DOI: 10.1111/j.1365-2958.1989.tb00143.x. Cloning, expression and sequence analysis of the gene encoding the 120kD surface‐exposed protein of <i>Rickettsia rickettsii</i>

Cloning, expression and sequence analysis of the gene encoding the 120kD surface‐exposed protein of Rickettsia rickettsii

10.1111/j.1365-2958.1989.tb00143.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

Summary Rickettsia rickettsii (R strain) genomic DNA was partially digested and cloned into the lambda expression vector gt11 generating a genomic clone bank. Trans‐formant plaques were screened with antisera generated against the 120 kiloDalton protein of R. rickettsii to detect those phage expressing the recombinant protein. The gene encoding the 120kD protein was localized to a 4.3 kilobase Sphl‐BamHl fragment from the recombinant phage and subcioned into pUC18 and pUC19. Full‐length expression of the recombinant protein was achieved with both orientations. The gene and flanking regions were sequenced. The p120 gene consists of 3900 base pairs coding for 1300 amino acids. A distinguishable promoter region was not identified, although there are several 5′ sequences that resemble classical prokaryotic promoters. Downstream of the termination codon for this gene lies a 726 base pair open reading frame on the opposite strand with the potential to encode a protein of approximately 27 kD. The identity of this putative gene product is unknown. The two open reading frames are separated by a 106 base pair intergenic region that consists of a stretch of dyad symmetry resembling rho‐independent transcriptional terminators.

Bibliography

Gilmore, R. D., Joste, N., & McDonald, G. A. (1989). Cloning, expression and sequence analysis of the gene encoding the 120kD surfaceâexposed protein of Rickettsia rickettsii. Molecular Microbiology, 3(11), 1579â1586. Portico.

Authors 3

R. D. Gilmore (first)
N. Joste (additional)
G. A. McDonald (additional)

References 28 Referenced 57

10.1128/jb.94.1.260-262.1967 / J Bacteriol / Details of the ultrastructure of Rickettsia prowazeki grown in the chick yolk sac by Anacker R.L (1967)
10.1093/infdis/151.6.1052
10.1128/iai.51.2.653-660.1986 / Infect Immun / Antigenic heterogeneity in high‐ and low‐virulence strains of Rickettsia rickettsii. revealed by monoclonal antibodies by Anacker R.L. (1986)
10.1128/JCM.25.1.167-171.1987
10.1128/iai.55.3.825-827.1987 / Infect Immun / Neutralizing activity of monoclonal antibodies to heat‐sensitive and heat‐resistant epitopes of Rickettsia rickettsii surface proteins by Anacker R.L. (1987)
10.1128/iai.44.3.559-564.1984 / Infect Immun / Biochemical and immunochemical analysis of Rickettsia rickettsii strains of various degrees of virulence by Anacker R.L (1984)
10.1128/jb.169.6.2385-2390.1987
10.1093/nar/12.10.4411
{'key': 'e_1_2_1_10_1', 'first-page': '187', 'article-title': 'Rocky Mountain spotted fever–United States. 1983', 'volume': '33', 'author': 'Centers for Disease Control', 'year': '1984', 'journal-title': 'MMWR'} / MMWR / Rocky Mountain spotted fever–United States. 1983 by Centers for Disease Control (1984)
{'key': 'e_1_2_1_11_1', 'volume-title': 'Proteins. Structures and Molecular Principles.', 'author': 'Creighton T.E.', 'year': '1984'} / Proteins. Structures and Molecular Principles. by Creighton T.E. (1984)
10.1093/nar/11.8.2237
Hayes S.F. andBurgdorfer W.(1982)Reactivation ofRickettsia rickettsiiInDermacentor andersoniticks: an ultrastructural analysis infect Immun37:779–786. (10.1128/iai.37.2.779-785.1982)
10.1111/j.1462-5822.2007.00901.x
10.1016/S0021-9258(18)47994-X / J Biol Chem / The primary structure of a procaryotic glycoprotein. Cloning and sequencing of the cell surface glycoprotein gene of halobacteria by Lechner J. (1987)
10.1111/j.1475-4754.1982.tb01001.x
10.1126/science.3099387
10.1093/infdis/158.1.228
10.1016/S0021-9258(17)33647-5
10.1128/iai.21.3.866-873.1978 / Infect Immun / Surface proteins of typhus and spotted fever group rickettsiae by Osterman J.V. (1978)
10.1128/am.28.4.713-716.1974 / Appl Microbiol / Ultrastructure of the surface of Rickettsia prowazeki and Rickettsia prowazeki and Rickettsai akari by Palmer E.L. (1974)
10.1128/JB.118.3.1158-1166.1974
10.1073/pnas.72.9.3310
10.1073/pnas.74.12.5463
10.1128/jb.170.7.2891-2897.1988
10.1073/pnas.76.9.4350 / Proc NatI Acad Sci USA / Electrophoretic transfer of proteins from potyacrylamide gels to nitrocellulose sheets: procedure and some applications by Towbin H. (1979)
10.1073/pnas.80.18.5470
10.1128/iai.52.1.120-127.1986 / Infect Immun / Humoral immune response to Rocky Mountain spotted fever in experimentally infected guinea pigs: immunoprecipitation of lactoperoxidase 125l‐labeled proteins and detection of soluble antigens of Rickettsia rickettsii by Williams J.C. (1986)
10.1128/jb.169.8.3564-3572.1987

Dates

Type	When
Created	18 years, 10 months ago (Oct. 27, 2006, 4:53 p.m.)
Deposited	1 year, 10 months ago (Oct. 22, 2023, 12:16 a.m.)
Indexed	1 year, 4 months ago (April 7, 2024, 11:52 a.m.)
Issued	35 years, 10 months ago (Nov. 1, 1989)
Published	35 years, 10 months ago (Nov. 1, 1989)
Published Online	18 years, 10 months ago (Oct. 27, 2006)
Published Print	35 years, 10 months ago (Nov. 1, 1989)

Funders 0

None

BibTeX

@article{Gilmore_1989, title={Cloning, expression and sequence analysis of the gene encoding the 120kD surface‐exposed protein of Rickettsia rickettsii}, volume={3}, ISSN={1365-2958}, url={http://dx.doi.org/10.1111/j.1365-2958.1989.tb00143.x}, DOI={10.1111/j.1365-2958.1989.tb00143.x}, number={11}, journal={Molecular Microbiology}, publisher={Wiley}, author={Gilmore, R. D. and Joste, N. and McDonald, G. A.}, year={1989}, month=nov, pages={1579–1586} }

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