DOI: 10.1111/j.1365-2818.1991.tb03081.x. Radiation damage in dry and frozen hydrated organic material

Radiation damage in dry and frozen hydrated organic material

10.1111/j.1365-2818.1991.tb03081.x

Crossref journal-article

Wiley

Journal of Microscopy (311)

Abstract

SUMMARYElectron‐induced radiation damage can cause errors in interpreting electron micrographs. Radiation damage is distinguished from contamination (polymerization of hydrocarbons) and etching (radiolysis in the presence of water), both of which can be controlled by a proper specimen environment in the microscope. While temperature has little effect on the primary interactions of fast electrons with matter, most secondary radiation‐damage processes are temperature dependent. Because damage mechanisms differ so greatly among materials, there is no simple factor by which specimen stability is improved as a function of temperature (some cases improve fivefold, others improve 100‐fold). While some specimens are stable to almost arbitrarily high doses, some tests reveal damage at 1 e/nm2. This paper surveys damage rates and temperature dependencies of various materials as a guide for future electron microscopic studies of organic specimens.

Authors 1

M. K. Lamvik (first)

References 66 Referenced 38

{'key': 'e_1_2_1_2_1', 'first-page': '1115', 'article-title': 'The elementary composition of organic objects after electron irradiation', 'volume': '14', 'author': 'Bahr G.F.', 'year': '1965', 'journal-title': 'Lab. Invest.'} / Lab. Invest. / The elementary composition of organic objects after electron irradiation by Bahr G.F. (1965)
{'key': 'e_1_2_1_3_1', 'first-page': '955', 'article-title': 'The determination of the dry mass in populations of isolated particles', 'volume': '14', 'author': 'Bahr G.F.', 'year': '1965', 'journal-title': 'Lab. Invest.'} / Lab. Invest. / The determination of the dry mass in populations of isolated particles by Bahr G.F. (1965)
10.1016/0022-2836(88)90288-4
10.1016/0304-3991(86)90095-1
10.1038/178305a0
{'key': 'e_1_2_1_7_1', 'first-page': '183', 'volume-title': 'Radiation Effects on Organic Materials', 'author': 'Bopp C.D.', 'year': '1963'} / Radiation Effects on Organic Materials by Bopp C.D. (1963)
10.1007/BF01333152
10.1111/j.1365-2818.1986.tb02809.x
{'key': 'e_1_2_1_10_1', 'first-page': '408', 'volume-title': 'Proc. 12th Int. Cong. on Electron Microscopy', 'author': 'Ciliax B.J.', 'year': '1990'} / Proc. 12th Int. Cong. on Electron Microscopy by Ciliax B.J. (1990)
10.1007/978-1-4684-6247-0
10.1111/j.1365-2818.1972.tb01059.x
10.1016/0304-3991(83)90003-7
10.1016/0304-3991(82)90187-5
10.1111/j.1365-2818.1982.tb04625.x
{'key': 'e_1_2_1_16_1', 'first-page': '271', 'article-title': 'Electron irradiation effects in the EPMA quantitation of organic specimens', 'volume': '2', 'author': 'Edie J.W.', 'year': '1980', 'journal-title': 'Scanning Electron Microsc.'} / Scanning Electron Microsc. / Electron irradiation effects in the EPMA quantitation of organic specimens by Edie J.W. (1980)
10.1111/j.1365-2818.1982.tb00360.x
10.1016/S0304-3991(78)80037-0
10.1111/j.1365-2818.1981.tb01267.x
10.1016/0304-3991(84)90221-3
10.1111/j.1365-2818.1975.tb03896.x
10.1111/j.1365-2818.1978.tb01160.x
10.1080/14786437108217051
10.1111/j.1365-2818.1986.tb02726.x
10.1111/j.1365-2818.1974.tb03927.x
{'key': 'e_1_2_1_26_1', 'first-page': '116', 'article-title': 'Die Objektverschmutzung im Elektronenmikroskop und das Problem der Strahlenschädigung durch Kohlenstoffabbau', 'volume': '15', 'author': 'Heide H.‐G.', 'year': '1963', 'journal-title': 'Z. Angew. Phys.'} / Z. Angew. Phys. / Die Objektverschmutzung im Elektronenmikroskop und das Problem der Strahlenschädigung durch Kohlenstoffabbau by Heide H.‐G. (1963)
