DOI: 10.1111/j.1365-294x.1994.tb00112.x. Transduction of a freshwater microbial community by a new <i>Pseudomonas aeruginosa</i> generalized transducing phage, UT1

Transduction of a freshwater microbial community by a new Pseudomonas aeruginosa generalized transducing phage, UT1

10.1111/j.1365-294x.1994.tb00112.x

Crossref journal-article

Wiley

Molecular Ecology (311)

Abstract

AbstractA pseudolysogenic, generalized transducing bacteriophage, UT1, isolated from a natural freshwater habitat, is capable of mediating the transfer of both chromosomal andplasmid DNA between strains of Pseudomonas aeruginosa. Several chromosomal alleles from three different P. aeruginosa strains were found to transduce at frequencies from 10‐8 to 10‐10 transductants per PFU at multiplicities of infection (MOD between 0.1 and 1. Transduction frequencies of certain alleles increased up to 1000‐fold as MOIs were decreased to 0.01. UT1 is also capable of transducing plasmid DNA to indigenous populations of microorganisms in natural lake‐water environments. Data obtained in this study suggest that environmentally endemic bacteriophages such as UT1 are formidable transducers of naturally occurring microbial communities. It should be possible to develop model systems to test transduction in freshwater environments using components derived exclusively from these environments.

Bibliography

RIPP, S., OGUNSEITAN, O. A., & MILLER, R. V. (1994). Transduction of a freshwater microbial community by a new Pseudomonas aeruginosa generalized transducing phage, UT1. Molecular Ecology, 3(2), 121â126. Portico.

