DOI: 10.1111/j.1365-2818.1981.tb01267.x. Comparative mass measurement of biological macromolecules by scanning transmission electron microscopy

Comparative mass measurement of biological macromolecules by scanning transmission electron microscopy

10.1111/j.1365-2818.1981.tb01267.x

Crossref journal-article

Wiley

Journal of Microscopy (311)

Abstract

SUMMARYA method is described for measuring the mass/length or mass of molecular assemblies by comparative electron scattering in the STEM. Standard particles whose mass is well established (e.g. TMV or fd bacteriophage) are deposited on the electron microscope grid together with the sample to be measured. Images containing at least one sample and standard and with a clean, contamination‐free background are chosen and stored on computer disc and then directly integrated. Use of a comparative technique does not require accurate determination of scattering parameters or instrumental geometry and requires only that the limits of linearity be established.The results of the mass/length measurements on phage pf 1, pili, muscle thick filaments and actin are in good agreement with existing molecular weight data and generally have a standard deviation of about 10%. The results for the total mass measurement of the multisubunit enzymes glutamate dehydrogenase and glutamine synthetase are also close to the literature values for their molecular weights. The results for the spherical, Semliki forest and tomato bushy stunt viruses are lower than expected, possibly reflecting some dissociation during preparation.

Bibliography

Freeman, R., & Leonard, K. R. (1981). Comparative mass measurement of biological macromolecules by scanning transmission electron microscopy. Journal of Microscopy, 122(3), 275â286. Portico.

Authors 2

R. Freeman (first)
K. R. Leonard (additional)

References 14 Referenced 60

10.1016/S0021-9258(19)86839-4 / J. biol. Chem. / Quaternary structure of pyruvate carboxylase from Pseudomonas citronellolis by Cohen N.D. (1979)
{'key': 'e_1_2_1_3_1', 'first-page': '605', 'volume-title': 'Single Stranded DNA Phages', 'author': 'Day L.A.', 'year': '1978'} / Single Stranded DNA Phages by Day L.A. (1978)
10.1016/S0304-3991(78)80037-0
10.1016/0022-2836(79)90423-6
{'key': 'e_1_2_1_6_1', 'first-page': '640', 'article-title': 'The temperature dependence of beam damage to biological samples in the scanning transmission electron microscope', 'volume': '2', 'author': 'Freeman R.', 'year': '1980', 'journal-title': 'Proceedings of the Seventh European Congress on Electron Microscopy'} / Proceedings of the Seventh European Congress on Electron Microscopy / The temperature dependence of beam damage to biological samples in the scanning transmission electron microscope by Freeman R. (1980)
10.1016/S0022-2836(61)80034-X
10.1038/271659a0
10.1159/000148837
10.1016/0022-2836(78)90108-0
10.1515/znb-1963-1106 / Z. Naturforsch. / A fibrous DNA phage (fd) and a spherical RNA phage (fr) specific for male strains of E. coli, Part II by Marvin D.A. (1963)
10.1007/BF00712061
10.1016/0014-5793(79)81179-5
10.1021/bi00846a017 / Biochemistry / Regulation of glutamine synthetase, xii. Electron microscopy of the enzyme from E. coli. by Valentine R.C. (1968)
10.1063/1.1777179

Dates

Type	When
Created	14 years ago (Aug. 2, 2011, 7:06 a.m.)
Deposited	1 year, 9 months ago (Nov. 10, 2023, 11:36 a.m.)
Indexed	1 year, 2 months ago (June 8, 2024, 12:34 a.m.)
Issued	44 years, 2 months ago (June 1, 1981)
Published	44 years, 2 months ago (June 1, 1981)
Published Online	14 years ago (Aug. 2, 2011)
Published Print	44 years, 2 months ago (June 1, 1981)

