DOI: 10.1111/j.1365-2818.1986.tb02731.x. Cryoprotection in electron microscopy

10.1111/j.1365-2818.1986.tb02731.x

Crossref journal-article

Wiley

Journal of Microscopy (311)

Abstract

SUMMARYA set of experiments has been undertaken to resolve questions about the cryoprotection factor afforded by cooling specimens in the electron microscope. Two specimens, an n‐alkane (paraffin) and bacterial purple membrane, were prepared and distributed to several laboratories. Radiation damage studies were carried out at these laboratories independently, but following a standard protocol as closely as possible. While it was not possible to make all measurements at all of the laboratories, sufficient data have been obtained to establish a general reproducibility in the overall features of the cryoprotection effect from room temperature to near 4.2 K.

Bibliography

Cryoprotection in electron microscopy. (1986). Journal of Microscopy, 141(3), 385â391. Portico.

Authors 0

None

References 25 Referenced 75

10.1016/S0304-3991(81)80165-9
{'key': 'e_1_2_1_3_1', 'volume-title': 'Radiation Chemistry of Macromolecules', 'author': 'Dole M.', 'year': '1973'} / Radiation Chemistry of Macromolecules by Dole M. (1973)
10.1107/S0567739480001271
10.1016/0304-3991(83)90003-7
10.1111/j.1365-2818.1975.tb03896.x
10.1080/14786437108217051
10.1016/S0304-3991(79)90211-0
10.1073/pnas.75.9.4320
Heide H. G. Hermann K.‐H.&Jager J.(1982)Radiation damage of 1‐valine between 8 and 300 K.Proceedings Tenth International Congress on Electron Microscopy Hamburg Vol. 2 pp.457–456.
10.1038/257028a0
10.1016/0022-2836(84)90449-2
10.1016/0022-2836(80)90382-4
10.1016/0304-3991(82)90192-9
10.1016/S0304-3991(75)80007-6
10.1038/301332a0
10.1016/0022-2836(83)90073-6
10.1016/0304-3991(82)90039-0
10.1111/j.1365-2818.1975.tb04059.x
10.1515/zna-1972-0219 / Z. Naturforsch. / The influence of very low temperatures on the radiation damage of organic crystals irradiated by 100 keV electrons by Siegel G. (1972)
10.1107/S0365110X59000901
10.1016/0022-2836(75)90212-0
10.1016/0304-3991(84)90094-9
10.1016/0304-3991(82)90049-3
10.1016/0022-2836(70)90181-6
Zemlin F. Beckmann E. Reuber E. Dorset D.&Knapek E.(1984)Cryo‐electron microscopy of paraffin crystals.Proceedings Eighth European Congress on Electron Microscopy Congress Bureau MOTESZ Budapest pp.241–242.

Dates

Type	When
Created	14 years ago (Aug. 2, 2011, 9:20 a.m.)
Deposited	1 year, 10 months ago (Oct. 20, 2023, 9 a.m.)
Indexed	4 months ago (April 22, 2025, 1:44 p.m.)
Issued	39 years, 5 months ago (March 1, 1986)
Published	39 years, 5 months ago (March 1, 1986)
Published Online	14 years ago (Aug. 2, 2011)
Published Print	39 years, 5 months ago (March 1, 1986)

