DOI: 10.1111/j.1365-2818.1985.tb02663.x. An inexpensive device for freeze drying and plastic embedding tissues at low temperatures

An inexpensive device for freeze drying and plastic embedding tissues at low temperatures

10.1111/j.1365-2818.1985.tb02663.x

Crossref journal-article

Wiley

Journal of Microscopy (311)

Abstract

SUMMARYThe preparation of biological tissues for electron microscopy by rapid freezing retains the original localization of ions and molecules. A reproducible freezing regime was established by quenching tissues in liquid propane according to the method of Elder et al. (1981). Tissue was thereafter freeze dried in a custom built freeze drying device with a liquid nitrogen cooled stage to prevent ice recrystallization during drying. The device was also designed to allow the vacuum embedding of tissue in low temperature resin such as Lowicryl® and polymerization in situ. This paper describes the design of the device and an example of its use in the freeze drying of cartilage. The results show that minimal ice damage occurs to the chondrocytes and that intracellular organelles are clearly visible. The regime described may prove a useful and pragmatic alternative to cutting tissue in the frozen state. Translocation of elements is unlikely except perhaps in the case of very labile elements such as Na and K, but this remains to be fully elucidated.

Bibliography

Lyon, R., Appleton, J., Swindin, K. J., Abbot, J. J., & Chesters, J. (1985). An inexpensive device for freeze drying and plastic embedding tissues at low temperatures. Journal of Microscopy, 140(1), 81â91. Portico.

Authors 5

R. Lyon (first)
J. Appleton (additional)
K. J. Swindin (additional)
J. J. Abbot (additional)
J. Chesters (additional)

References 29 Referenced 2

10.1177/33.10.3900194
10.1177/27.2.448059
10.1007/BF00224942
10.1111/j.1365-2818.1969.tb00709.x
10.1111/j.1365-2818.1982.tb00362.x
{'key': 'e_1_2_1_7_1', 'first-page': '60', 'volume-title': 'Electron Microscopy', 'author': 'Coulter H. D.', 'year': '1978'} / Electron Microscopy by Coulter H. D. (1978)
10.1002/ar.1091870405
10.1111/j.1365-2818.1978.tb01172.x
10.1111/j.1365-2818.1982.tb00356.x
10.1111/j.1365-2818.1976.tb02402.x
10.1016/S0022-5320(60)80026-3
10.1177/33.4.3980981
Hohling H. J.(1972)Quantitative electron microscopy and electron probe microanalysis for studying hard tissue formation.Proceedings of the4th International Congress on Histochemistry and Cytochemistry p.89.
10.1007/BF00218713
10.1007/BF00623142
10.1007/BF01173116
{'key': 'e_1_2_1_18_1', 'first-page': '193', 'article-title': 'Quantitative analysis with the freeze dried plastic embedded tissue specimen', 'volume': '22', 'author': 'Ingram F. D.', 'year': '1975', 'journal-title': 'J. Microscopie'} / J. Microscopie / Quantitative analysis with the freeze dried plastic embedded tissue specimen by Ingram F. D. (1975)
{'key': 'e_1_2_1_19_1', 'first-page': '119', 'volume-title': 'Microprobe Analysis as Applied to Cells and Tissues', 'author': 'Ingram F. D.', 'year': '1974'} / Microprobe Analysis as Applied to Cells and Tissues by Ingram F. D. (1974)
10.1016/S0074-7696(08)61676-9
10.1021/j100677a022
10.1007/BF01291413
10.1016/0011-2240(80)90072-3
10.1016/S0022-5320(75)80083-9
10.1016/S0022-5320(69)90033-1
{'key': 'e_1_2_1_26_1', 'first-page': '122', 'volume-title': 'Recent Research in Freezing and Drying', 'author': 'Stephenson J. L.', 'year': '1960'} / Recent Research in Freezing and Drying by Stephenson J. L. (1960)
10.3109/10520295109113190
Terracio L.&Coulter H. D.(1975)Preparation of frozen dried tissue for transmission electron microscopy. A simple and dependable method. 33rd Ann. Proc. Elect. Microscop. Soc. Amer. pp.614–615. (10.1017/S0424820100116693)
{'key': 'e_1_2_1_29_1', 'first-page': '9', 'article-title': 'Berechnung von Gefriertrucknungzeiten für die elektronmikroskipische Preparation', 'volume': '40', 'author': 'Umrath V. W.', 'year': '1983', 'journal-title': 'Mikroskopie (Wien)'} / Mikroskopie (Wien) / Berechnung von Gefriertrucknungzeiten für die elektronmikroskipische Preparation by Umrath V. W. (1983)
10.1016/S0022-5320(77)80004-X

Dates

Type	When
Created	14 years, 1 month ago (Aug. 2, 2011, 7:54 a.m.)
Deposited	1 year, 10 months ago (Oct. 21, 2023, 5:36 p.m.)
Indexed	1 year, 10 months ago (Oct. 22, 2023, 4:42 a.m.)
Issued	39 years, 11 months ago (Oct. 1, 1985)
Published	39 years, 11 months ago (Oct. 1, 1985)
Published Online	14 years, 1 month ago (Aug. 2, 2011)
Published Print	39 years, 11 months ago (Oct. 1, 1985)

Funders 0

None

BibTeX

@article{Lyon_1985, title={An inexpensive device for freeze drying and plastic embedding tissues at low temperatures}, volume={140}, ISSN={1365-2818}, url={http://dx.doi.org/10.1111/j.1365-2818.1985.tb02663.x}, DOI={10.1111/j.1365-2818.1985.tb02663.x}, number={1}, journal={Journal of Microscopy}, publisher={Wiley}, author={Lyon, R. and Appleton, J. and Swindin, K. J. and Abbot, J. J. and Chesters, J.}, year={1985}, month=oct, pages={81–91} }

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