Funders 0

None

BibTeX

@article{Freeman_1981, title={Comparative mass measurement of biological macromolecules by scanning transmission electron microscopy}, volume={122}, ISSN={1365-2818}, url={http://dx.doi.org/10.1111/j.1365-2818.1981.tb01267.x}, DOI={10.1111/j.1365-2818.1981.tb01267.x}, number={3}, journal={Journal of Microscopy}, publisher={Wiley}, author={Freeman, R. and Leonard, K. R.}, year={1981}, month=jun, pages={275–286} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        6,
        8
      ]
    ],
    "date-time": "2024-06-08T04:34:50Z",
    "timestamp": 1717821290442
  },
  "reference-count": 14,
  "publisher": "Wiley",
  "issue": "3",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2011,
            8,
            2
          ]
        ],
        "date-time": "2011-08-02T00:00:00Z",
        "timestamp": 1312243200000
      },
      "content-version": "vor",
      "delay-in-days": 11019,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [
      "onlinelibrary.wiley.com"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1981,
        6
      ]
    ]
  },
  "abstract": "<jats:title>SUMMARY</jats:title><jats:p>A method is described for measuring the mass/length or mass of molecular assemblies by comparative electron scattering in the STEM. Standard particles whose mass is well established (e.g. TMV or fd bacteriophage) are deposited on the electron microscope grid together with the sample to be measured. Images containing at least one sample and standard and with a clean, contamination\u2010free background are chosen and stored on computer disc and then directly integrated. Use of a comparative technique does not require accurate determination of scattering parameters or instrumental geometry and requires only that the limits of linearity be established.</jats:p><jats:p>The results of the mass/length measurements on phage pf 1, pili, muscle thick filaments and actin are in good agreement with existing molecular weight data and generally have a standard deviation of about 10%. The results for the total mass measurement of the multisubunit enzymes glutamate dehydrogenase and glutamine synthetase are also close to the literature values for their molecular weights. The results for the spherical, Semliki forest and tomato bushy stunt viruses are lower than expected, possibly reflecting some dissociation during preparation.</jats:p>",
  "DOI": "10.1111/j.1365-2818.1981.tb01267.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2011,
        8,
        2
      ]
    ],
    "date-time": "2011-08-02T11:06:39Z",
    "timestamp": 1312283199000
  },
  "page": "275-286",
  "update-policy": "http://dx.doi.org/10.1002/crossmark_policy",
  "source": "Crossref",
  "is-referenced-by-count": 60,
  "title": "Comparative mass measurement of biological macromolecules by scanning transmission electron microscopy",
  "prefix": "10.1111",
  "volume": "122",
  "author": [
    {
      "given": "R.",
      "family": "Freeman",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "K. R.",
      "family": "Leonard",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2011,
        8,
        2
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_1",
      "doi-asserted-by": "crossref",
      "first-page": "9262",
      "DOI": "10.1016/S0021-9258(19)86839-4",
      "article-title": "Quaternary structure of pyruvate carboxylase from Pseudomonas citronellolis",
      "volume": "254",
      "author": "Cohen N.D.",
      "year": "1979",
      "journal-title": "J. biol. Chem."
    },
    {
      "key": "e_1_2_1_3_1",
      "first-page": "605",
      "volume-title": "Single Stranded DNA Phages",
      "author": "Day L.A.",
      "year": "1978"
    },
    {
      "key": "e_1_2_1_4_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0304-3991(78)80037-0"
    },
    {
      "key": "e_1_2_1_5_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0022-2836(79)90423-6"
    },
    {
      "key": "e_1_2_1_6_1",
      "first-page": "640",
      "article-title": "The temperature dependence of beam damage to biological samples in the scanning transmission electron microscope",
      "volume": "2",
      "author": "Freeman R.",
      "year": "1980",
      "journal-title": "Proceedings of the Seventh European Congress on Electron Microscopy"
    },
    {
      "key": "e_1_2_1_7_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0022-2836(61)80034-X"
    },
    {
      "key": "e_1_2_1_8_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/271659a0"
    },
    {
      "key": "e_1_2_1_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1159/000148837"
    },
    {
      "key": "e_1_2_1_10_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0022-2836(78)90108-0"
    },
    {
      "key": "e_1_2_1_11_1",
      "doi-asserted-by": "crossref",
      "first-page": "884",
      "DOI": "10.1515/znb-1963-1106",
      "article-title": "A fibrous DNA phage (fd) and a spherical RNA phage (fr) specific for male strains of E. coli, Part II",
      "volume": "18",
      "author": "Marvin D.A.",
      "year": "1963",
      "journal-title": "Z. Naturforsch."
    },
    {
      "key": "e_1_2_1_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00712061"
    },
    {
      "key": "e_1_2_1_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(79)81179-5"
    },
    {
      "key": "e_1_2_1_14_1",
      "doi-asserted-by": "crossref",
      "first-page": "2143",
      "DOI": "10.1021/bi00846a017",
      "article-title": "Regulation of glutamine synthetase, xii. Electron microscopy of the enzyme from E. coli.",
      "volume": "7",
      "author": "Valentine R.C.",
      "year": "1968",
      "journal-title": "Biochemistry"
    },
    {
      "key": "e_1_2_1_15_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1063/1.1777179"
    }
  ],
  "container-title": "Journal of Microscopy",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2818.1981.tb01267.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2818.1981.tb01267.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        11,
        10
      ]
    ],
    "date-time": "2023-11-10T16:36:17Z",
    "timestamp": 1699634177000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1111/j.1365-2818.1981.tb01267.x"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1981,
        6
      ]
    ]
  },
  "references-count": 14,
  "journal-issue": {
    "issue": "3",
    "published-print": {
      "date-parts": [
        [
          1981,
          6
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1111/j.1365-2818.1981.tb01267.x"
  ],
  "URL": "http://dx.doi.org/10.1111/j.1365-2818.1981.tb01267.x",
  "relation": {},
  "ISSN": [
    "0022-2720",
    "1365-2818"
  ],
  "subject": [],
  "container-title-short": "Journal of Microscopy",
  "published": {
    "date-parts": [
      [
        1981,
        6
      ]
    ]
  },
  "assertion": [
    {
      "value": "2011-08-02",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}