DOI: 10.1111/j.1365-2818.1991.tb03155.x. Cryosectioning of plant material frozen at high pressure

Cryosectioning of plant material frozen at high pressure

10.1111/j.1365-2818.1991.tb03155.x

Crossref journal-article

Wiley

Journal of Microscopy (311)

Abstract

SUMMARYLow‐temperature electron microscopy combined with electron diffraction allows determination of the state of structural preservation as well as that of the solidified cellular water in frozen‐hydrated sections. Apple leaves frozen at high pressure and cryosectioned with glass knives covered with a very thin layer of frozen water exhibit a minimum of artefacts and are thin enough for structural analysis. The Zeiss 902 EM energy‐filtering microscope helps to improve the poor contrast of frozen‐hydrated sections by imaging in the zero‐loss mode. All sections analysed showed the electron diffraction pattern of vitreous water. This is ideal: the character of the liquid phase is preserved. A very high yield of good cryosections is achieved because the samples are vitrified and the friction on the glass knife surface is reduced by a thin ice layer.

Bibliography

Michel, M., Hillmann, T., & MÃ¼ller, M. (1991). Cryosectioning of plant material frozen at high pressure. Journal of Microscopy, 163(1), 3â18. Portico.

Authors 3

M. Michel (first)
T. Hillmann (additional)
M. Müller (additional)

References 42 Referenced 66

10.1111/j.1365-2818.1983.tb04714.x
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{'key': 'e_1_2_1_7_1', 'first-page': '1407', 'volume-title': 'Proc. Eighth Eur. Congr. on Electron Microscopy', 'author': 'Dubochet J.', 'year': '1984'} / Proc. Eighth Eur. Congr. on Electron Microscopy by Dubochet J. (1984)
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Dates

Type	When
Created	14 years ago (Aug. 2, 2011, 10:36 a.m.)
Deposited	1 year, 9 months ago (Oct. 22, 2023, 10:23 p.m.)
Indexed	4 weeks, 1 day ago (July 22, 2025, 6:59 a.m.)
Issued	34 years, 1 month ago (July 1, 1991)
Published	34 years, 1 month ago (July 1, 1991)
Published Online	14 years ago (Aug. 2, 2011)
Published Print	34 years, 1 month ago (July 1, 1991)

Funders 0

None

BibTeX

@article{Michel_1991, title={Cryosectioning of plant material frozen at high pressure}, volume={163}, ISSN={1365-2818}, url={http://dx.doi.org/10.1111/j.1365-2818.1991.tb03155.x}, DOI={10.1111/j.1365-2818.1991.tb03155.x}, number={1}, journal={Journal of Microscopy}, publisher={Wiley}, author={Michel, M. and Hillmann, T. and Müller, M.}, year={1991}, month=jul, pages={3–18} }

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