DOI: 10.1111/j.1365-2958.1991.tb02109.x. The stringent response in <i>Streptomyces coelicolor</i> A3(2)

The stringent response in Streptomyces coelicolor A3(2)

10.1111/j.1365-2958.1991.tb02109.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

SummaryThe stringent response was elicited in the antibiotic producer Streptomyces coelicolor A3(2) either by amino acid depletion (nutritional shiftdown) or by the addition of serine hydroxamate; both led to increased levels of ppGpp and to a reduction in transcription from the four promoters of the rrnD rRNA gene set. Analysis of untreated batch cultures revealed elevated ppGpp levels at the end of exponential growth, preceding the onset of antibiotic production. The effect of provoking the stringent response on antibiotic production in exponentially growing cultures was assessed by S1 nuclease mapping of actIII, an early gene of the actinorhodin biosynthetic cluster. Expression of act III occurred after nutritional shiftdown, but not after treatment with serine hydroxamate. Although the need for ppGpp in triggering antibiotic production remains equivocal, ppGpp synthesis atone does not appear to be sufficient to initiate secondary metabolism in S. coelicolor A3(2).

Bibliography

Strauch, E., Takano, E., Baylts, H. A., & Bibb, M. J. (1991). The stringent response in Streptomyces coelicolor A3(2). Molecular Microbiology, 5(2), 289â298. Portico.

