992)
Published Online	18 years, 10 months ago (Oct. 27, 2006)
Published Print	33 years ago (Aug. 1, 1992)

Funders 0

None

BibTeX

@article{Varela_1992, title={Osmostress‐induced changes in yeast gene expression}, volume={6}, ISSN={1365-2958}, url={http://dx.doi.org/10.1111/j.1365-2958.1992.tb01392.x}, DOI={10.1111/j.1365-2958.1992.tb01392.x}, number={15}, journal={Molecular Microbiology}, publisher={Wiley}, author={Varela, Joäo C. S. and van Beekvelt, Catelijne and Planta, Rudi J. and Mager, Willem H.}, year={1992}, month=aug, pages={2183–2190} }

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