DOI: 10.1111/j.1365-2958.1989.tb00201.x. Cloning and physical characterization of the L‐proline catabolism gene cluster of <i>Aspergillus nidulans</i>

Cloning and physical characterization of the L‐proline catabolism gene cluster of Aspergillus nidulans

10.1111/j.1365-2958.1989.tb00201.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

SummaryThe proline catabolism gene cluster of Aspergillus nidulans was cloned using a ‘brute force’ technique which detects clones hybridizing to restriction fragments overlapping chromosomal rearrangements. A number of deletion mutations and a translocation mutation in the cluster have been physically mapped, and an excellent correlation between the genetic and physical maps was established. Transcripts have been identified and orientated for each of the four genes of the cluster. All are monocistronic by size. All of the transcripts, including that of the regulatory gene prnA, are inducible. Using deletion endpoints and mRNA sizes, approximate gene positions on the physical map have been determined. Finally, the relationship between genetic and physical distance across the cluster has been estimated at 3‐4 kilobases per centiMorgan.

Bibliography

Hull, E. P., Green, P. M., Arst, H. N., & Scazzocchlo, C. (1989). Cloning and physical characterization of the Lâproline catabolism gene cluster of Aspergillus nidulans. Molecular Microbiology, 3(4), 553â559. Portico.

Authors 4

E. P. Hull (first)
P. M. Green (additional)
H. N. Arst (additional)
C. Scazzocchlo (additional)

References 29 Referenced 61

10.1007/BF00330988
10.1038/254026a0
10.1007/BF00268959
{'key': 'e_1_2_1_5_1', 'first-page': '285', 'article-title': 'Regulation of proline transport in Aspergillus nidulans', 'volume': '116', 'author': 'Arst H.N.', 'year': '1980', 'journal-title': 'J Gen Microbiol'} / J Gen Microbiol / Regulation of proline transport in Aspergillus nidulans by Arst H.N. (1980)
10.1017/S0016672300020516
10.1073/pnas.69.6.1408
10.1016/0006-291X(83)91828-4
10.1093/nar/14.18.7505
{'key': 'e_1_2_1_10_1', 'first-page': '325', 'article-title': 'Aspergillus nidulans (n = 8)', 'volume': '4', 'author': 'Clutterbuck A.J.', 'year': '1987', 'journal-title': 'Genetic Maps'} / Genetic Maps / Aspergillus nidulans (n = 8) by Clutterbuck A.J. (1987)
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10.1016/S0021-9258(17)40972-0
10.1002/j.1460-2075.1982.tb01308.x
10.1007/BF00422084
10.1101/SQB.1981.045.01.077
10.1016/0378-1119(88)90200-4
10.1016/B978-0-12-088640-1.50020-3 / Gene Manipulations in Fungi by Green P.M. (1985)
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10.1021/bi00640a033
10.1111/j.1365-2958.1987.tb01933.x
10.1111/j.1475-4754.1982.tb01001.x
10.1016/0003-2697(84)90808-X
10.1073/pnas.79.19.5976
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10.1073/pnas.77.9.5201
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10.1016/0092-8674(80)90434-1

Dates

Type	When
Created	18 years, 10 months ago (Oct. 27, 2006, 4:58 p.m.)
Deposited	1 year, 10 months ago (Oct. 22, 2023, 3:59 a.m.)
Indexed	1 year, 3 months ago (June 4, 2024, 8:52 p.m.)
Issued	36 years, 5 months ago (April 1, 1989)
Published	36 years, 5 months ago (April 1, 1989)
Published Online	18 years, 10 months ago (Oct. 27, 2006)
Published Print	36 years, 5 months ago (April 1, 1989)

Funders 0

None

BibTeX

@article{Hull_1989, title={Cloning and physical characterization of the L‐proline catabolism gene cluster of Aspergillus nidulans}, volume={3}, ISSN={1365-2958}, url={http://dx.doi.org/10.1111/j.1365-2958.1989.tb00201.x}, DOI={10.1111/j.1365-2958.1989.tb00201.x}, number={4}, journal={Molecular Microbiology}, publisher={Wiley}, author={Hull, E. P. and Green, P. M. and Arst, H. N. and Scazzocchlo, C.}, year={1989}, month=apr, pages={553–559} }

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