Authors 4

E. Strauch (first)
E. Takano (additional)
H. A. Baylts (additional)
M. J. Bibb (additional)

References 37 Referenced 149

10.1128/jb.170.12.5642-5646.1988 / J Bacteriol / Induction of cat‐86 by chloramphenicol and amino acid starvation in relaxed mutants of Bacillus subtilis by Ambulos N.P. (1988)
10.1128/jb.138.2.530-534.1979 / J Bacteriol / Escherichia coli mutant containing a large deletion from relA to argA by Atherley A.G. (1979)
{'key': 'e_1_2_1_4_1', 'first-page': 'B508', 'volume-title': 'Shotgun DNA sequencing. In Techniques in Nucleic Acid Biochemistry.', 'author': 'Bankier A.T.', 'year': '1983'} / Shotgun DNA sequencing. In Techniques in Nucleic Acid Biochemistry. by Bankier A.T. (1983)
10.1007/BF00330593
10.1111/j.1365-2958.1988.tb00065.x
10.1016/0378-1119(86)90122-8
{'key': 'e_1_2_1_8_1', 'first-page': '1410', 'volume-title': 'Cellular and Molecular Biology.', 'author': 'Cashel M.', 'year': '1987'} / Cellular and Molecular Biology. by Cashel M. (1987)
10.1111/j.1365-2958.1990.tb00579.x
{'key': 'e_1_2_1_10_1', 'first-page': '257', 'volume-title': 'Microbiology: Including Immunology and Molecular Genetics.', 'author': 'Davis B.D.', 'year': '1980'} / Microbiology: Including Immunology and Molecular Genetics. by Davis B.D. (1980)
{'key': 'e_1_2_1_11_1', 'first-page': '127', 'volume-title': 'Regulation of Secondary Metabolism in Actinomycetes.', 'author': 'Demain A.L.', 'year': '1989'} / Regulation of Secondary Metabolism in Actinomycetes. by Demain A.L. (1989)
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{'key': 'e_1_2_1_16_1', 'volume-title': 'Genetic Manipulation of Streptomyces: A Laboratory Manual.', 'author': 'Hopwood D.A.', 'year': '1985'} / Genetic Manipulation of Streptomyces: A Laboratory Manual. by Hopwood D.A. (1985)
10.1016/S0076-6879(80)65047-2
{'key': 'e_1_2_1_18_1', 'first-page': '128', 'volume-title': 'Sporulation and Germination.', 'author': 'Lopez J.M.', 'year': '1981'} / Sporulation and Germination. by Lopez J.M. (1981)
10.1016/0003-2697(86)90605-6
{'key': 'e_1_2_1_20_1', 'first-page': '2621', 'article-title': 'Occurrence of the stringent response in Streptomyces sp. and its significance for the initiation of morphological and physiological differentiation', 'volume': '132', 'author': 'Ochi K.', 'year': '1986', 'journal-title': 'J Gen Microbiol'} / J Gen Microbiol / Occurrence of the stringent response in Streptomyces sp. and its significance for the initiation of morphological and physiological differentiation by Ochi K. (1986)
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10.1128/jb.169.8.3608-3616.1987
{'key': 'e_1_2_1_23_1', 'first-page': '2787', 'article-title': 'Changes in nucleotide pools during sporulation of Streptomyces griseus in submerged culture', 'volume': '133', 'author': 'Ochi K.', 'year': '1987', 'journal-title': 'J Gen Microbiol'} / J Gen Microbiol / Changes in nucleotide pools during sporulation of Streptomyces griseus in submerged culture by Ochi K. (1987)
{'key': 'e_1_2_1_24_1', 'first-page': '330', 'volume-title': 'Biology of Actinomyces', 'author': 'Ochi K.', 'year': '1988'} / Biology of Actinomyces by Ochi K. (1988)
10.1016/S0021-9258(17)37860-2 / J Biol Chem / A new nucleotide involved in the stringent response in Escherichia coli by Pao C.C. (1979)
{'key': 'e_1_2_1_26_1', 'first-page': '2549', 'article-title': 'Effect of serine hydroxamate and methyl α‐D‐glucopyranoside treatment on nucleoside polyphosphate pools, RNA and protein accumulation in Streptomyces hygroscopicus', 'volume': '130', 'author': 'Riesenberg D.', 'year': '1984', 'journal-title': 'J Gen Microbiol'} / J Gen Microbiol / Effect of serine hydroxamate and methyl α‐D‐glucopyranoside treatment on nucleoside polyphosphate pools, RNA and protein accumulation in Streptomyces hygroscopicus by Riesenberg D. (1984)
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10.1016/0092-8674(83)90314-8
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{'key': 'e_1_2_1_31_1', 'first-page': '760', 'article-title': 'Transcription map of adenovirus', 'volume': '65', 'author': 'Sharp P.A.', 'year': '1980', 'journal-title': 'Meth Enzymol'} / Meth Enzymol / Transcription map of adenovirus by Sharp P.A. (1980)
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10.1128/jb.106.3.972-982.1971 / J Bacteriol / Biochemical bases for the antimetabolite action of L‐serine hydroxamate by Tosa T. (1971)
10.7164/antibiotics.38.128
10.1128/jb.169.1.26-32.1987

Dates

Type	When
Created	18 years, 10 months ago (Oct. 27, 2006, 5:52 p.m.)
Deposited	1 year, 10 months ago (Oct. 23, 2023, 12:33 a.m.)
Indexed	3 months, 1 week ago (May 18, 2025, 10:24 a.m.)
Issued	34 years, 6 months ago (Feb. 1, 1991)
Published	34 years, 6 months ago (Feb. 1, 1991)
Published Online	18 years, 10 months ago (Oct. 27, 2006)
Published Print	34 years, 6 months ago (Feb. 1, 1991)

Funders 0

None

BibTeX

@article{Strauch_1991, title={The stringent response in Streptomyces coelicolor A3(2)}, volume={5}, ISSN={1365-2958}, url={http://dx.doi.org/10.1111/j.1365-2958.1991.tb02109.x}, DOI={10.1111/j.1365-2958.1991.tb02109.x}, number={2}, journal={Molecular Microbiology}, publisher={Wiley}, author={Strauch, E. and Takano, E. and Baylts, H. A. and Bibb, M. J.}, year={1991}, month=feb, pages={289–298} }

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