DOI: 10.1111/j.1365-2958.1990.tb02036.x. Rates of assembly and degradation of bacterial ice nuclei

Rates of assembly and degradation of bacterial ice nuclei

10.1111/j.1365-2958.1990.tb02036.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

SummaryThe kinetics of ice‐nucleus assembly from newly synthesized nucleation protein were observed following induction of nucleation gene expression in the heterologous host Escherichia coli. Assembly was significantly slower for the small proportion of ice nuclei active above ‐4.4°C; this was consistent with the belief that these nuclei comprise the largest aggregates of nucleation protein. The kinetics of nucleus degradation were followed after inhibiting protein synthesis. Nucleation activity and protein showed a concerted decay, indicating that most of the functional ice nuclei are in equilibrium with a single cellular pool of nucleation protein. A minority of the ice nuclei decayed much more slowly than the majority; presumably their nucleation protein was distinct either by virtue of different structure or different subcellular compartmentalization, or because of its presence in a metabolically distinct subpopulation of cells.

Bibliography

Watanabe, N. M., Southworth, M. W., Warren, G. J., & Wolber, P. K. (1990). Rates of assembly and degradation of bacterial ice nuclei. Molecular Microbiology, 4(11), 1871â1879. Portico.

Authors 4

N. M. Watanabe (first)
M. W. Southworth (additional)
G. J. Warren (additional)
P. K. Wolber (additional)

References 39 Referenced 12

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10.1126/science.6356359

Dates

Type	When
Created	18 years, 10 months ago (Oct. 27, 2006, 5:18 p.m.)
Deposited	1 year, 10 months ago (Oct. 22, 2023, 11:46 a.m.)
Indexed	1 year, 10 months ago (Oct. 23, 2023, 1:09 a.m.)
Issued	34 years, 10 months ago (Nov. 1, 1990)
Published	34 years, 10 months ago (Nov. 1, 1990)
Published Online	18 years, 10 months ago (Oct. 27, 2006)
Published Print	34 years, 10 months ago (Nov. 1, 1990)

Funders 0

None

BibTeX

@article{Watanabe_1990, title={Rates of assembly and degradation of bacterial ice nuclei}, volume={4}, ISSN={1365-2958}, url={http://dx.doi.org/10.1111/j.1365-2958.1990.tb02036.x}, DOI={10.1111/j.1365-2958.1990.tb02036.x}, number={11}, journal={Molecular Microbiology}, publisher={Wiley}, author={Watanabe, N. M. and Southworth, M. W. and Warren, G. J. and Wolber, P. K.}, year={1990}, month=nov, pages={1871–1879} }

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