Abstract
SUMMARYThe optical properties of a general scanning microscope are determined within the framework of Fourier imaging theory. For a simple model optical system, with Gaussian lens and detector apertures, the contrast transfer function can be expressed in terms of elementary functions. The theory predicts that spatial resolution and depth discrimination vary continuously with detector aperture and that defocus phase contrast is present in transmission images obtained with a symmetric objective, collector lens confocal microscope.
References
12
Referenced
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Dates
Type | When |
---|---|
Created | 14 years, 1 month ago (Aug. 2, 2011, 3:26 p.m.) |
Deposited | 1 year, 10 months ago (Oct. 20, 2023, 10:32 p.m.) |
Indexed | 1 year, 10 months ago (Oct. 23, 2023, 1:57 a.m.) |
Issued | 37 years, 8 months ago (Jan. 1, 1988) |
Published | 37 years, 8 months ago (Jan. 1, 1988) |
Published Online | 14 years, 1 month ago (Aug. 2, 2011) |
Published Print | 37 years, 8 months ago (Jan. 1, 1988) |
@article{Shuman_1988, title={Contrast in confocal scanning microscopy with a finite detector}, volume={149}, ISSN={1365-2818}, url={http://dx.doi.org/10.1111/j.1365-2818.1988.tb04562.x}, DOI={10.1111/j.1365-2818.1988.tb04562.x}, number={1}, journal={Journal of Microscopy}, publisher={Wiley}, author={Shuman, H.}, year={1988}, month=jan, pages={67–71} }