{'key': 'e_1_2_1_27_1', 'first-page': '1134', 'article-title': 'Contamination and irradiation effects and their dependence on the composition of residual gases in the electron microscope', 'volume': '14', 'author': 'Heide H.‐G.', 'year': '1965', 'journal-title': 'Lab. Invest.'} / Lab. Invest. / Contamination and irradiation effects and their dependence on the composition of residual gases in the electron microscope by Heide H.‐G. (1965)
10.1016/0304-3991(82)90178-4
10.1016/0304-3991(84)90095-0
10.1016/0304-3991(85)90070-1
{'key': 'e_1_2_1_31_1', 'first-page': '400', 'volume-title': 'Quantitative Electron Microscopy', 'author': 'Hobbs L.W.', 'year': '1984'} / Quantitative Electron Microscopy by Hobbs L.W. (1984)
10.1016/0304-3991(80)90049-2
{'key': 'e_1_2_1_33_1', 'first-page': '1', 'volume-title': 'Principles and Techniques of Electron Microscopy: Biological Applications', 'author': 'Isaacson M.S.', 'year': '1977'} / Principles and Techniques of Electron Microscopy: Biological Applications by Isaacson M.S. (1977)
{'key': 'e_1_2_1_34_1', 'first-page': '61', 'volume-title': 'Proc. 9th Int. Cong. on Electron Microscopy', 'author': 'Isaacson M.', 'year': '1978'} / Proc. 9th Int. Cong. on Electron Microscopy by Isaacson M. (1978)
10.1111/j.1365-2818.1984.tb02544.x
10.1016/0022-2836(80)90382-4
10.1016/0022-2836(78)90108-0
{'key': 'e_1_2_1_38_1', 'first-page': '816', 'volume-title': 'Proc. 47th Ann. Meet. EMSA.', 'author': 'Lamvik M.K.', 'year': '1989'} / Proc. 47th Ann. Meet. EMSA. by Lamvik M.K. (1989)
10.1002/jemt.1060080403
{'key': 'e_1_2_1_40_1', 'first-page': '636', 'volume-title': 'Proc. 46th Ann. Meet. EMSA.', 'author': 'Lamvik M.K.', 'year': '1988'} / Proc. 46th Ann. Meet. EMSA. by Lamvik M.K. (1988)
10.1016/0304-3991(89)90017-X
{'key': 'e_1_2_1_42_1', 'first-page': '271', 'volume-title': 'Scanning Microscopy', 'author': 'Lamvik M.K.', 'year': '1989'} / Scanning Microscopy by Lamvik M.K. (1989)
10.1111/j.1365-2818.1987.tb02867.x
10.1038/301332a0
10.1016/0304-3991(84)90041-X
{'key': 'e_1_2_1_46_1', 'first-page': '412', 'volume-title': 'Proc. 40th Ann. Meet. EMSA.', 'author': 'Leapman R.D.', 'year': '1982'} / Proc. 40th Ann. Meet. EMSA. by Leapman R.D. (1982)
10.1016/0022-2836(83)90073-6
10.1111/j.1365-2818.1983.tb04590.x
10.2307/3574071
10.1038/133911b0
{'key': 'e_1_2_1_51_1', 'volume-title': 'Early History of the Electron Microscope', 'author': 'Marton L.', 'year': '1968'} / Early History of the Electron Microscope by Marton L. (1968)
10.1021/ja00521a086
10.1016/S0304-3991(85)80010-3
10.1021/j100326a061
{'key': 'e_1_2_1_55_1', 'first-page': '1082', 'article-title': 'Irradiation changes in organic and inorganic objects', 'volume': '14', 'author': 'Reimer L.', 'year': '1965', 'journal-title': 'Lab. Invest.'} / Lab. Invest. / Irradiation changes in organic and inorganic objects by Reimer L. (1965)
10.1016/0304-3991(82)90039-0
10.1016/0304-3991(76)90049-8
10.1515/zna-1972-0219 / Naturforsch. / Der Einfluß tiefer Temperaturen auf die Strahlenschädigung von organischen Kristallen durch 100 keV‐Elektronen. Z by Siegel G. (1972)
10.1016/0304-3991(84)90098-6
10.1007/978-3-642-72815-0_3 / Cryotechniques in Biological Electron Microscopy by Talmon Y. (1987)
10.1111/j.1365-2818.1986.tb02730.x
10.1111/j.1365-2818.1979.tb04689.x
10.1016/0304-3991(84)90096-2
10.1016/0304-3991(84)90094-9
10.1146/annurev.bb.15.060186.002035
10.1063/1.1697597
10.1002/jemt.1060090107