Authors 3

S. RIPP (first)
O. A. OGUNSEITAN (additional)
R. V. MILLER (additional)

References 29 Referenced 44

10.1128/aem.36.3.492-499.1978 / Applied and Environmental Microbiology / Incidence of Vibrio parahaemolyticus bacteriophages and other Vibrio bacteriophages in marine samples by Baross JA (1978)
10.1016/S0147-619X(80)90029-3
{'key': 'e_1_2_1_4_1', 'volume-title': "The Shorter Bergey's Manual of Determinative Bacteriology", 'author': 'Holt JG', 'year': '1977'} / The Shorter Bergey's Manual of Determinative Bacteriology by Holt JG (1977)
10.1128/jb.89.5.1286-1290.1965 / Journal of Bacteriology / Transduction of Staphylococcus aureus to tetracycline resistance in vivo by Jarolmen H. (1965)
KidambiSP RippS. MillerRV(1993)Evidence for phage‐mediated transfer amongPseudomonas aeruginosaon the phylloplane.Applied and Environmental Microbiology submitted.
{'key': 'e_1_2_1_7_1', 'first-page': '73', 'volume-title': 'Gene Transfer in the Environment', 'author': 'Kokjohn TA', 'year': '1989'} / Gene Transfer in the Environment by Kokjohn TA (1989)
{'key': 'e_1_2_1_8_1', 'first-page': '54', 'volume-title': 'Release of Genetically Engineered and Other Microorganisms', 'author': 'Kokjohn TA', 'year': '1992'} / Release of Genetically Engineered and Other Microorganisms by Kokjohn TA (1992)
{'key': 'e_1_2_1_9_1', 'first-page': '221', 'volume-title': 'Microbiology‐1978', 'author': 'Korfhagen TT', 'year': '1978'} / Microbiology‐1978 by Korfhagen TT (1978)
10.1007/BF00332784
{'key': 'e_1_2_1_11_1', 'first-page': '229', 'volume-title': 'Microbial Ecology: Principles, Methods, and Applications', 'author': 'Miller RV', 'year': '1992'} / Microbial Ecology: Principles, Methods, and Applications by Miller RV (1992)
{'key': 'e_1_2_1_12_1', 'first-page': '252', 'volume-title': 'Pseudomonas: Biotransformation, Pathogenesis, and Evolving Biotechnology', 'author': 'Miller RV', 'year': '1990'} / Pseudomonas: Biotransformation, Pathogenesis, and Evolving Biotechnology by Miller RV (1990)
10.1128/jb.134.3.875-883.1978 / Journal of Bacteriology / Characterization of Pseudomonas aeruginosa mutants deficient in the establishment of lysogeny by Miller RV (1978)
10.1128/jvi.22.3.844-847.1977 / Journal of Virology / Prophage F116: evidence for extrachromosomal location in Pseudomonas aeruginosa strain PAO by Miller RV (1976)
10.1016/0042-6822(74)90466-8
10.1007/978-3-642-77450-8_7
10.1016/B978-0-08-042000-4.50017-6
10.1128/jb.139.1.137-140.1979 / Journal of Bacteriology / Transduction of Pseudomonas aeruginosa with a mutant of bacteriophage E79 by Morgan AF (1979)
10.1128/aem.36.5.724-730.1978 / Applied and Environmental Microbiology / Frequency of F116‐mediated transduction of Pseudomonas aeruginosa in a freshwater environment by Morrison WD (1978)
10.1016/0022-2836(86)90360-8
10.1084/jem.125.1.45
10.1007/BF02012098
10.1128/aem.58.6.2046-2052.1992 / Applied and Environmental Microbiology / Application of DNA probes to the analysis of bacteriophage distribution in the environment by Ogunseitan OA (1992)
10.1128/jb.82.1.135-141.1961 / Journal of Bacteriology / Isolation and preliminary characterization of bacteriophages of Bacillus subtilis by Romig WR (1961)
{'key': 'e_1_2_1_25_1', 'volume-title': 'Molecular Cloning. A Laboratory Manual', 'author': 'Sambrook J.', 'year': '1989'} / Molecular Cloning. A Laboratory Manual by Sambrook J. (1989)
10.1128/aem.56.1.140-145.1990 / Applied and Environmental Microbiology / Transduction of linked chromosomal genes between Pseudomonas aeruginosa during incubation in situ in a freshwater habitat by Saye DJ (1990)
10.1128/aem.53.5.987-995.1987 / Applied and Environmental Microbiology / Potential for transduction of plasmids in a natural freshwater environment: effect of donor concentration and a natural microbial community on transduction in Pseudomonas aeruginosa by Saye DJ (1987)
{'key': 'e_1_2_1_28_1', 'first-page': '165', 'volume-title': 'Gene Transfer in the Environment', 'author': 'Stotzky G.', 'year': '1989'} / Gene Transfer in the Environment by Stotzky G. (1989)
10.1128/aem.55.3.661-665.1989 / Applied and Environmental Microbiology / Use of a biotinylated DNA probe to detect bacteria transduced by bacteriophage PI in soil by Zeph LR (1989)
10.1128/aem.54.7.1731-1737.1988 / Applied and Environmental Microbiology / Transduction of Escherichia coli by bacteriophage PI in soil by Zeph LR (1988)

Dates

Type	When
Created	17 years, 4 months ago (April 14, 2008, 4:15 a.m.)
Deposited	1 year, 10 months ago (Oct. 23, 2023, 9:08 p.m.)
Indexed	3 months, 2 weeks ago (May 18, 2025, 10:25 a.m.)
Issued	31 years, 5 months ago (April 1, 1994)
Published	31 years, 5 months ago (April 1, 1994)
Published Online	16 years, 10 months ago (Oct. 9, 2008)
Published Print	31 years, 5 months ago (April 1, 1994)

Funders 0

None

BibTeX

@article{RIPP_1994, title={Transduction of a freshwater microbial community by a new Pseudomonas aeruginosa generalized transducing phage, UT1}, volume={3}, ISSN={1365-294X}, url={http://dx.doi.org/10.1111/j.1365-294x.1994.tb00112.x}, DOI={10.1111/j.1365-294x.1994.tb00112.x}, number={2}, journal={Molecular Ecology}, publisher={Wiley}, author={RIPP, S. and OGUNSEITAN, O. A. and MILLER, R. V.}, year={1994}, month=apr, pages={121–126} }

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