Funders 0

None

BibTeX

@article{1986, volume={141}, ISSN={1365-2818}, url={http://dx.doi.org/10.1111/j.1365-2818.1986.tb02731.x}, DOI={10.1111/j.1365-2818.1986.tb02731.x}, number={3}, journal={Journal of Microscopy}, publisher={Wiley}, year={1986}, month=mar, pages={385–391} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        4,
        22
      ]
    ],
    "date-time": "2025-04-22T17:44:26Z",
    "timestamp": 1745343866116
  },
  "reference-count": 25,
  "publisher": "Wiley",
  "issue": "3",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2011,
            8,
            2
          ]
        ],
        "date-time": "2011-08-02T00:00:00Z",
        "timestamp": 1312243200000
      },
      "content-version": "vor",
      "delay-in-days": 9285,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [
      "onlinelibrary.wiley.com"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1986,
        3
      ]
    ]
  },
  "abstract": "<jats:title>SUMMARY</jats:title><jats:p>A set of experiments has been undertaken to resolve questions about the cryoprotection factor afforded by cooling specimens in the electron microscope. Two specimens, an n\u2010alkane (paraffin) and bacterial purple membrane, were prepared and distributed to several laboratories. Radiation damage studies were carried out at these laboratories independently, but following a standard protocol as closely as possible. While it was not possible to make all measurements at all of the laboratories, sufficient data have been obtained to establish a general reproducibility in the overall features of the cryoprotection effect from room temperature to near 4.2 K.</jats:p>",
  "DOI": "10.1111/j.1365-2818.1986.tb02731.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2011,
        8,
        2
      ]
    ],
    "date-time": "2011-08-02T13:20:39Z",
    "timestamp": 1312291239000
  },
  "page": "385-391",
  "update-policy": "http://dx.doi.org/10.1002/crossmark_policy",
  "source": "Crossref",
  "is-referenced-by-count": 75,
  "title": "Cryoprotection in electron microscopy",
  "prefix": "10.1111",
  "volume": "141",
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2011,
        8,
        2
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0304-3991(81)80165-9"
    },
    {
      "key": "e_1_2_1_3_1",
      "volume-title": "Radiation Chemistry of Macromolecules",
      "author": "Dole M.",
      "year": "1973"
    },
    {
      "key": "e_1_2_1_4_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1107/S0567739480001271"
    },
    {
      "key": "e_1_2_1_5_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-3991(83)90003-7"
    },
    {
      "key": "e_1_2_1_6_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2818.1975.tb03896.x"
    },
    {
      "key": "e_1_2_1_7_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1080/14786437108217051"
    },
    {
      "key": "e_1_2_1_8_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0304-3991(79)90211-0"
    },
    {
      "key": "e_1_2_1_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.75.9.4320"
    },
    {
      "key": "e_1_2_1_10_1",
      "unstructured": "Heide H. G. Hermann K.\u2010H.&Jager J.(1982)Radiation damage of 1\u2010valine between 8 and 300 K.Proceedings Tenth International Congress on Electron Microscopy Hamburg Vol. 2 pp.457\u2013456."
    },
    {
      "key": "e_1_2_1_11_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/257028a0"
    },
    {
      "key": "e_1_2_1_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0022-2836(84)90449-2"
    },
    {
      "key": "e_1_2_1_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0022-2836(80)90382-4"
    },
    {
      "key": "e_1_2_1_14_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-3991(82)90192-9"
    },
    {
      "key": "e_1_2_1_15_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0304-3991(75)80007-6"
    },
    {
      "key": "e_1_2_1_16_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/301332a0"
    },
    {
      "key": "e_1_2_1_17_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0022-2836(83)90073-6"
    },
    {
      "key": "e_1_2_1_18_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-3991(82)90039-0"
    },
    {
      "key": "e_1_2_1_19_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2818.1975.tb04059.x"
    },
    {
      "key": "e_1_2_1_20_1",
      "doi-asserted-by": "crossref",
      "first-page": "325",
      "DOI": "10.1515/zna-1972-0219",
      "article-title": "The influence of very low temperatures on the radiation damage of organic crystals irradiated by 100 keV electrons",
      "volume": "27",
      "author": "Siegel G.",
      "year": "1972",
      "journal-title": "Z. Naturforsch."
    },
    {
      "key": "e_1_2_1_21_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1107/S0365110X59000901"
    },
    {
      "key": "e_1_2_1_22_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0022-2836(75)90212-0"
    },
    {
      "key": "e_1_2_1_23_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-3991(84)90094-9"
    },
    {
      "key": "e_1_2_1_24_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-3991(82)90049-3"
    },
    {
      "key": "e_1_2_1_25_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0022-2836(70)90181-6"
    },
    {
      "key": "e_1_2_1_26_1",
      "unstructured": "Zemlin F. Beckmann E. Reuber E. Dorset D.&Knapek E.(1984)Cryo\u2010electron microscopy of paraffin crystals.Proceedings Eighth European Congress on Electron Microscopy Congress Bureau MOTESZ Budapest pp.241\u2013242."
    }
  ],
  "container-title": "Journal of Microscopy",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2818.1986.tb02731.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2818.1986.tb02731.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        10,
        20
      ]
    ],
    "date-time": "2023-10-20T13:00:27Z",
    "timestamp": 1697806827000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1111/j.1365-2818.1986.tb02731.x"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1986,
        3
      ]
    ]
  },
  "references-count": 25,
  "journal-issue": {
    "issue": "3",
    "published-print": {
      "date-parts": [
        [
          1986,
          3
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1111/j.1365-2818.1986.tb02731.x"
  ],
  "URL": "http://dx.doi.org/10.1111/j.1365-2818.1986.tb02731.x",
  "relation": {},
  "ISSN": [
    "0022-2720",
    "1365-2818"
  ],
  "subject": [],
  "container-title-short": "Journal of Microscopy",
  "published": {
    "date-parts": [
      [
        1986,
        3
      ]
    ]
  },
  "assertion": [
    {
      "value": "2011-08-02",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}