Dates

Type	When
Created	14 years ago (Aug. 2, 2011, 2:32 p.m.)
Deposited	1 year, 9 months ago (Oct. 22, 2023, 6:54 p.m.)
Indexed	1 year, 3 months ago (April 25, 2024, 10:04 p.m.)
Issued	34 years, 7 months ago (Jan. 1, 1991)
Published	34 years, 7 months ago (Jan. 1, 1991)
Published Online	14 years ago (Aug. 2, 2011)
Published Print	34 years, 7 months ago (Jan. 1, 1991)

Funders 0

None

BibTeX

@article{Lamvik_1991, title={Radiation damage in dry and frozen hydrated organic material}, volume={161}, ISSN={1365-2818}, url={http://dx.doi.org/10.1111/j.1365-2818.1991.tb03081.x}, DOI={10.1111/j.1365-2818.1991.tb03081.x}, number={1}, journal={Journal of Microscopy}, publisher={Wiley}, author={Lamvik, M. K.}, year={1991}, month=jan, pages={171–181} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        4,
        26
      ]
    ],
    "date-time": "2024-04-26T02:04:55Z",
    "timestamp": 1714097095932
  },
  "reference-count": 66,
  "publisher": "Wiley",
  "issue": "1",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2011,
            8,
            2
          ]
        ],
        "date-time": "2011-08-02T00:00:00Z",
        "timestamp": 1312243200000
      },
      "content-version": "vor",
      "delay-in-days": 7518,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [
      "onlinelibrary.wiley.com"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1991,
        1
      ]
    ]
  },
  "abstract": "<jats:title>SUMMARY</jats:title><jats:p>Electron\u2010induced radiation damage can cause errors in interpreting electron micrographs. Radiation damage is distinguished from contamination (polymerization of hydrocarbons) and etching (radiolysis in the presence of water), both of which can be controlled by a proper specimen environment in the microscope. While temperature has little effect on the primary interactions of fast electrons with matter, most secondary radiation\u2010damage processes are temperature dependent. Because damage mechanisms differ so greatly among materials, there is no simple factor by which specimen stability is improved as a function of temperature (some cases improve fivefold, others improve 100\u2010fold). While some specimens are stable to almost arbitrarily high doses, some tests reveal damage at 1 e/nm<jats:sup>2</jats:sup>. This paper surveys damage rates and temperature dependencies of various materials as a guide for future electron microscopic studies of organic specimens.</jats:p>",
  "DOI": "10.1111/j.1365-2818.1991.tb03081.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2011,
        8,
        2
      ]
    ],
    "date-time": "2011-08-02T18:32:54Z",
    "timestamp": 1312309974000
  },
  "page": "171-181",
  "update-policy": "http://dx.doi.org/10.1002/crossmark_policy",
  "source": "Crossref",
  "is-referenced-by-count": 38,
  "title": "Radiation damage in dry and frozen hydrated organic material",
  "prefix": "10.1111",
  "volume": "161",
  "author": [
    {
      "given": "M. K.",
      "family": "Lamvik",
      "sequence": "first",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2011,
        8,
        2
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_1",
      "first-page": "1115",
      "article-title": "The elementary composition of organic objects after electron irradiation",
      "volume": "14",
      "author": "Bahr G.F.",
      "year": "1965",
      "journal-title": "Lab. Invest."
    },
    {
      "key": "e_1_2_1_3_1",
      "first-page": "955",
      "article-title": "The determination of the dry mass in populations of isolated particles",
      "volume": "14",
      "author": "Bahr G.F.",
      "year": "1965",
      "journal-title": "Lab. Invest."
    },
    {
      "key": "e_1_2_1_4_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0022-2836(88)90288-4"
    },
    {
      "key": "e_1_2_1_5_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-3991(86)90095-1"
    },
    {
      "key": "e_1_2_1_6_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/178305a0"
    },
    {
      "key": "e_1_2_1_7_1",
      "first-page": "183",
      "volume-title": "Radiation Effects on Organic Materials",
      "author": "Bopp C.D.",
      "year": "1963"
    },
    {
      "key": "e_1_2_1_8_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF01333152"
    },
    {
      "key": "e_1_2_1_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2818.1986.tb02809.x"
    },
    {
      "key": "e_1_2_1_10_1",
      "first-page": "408",
      "volume-title": "Proc. 12th Int. Cong. on Electron Microscopy",
      "author": "Ciliax B.J.",
      "year": "1990"
