DOI: 10.1111/j.1365-2818.1992.tb03266.x. High‐resolution imaging of chromosome‐related structures by atomic force microscopy

High‐resolution imaging of chromosome‐related structures by atomic force microscopy

10.1111/j.1365-2818.1992.tb03266.x

Crossref journal-article

Wiley

Journal of Microscopy (311)

Abstract

SUMMARYAn atomic force microscope (AFM) was combined with a conventional optical microscope. The optical microscope proved to be very convenient for locating objects of interest. In addition, the high‐resolution AFM image can be compared directly with the traditional optical image. The instrument was used to study chromosome structures. High‐resolution chromosome images revealed details of the 30‐nm chromatide structure, confirming earlier electron microscopic observations. Chromosomes treated with trypsin revealed a banding pattern in height which is very similar to the optical image observed after staining with Giemsa. Furthermore, it is shown that the AFM can be used to locate DNA probes on in situ hybridized chromosomes. Images of the synaptonemal complex isolated from rat spermatocytes revealed details that improve the understanding of the three‐dimensional structure of this protein.

Bibliography

Grooth, B. G. D., & Putman, C. A. J. (1992). Highâresolution imaging of chromosomeârelated structures by atomic force microscopy. Journal of Microscopy, 168(3), 239â247. Portico.

Authors 2

Bart G. De Grooth (first)
Constant A. J. Putman (additional)

References 27 Referenced 59

10.1116/1.576520
{'key': 'e_1_2_1_3_1', 'first-page': '51', 'volume-title': 'Chromosomes and Chromatin', 'author': 'Allen T.D.', 'year': '1988'} / Chromosomes and Chromatin by Allen T.D. (1988)
10.1103/PhysRevLett.49.57
10.1063/1.1139196
10.1103/PhysRevLett.56.930
10.1016/1047-8477(90)90098-W / J. Struct. Biol. / Imaging cells with the atomic force microscope by Butt H.‐J. (1991)
10.1021/bi00116a005
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10.1016/S0006-3495(90)82489-9
10.1146/annurev.ge.09.120175.000515
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10.1126/science.256.5060.1180
{'key': 'e_1_2_1_16_1', 'first-page': '307', 'article-title': 'Structure and composition of synaptonemal complexes isolated from rat spermatocytes', 'volume': '36', 'author': 'Heyting C.', 'year': '1985', 'journal-title': 'Eur. J. Cell. Biol.'} / Eur. J. Cell. Biol. / Structure and composition of synaptonemal complexes isolated from rat spermatocytes by Heyting C. (1985)
10.1126/science.1910206
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10.1063/1.1143303
10.1016/S0140-6736(71)90287-X
10.1063/1.101024
10.1093/nar/19.12.3237

Dates

Type	When
Created	14 years ago (Aug. 2, 2011, 11:53 a.m.)
Deposited	1 year, 10 months ago (Oct. 23, 2023, 7:33 p.m.)
Indexed	1 year, 2 months ago (June 17, 2024, 4:37 p.m.)
Issued	32 years, 8 months ago (Dec. 1, 1992)
Published	32 years, 8 months ago (Dec. 1, 1992)
Published Online	14 years ago (Aug. 2, 2011)
Published Print	32 years, 8 months ago (Dec. 1, 1992)

Funders 0

None

BibTeX

@article{Grooth_1992, title={High‐resolution imaging of chromosome‐related structures by atomic force microscopy}, volume={168}, ISSN={1365-2818}, url={http://dx.doi.org/10.1111/j.1365-2818.1992.tb03266.x}, DOI={10.1111/j.1365-2818.1992.tb03266.x}, number={3}, journal={Journal of Microscopy}, publisher={Wiley}, author={Grooth, Bart G. De and Putman, Constant A. J.}, year={1992}, month=dec, pages={239–247} }

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