DOI: 10.1111/j.1365-2826.1989.tb00122.x. Melatonin Receptor Sites in the Syrian Hamster Brain and Pituitary. Localization and Characterization Using [<sup>125</sup>|]lodomelatonin*

Melatonin Receptor Sites in the Syrian Hamster Brain and Pituitary. Localization and Characterization Using [125|]lodomelatonin*

10.1111/j.1365-2826.1989.tb00122.x

Crossref journal-article

Wiley

Journal of Neuroendocrinology (311)

Abstract

AbstractA high‐affinity, discretely localized melatonin receptor has been characterized and mapped within the brain and pituitary of the Syrian hamster using the high specific activity ligand [125|]iodomelatonin and a combination of in vitro autoradiography and membrane homogenate receptor assays. Specific binding of radioligand was found in regions of the epithalamus and hypothalamus in the brain and the pars tuberalis of the pituitary. Excitatory amino‐acid lesions destroyed [125|]iodomelatonin binding within the brain, demonstrating that binding sites are located on neurons. Analysis of [125|]iodomelatonin binding to membrane homogenates of the pars tuberalis revealed a linear relationship between specific ligand binding and the amount of tissue. The time‐course of specific binding at 37°C reached equilibrium after 30 min and remained stable thereafter. The addition of increasing concentrations of [125|]iodomelatonin alone and in the presence of 1 μM melatonin showed that specific binding reached equilibrium at 80 to 100 pM. Analysis of the saturation isotherm using a one‐site binding model was consistent with a single receptor site with a Kd of 29.3 (±5.9 SEM) pM and Bmax of 2.54 (±0.19 SEM) fmol/mg protein.

Bibliography

Williams, L. M., Morgan, P. J., Hastings, M. H., Lawson, W., Davidson, G., & Howell, H. E. (1989). Melatonin Receptor Sites in the Syrian Hamster Brain and Pituitary. Localization and Characterization Using [125|]lodomelatonin*. Journal of Neuroendocrinology, 1(5), 315â320. Portico.

Authors 6

L. M. Williams (first)
P. J. Morgan (additional)
M. H. Hastings (additional)
W. Lawson (additional)
G. Davidson (additional)
H. E. Howell (additional)

References 43 Referenced 96

{'key': 'e_1_2_1_2_2', 'first-page': '266', 'volume-title': 'Oxford reviews of reproductive biology', 'author': 'Arendt J', 'year': '1986'} / Oxford reviews of reproductive biology by Arendt J (1986)
{'key': 'e_1_2_1_3_2', 'volume-title': 'Neuroendocrinology', 'author': 'Wurtman RG', 'year': '1983'} / Neuroendocrinology by Wurtman RG (1983)
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Dates

Type	When
Created	18 years, 11 months ago (Sept. 29, 2006, 6:04 a.m.)
Deposited	1 year, 10 months ago (Oct. 22, 2023, 1:34 a.m.)
Indexed	11 months, 3 weeks ago (Sept. 7, 2024, 7:55 p.m.)
Issued	35 years, 11 months ago (Oct. 1, 1989)
Published	35 years, 11 months ago (Oct. 1, 1989)
Published Online	18 years, 11 months ago (Sept. 29, 2006)
Published Print	35 years, 11 months ago (Oct. 1, 1989)

Funders 0

None

BibTeX

@article{Williams_1989, title={Melatonin Receptor Sites in the Syrian Hamster Brain and Pituitary. Localization and Characterization Using [125|]lodomelatonin*}, volume={1}, ISSN={1365-2826}, url={http://dx.doi.org/10.1111/j.1365-2826.1989.tb00122.x}, DOI={10.1111/j.1365-2826.1989.tb00122.x}, number={5}, journal={Journal of Neuroendocrinology}, publisher={Wiley}, author={Williams, L. M. and Morgan, P. J. and Hastings, M. H. and Lawson, W. and Davidson, G. and Howell, H. E.}, year={1989}, month=oct, pages={315–320} }

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