    },
    {
      "key": "e_1_2_1_11_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/978-1-4684-6247-0"
    },
    {
      "key": "e_1_2_1_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2818.1972.tb01059.x"
    },
    {
      "key": "e_1_2_1_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-3991(83)90003-7"
    },
    {
      "key": "e_1_2_1_14_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-3991(82)90187-5"
    },
    {
      "key": "e_1_2_1_15_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2818.1982.tb04625.x"
    },
    {
      "key": "e_1_2_1_16_1",
      "first-page": "271",
      "article-title": "Electron irradiation effects in the EPMA quantitation of organic specimens",
      "volume": "2",
      "author": "Edie J.W.",
      "year": "1980",
      "journal-title": "Scanning Electron Microsc."
    },
    {
      "key": "e_1_2_1_17_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2818.1982.tb00360.x"
    },
    {
      "key": "e_1_2_1_18_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0304-3991(78)80037-0"
    },
    {
      "key": "e_1_2_1_19_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2818.1981.tb01267.x"
    },
    {
      "key": "e_1_2_1_20_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-3991(84)90221-3"
    },
    {
      "key": "e_1_2_1_21_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2818.1975.tb03896.x"
    },
    {
      "key": "e_1_2_1_22_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2818.1978.tb01160.x"
    },
    {
      "key": "e_1_2_1_23_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1080/14786437108217051"
    },
    {
      "key": "e_1_2_1_24_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2818.1986.tb02726.x"
    },
    {
      "key": "e_1_2_1_25_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2818.1974.tb03927.x"
    },
    {
      "key": "e_1_2_1_26_1",
      "first-page": "116",
      "article-title": "Die Objektverschmutzung im Elektronenmikroskop und das Problem der Strahlensch\u00e4digung durch Kohlenstoffabbau",
      "volume": "15",
      "author": "Heide H.\u2010G.",
      "year": "1963",
      "journal-title": "Z. Angew. Phys."
    },
    {
      "key": "e_1_2_1_27_1",
      "first-page": "1134",
      "article-title": "Contamination and irradiation effects and their dependence on the composition of residual gases in the electron microscope",
      "volume": "14",
      "author": "Heide H.\u2010G.",
      "year": "1965",
      "journal-title": "Lab. Invest."
    },
    {
      "key": "e_1_2_1_28_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-3991(82)90178-4"
    },
    {
      "key": "e_1_2_1_29_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-3991(84)90095-0"
    },
    {
      "key": "e_1_2_1_30_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-3991(85)90070-1"
    },
    {
      "key": "e_1_2_1_31_1",
      "first-page": "400",
      "volume-title": "Quantitative Electron Microscopy",
      "author": "Hobbs L.W.",
      "year": "1984"
    },
    {
      "key": "e_1_2_1_32_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-3991(80)90049-2"
    },
    {
      "key": "e_1_2_1_33_1",
      "first-page": "1",
      "volume-title": "Principles and Techniques of Electron Microscopy: Biological Applications",
      "author": "Isaacson M.S.",
      "year": "1977"
    },
    {
      "key": "e_1_2_1_34_1",
      "first-page": "61",
      "volume-title": "Proc. 9th Int. Cong. on Electron Microscopy",
      "author": "Isaacson M.",
      "year": "1978"
    },
    {
      "key": "e_1_2_1_35_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2818.1984.tb02544.x"
    },
    {
      "key": "e_1_2_1_36_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0022-2836(80)90382-4"
    },
    {
      "key": "e_1_2_1_37_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0022-2836(78)90108-0"
    },
    {
      "key": "e_1_2_1_38_1",
      "first-page": "816",
      "volume-title": "Proc. 47th Ann. Meet. EMSA.",
      "author": "Lamvik M.K.",
      "year": "1989"
    },
    {
      "key": "e_1_2_1_39_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jemt.1060080403"
    },
    {
      "key": "e_1_2_1_40_1",
      "first-page": "636",
      "volume-title": "Proc. 46th Ann. Meet. EMSA.",
      "author": "Lamvik M.K.",
      "year": "1988"
    },
    {
      "key": "e_1_2_1_41_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-3991(89)90017-X"
    },
    {
      "key": "e_1_2_1_42_1",
      "first-page": "271",
      "volume-title": "Scanning Microscopy",
      "author": "Lamvik M.K.",
