DOI: 10.1111/j.1365-2818.1992.tb03217.x. A 360° single‐axis tilt stage for the high‐voltage electron microscope

A 360° single‐axis tilt stage for the high‐voltage electron microscope

10.1111/j.1365-2818.1992.tb03217.x

Crossref journal-article

Wiley

Journal of Microscopy (311)

Abstract

SUMMARYA new type of specimen stage that permits more than 180° of tilting about the axis of a side‐entry rod has been developed for a high‐voltage electron microscope (HVEM). Roughly cylindrical specimens, with radial dimensions of less than a few micrometres, that can be mounted on the tip of a microneedle or micropipette are applicable. For glass micropipettes, the energy of the 1‐MeV beam of the HVEM is sufficient to image specimens through both walls. The stage employs a spindle mechanism that holds these needles or micropipettes coaxial with the tilt axis, allowing the specimen to be rotated without restriction. This arrangement, along with the cylindrical form of the specimen, is an important development for single‐axis tomography, because it permits a complete 180° set of projections to be recorded. The angular accuracy of the stage was demonstrated to be within ±0.20°, with a cumulative error of less than 1.0° over a 180° span.The new stage was tested using puffball spores mounted on a micropipette. A 180° tilt series was recorded and processed to yield a tomographic three‐dimensional reconstruction which was displayed both as a cross‐sectional view perpendicular to the tilt axis, and as a shaded surface viewed from different directions. The same computations were repeated using subsets of the tilt series to assess the effect of various amounts of missing information. Visual inspection of a selected cross‐section from these reconstructions indicated that limiting the angular range to 160° produced results nearly as good as the full data set. Limiting the range to 140°, however, produced a noticeable geometric distortion, which became increasingly severe with ranges of 120° and 100°.

Bibliography

Barnard, D. P., Turner, J. N., Frank, J., & McEwen, B. F. (1992). A 360Â° singleâaxis tilt stage for the highâvoltage electron microscope. Journal of Microscopy, 167(1), 39â48. Portico.

Authors 4

David P. Barnard (first)
James N. Turner (additional)
Joachim Frank (additional)
Bruce F. McEwen (additional)

References 34 Referenced 48

10.1016/0022-2836(86)90231-7
10.1083/jcb.105.1.77
10.1111/j.1365-2818.1987.tb01313.x
{'key': 'e_1_2_1_5_1', 'first-page': '159', 'article-title': 'Restoration of direct Fourier three‐dimensional reconstructions of crystalline specimens by the method of convex sets', 'volume': '145', 'author': 'Carazo J.M.', 'year': '1987', 'journal-title': 'J. Microsc.'} / J. Microsc. / Restoration of direct Fourier three‐dimensional reconstructions of crystalline specimens by the method of convex sets by Carazo J.M. (1987)
10.1111/j.1365-2818.1984.tb00502.x
10.1098/rspa.1970.0119
{'key': 'e_1_2_1_8_1', 'first-page': '60', 'volume-title': 'Single‐Channel Recording', 'author': 'Corey D.P.', 'year': '1983'} / Single‐Channel Recording by Corey D.P. (1983)
10.1002/jemt.1060060210
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{'key': 'e_1_2_1_12_1', 'first-page': '253', 'article-title': 'Early atherosclerosis with white carneu pigeon III. Lipid accumulation in nascent foam cells', 'volume': '128', 'author': 'Jerome W.G.', 'year': '1987', 'journal-title': 'Am. J. Pathol.'} / Am. J. Pathol. / Early atherosclerosis with white carneu pigeon III. Lipid accumulation in nascent foam cells by Jerome W.G. (1987)
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{'key': 'e_1_2_1_14_1', 'first-page': '24a', 'article-title': 'Spatial relations between collagen and mineral in calcifying tendon determined by high voltage electron microscopy and tomographic 3‐D reconstruction', 'volume': '111', 'author': 'Landis W.J.', 'year': '1990', 'journal-title': 'J. Cell Biol.'} / J. Cell Biol. / Spatial relations between collagen and mineral in calcifying tendon determined by high voltage electron microscopy and tomographic 3‐D reconstruction by Landis W.J. (1990)
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{'key': 'e_1_2_1_19_1', 'first-page': '522', 'article-title': 'Tomographic three‐dimensional reconstruction of patch‐clamped membranes imaged with the high‐voltage electron microscope', 'volume': '1', 'author': 'McEwen B.F.', 'year': '1990', 'journal-title': 'Proc. XII Intl. Congr. Elee. Microsc.'} / Proc. XII Intl. Congr. Elee. Microsc. / Tomographic three‐dimensional reconstruction of patch‐clamped membranes imaged with the high‐voltage electron microscope by McEwen B.F. (1990)
{'key': 'e_1_2_1_20_1', 'first-page': '129', 'article-title': 'Modeling Balbiani ring gene transcription with electron microscope tomography', 'volume': '35', 'author': 'Olins A.L.', 'year': '1984', 'journal-title': 'Eur. J. Cell Biol.'} / Eur. J. Cell Biol. / Modeling Balbiani ring gene transcription with electron microscope tomography by Olins A.L. (1984)
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{'key': 'e_1_2_1_30_1', 'first-page': '337', 'article-title': 'High precision tilt stage for the high‐voltage electron microscope', 'volume': '26', 'author': 'Turner J.N.', 'year': '1988', 'journal-title': 'Ultramicroscopy'} / Ultramicroscopy / High precision tilt stage for the high‐voltage electron microscope by Turner J.N. (1988)
{'key': 'e_1_2_1_31_1', 'first-page': '93', 'article-title': 'Three dimensional imaging at high resolution: high‐voltage electron microscopy', 'volume': '13', 'author': 'Turner J.N.', 'year': '1990', 'journal-title': 'J. Histotechnol.'} / J. Histotechnol. / Three dimensional imaging at high resolution: high‐voltage electron microscopy by Turner J.N. (1990)
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{'key': 'e_1_2_1_35_1', 'first-page': '520', 'article-title': 'On the use of POCS for restoring the “missing wedge” in electron tomography', 'volume': '1', 'author': 'Zhang N.Y.', 'year': '1990', 'journal-title': 'Proc. XII Int. Cong. Electron Microsc, Seattle'} / Proc. XII Int. Cong. Electron Microsc, Seattle / On the use of POCS for restoring the “missing wedge” in electron tomography by Zhang N.Y. (1990)

Dates

Type	When
Created	14 years ago (Aug. 2, 2011, 7:50 a.m.)
Deposited	1 year, 10 months ago (Oct. 15, 2023, 2:20 a.m.)
Indexed	1 day, 12 hours ago (Aug. 21, 2025, 12:46 p.m.)
Issued	33 years, 1 month ago (July 1, 1992)
Published	33 years, 1 month ago (July 1, 1992)
Published Online	14 years ago (Aug. 2, 2011)
Published Print	33 years, 1 month ago (July 1, 1992)

Funders 0

None

BibTeX

@article{Barnard_1992, title={A 360° single‐axis tilt stage for the high‐voltage electron microscope}, volume={167}, ISSN={1365-2818}, url={http://dx.doi.org/10.1111/j.1365-2818.1992.tb03217.x}, DOI={10.1111/j.1365-2818.1992.tb03217.x}, number={1}, journal={Journal of Microscopy}, publisher={Wiley}, author={Barnard, David P. and Turner, James N. and Frank, Joachim and McEwen, Bruce F.}, year={1992}, month=jul, pages={39–48} }

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