      "year": "1989"
    },
    {
      "key": "e_1_2_1_43_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2818.1987.tb02867.x"
    },
    {
      "key": "e_1_2_1_44_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/301332a0"
    },
    {
      "key": "e_1_2_1_45_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-3991(84)90041-X"
    },
    {
      "key": "e_1_2_1_46_1",
      "first-page": "412",
      "volume-title": "Proc. 40th Ann. Meet. EMSA.",
      "author": "Leapman R.D.",
      "year": "1982"
    },
    {
      "key": "e_1_2_1_47_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0022-2836(83)90073-6"
    },
    {
      "key": "e_1_2_1_48_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2818.1983.tb04590.x"
    },
    {
      "key": "e_1_2_1_49_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.2307/3574071"
    },
    {
      "key": "e_1_2_1_50_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/133911b0"
    },
    {
      "key": "e_1_2_1_51_1",
      "volume-title": "Early History of the Electron Microscope",
      "author": "Marton L.",
      "year": "1968"
    },
    {
      "key": "e_1_2_1_52_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/ja00521a086"
    },
    {
      "key": "e_1_2_1_53_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0304-3991(85)80010-3"
    },
    {
      "key": "e_1_2_1_54_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/j100326a061"
    },
    {
      "key": "e_1_2_1_55_1",
      "first-page": "1082",
      "article-title": "Irradiation changes in organic and inorganic objects",
      "volume": "14",
      "author": "Reimer L.",
      "year": "1965",
      "journal-title": "Lab. Invest."
    },
    {
      "key": "e_1_2_1_56_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-3991(82)90039-0"
    },
    {
      "key": "e_1_2_1_57_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-3991(76)90049-8"
    },
    {
      "key": "e_1_2_1_58_1",
      "doi-asserted-by": "crossref",
      "first-page": "325",
      "DOI": "10.1515/zna-1972-0219",
      "article-title": "Der Einflu\u00df tiefer Temperaturen auf die Strahlensch\u00e4digung von organischen Kristallen durch 100 keV\u2010Elektronen. Z",
      "volume": "27",
      "author": "Siegel G.",
      "year": "1972",
      "journal-title": "Naturforsch."
    },
    {
      "key": "e_1_2_1_59_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-3991(84)90098-6"
    },
    {
      "key": "e_1_2_1_60_1",
      "doi-asserted-by": "crossref",
      "first-page": "64",
      "DOI": "10.1007/978-3-642-72815-0_3",
      "volume-title": "Cryotechniques in Biological Electron Microscopy",
      "author": "Talmon Y.",
      "year": "1987"
    },
    {
      "key": "e_1_2_1_61_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2818.1986.tb02730.x"
    },
    {
      "key": "e_1_2_1_62_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2818.1979.tb04689.x"
    },
    {
      "key": "e_1_2_1_63_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-3991(84)90096-2"
    },
    {
      "key": "e_1_2_1_64_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-3991(84)90094-9"
    },
    {
      "key": "e_1_2_1_65_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.bb.15.060186.002035"
    },
    {
      "key": "e_1_2_1_66_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1063/1.1697597"
    },
    {
      "key": "e_1_2_1_67_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jemt.1060090107"
    }
  ],
  "container-title": "Journal of Microscopy",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2818.1991.tb03081.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2818.1991.tb03081.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        10,
        22
      ]
    ],
    "date-time": "2023-10-22T22:54:49Z",
    "timestamp": 1698015289000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1111/j.1365-2818.1991.tb03081.x"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1991,
        1
      ]
    ]
  },
  "references-count": 66,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          1991,
          1
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1111/j.1365-2818.1991.tb03081.x"
  ],
  "URL": "http://dx.doi.org/10.1111/j.1365-2818.1991.tb03081.x",
  "relation": {},
  "ISSN": [
    "0022-2720",
    "1365-2818"
  ],
  "subject": [],
  "container-title-short": "Journal of Microscopy",
  "published": {
    "date-parts": [
      [
        1991,
        1
      ]
    ]
  },
  "assertion": [
    {
      "value": "2011-08-02",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}