Biological X‐Ray Microanalysis
10.1111/j.1365-2818.1979.tb00236.x
Crossref journal-article
Wiley
Journal of Microscopy (311)
Abstract

SUMMARYBy means of X‐ray microanalysis it is now practical to detect approximately 10−19 g of an element in a static‐probe analysis within an ultrathin section, with analytical spatial resolution in the range 20–30 nm. The main difficulties for biological microanalysis are connected not with sensitivity but with specimen preparation and beam damage.Careful cryopreparation, beginning with the quench‐freezing of a small block of tissue, is essential even for determining the storage sites, or sites of binding in vivo, of physiologically active elements.In frozen‐dried or frozen‐hydrated sections of quench‐frozen tissue, it is now possible to measure local mass fractions of diffusible as well as of bound elements.

Bibliography

Hall, T. A. (1979). Biological X‐Ray Microanalysis. Journal of Microscopy, 117(1), 145–163. Portico.

Authors 1
  1. T. A. Hall (first)
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Dates
Type When
Created 14 years ago (Aug. 2, 2011, 8:27 a.m.)
Deposited 1 year, 9 months ago (Nov. 10, 2023, 4:23 p.m.)
Indexed 3 months ago (May 14, 2025, 9:29 a.m.)
Issued 45 years, 11 months ago (Sept. 1, 1979)
Published 45 years, 11 months ago (Sept. 1, 1979)
Published Online 14 years ago (Aug. 2, 2011)
Published Print 45 years, 11 months ago (Sept. 1, 1979)
Funders 0

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@article{Hall_1979, title={Biological X‐Ray Microanalysis}, volume={117}, ISSN={1365-2818}, url={http://dx.doi.org/10.1111/j.1365-2818.1979.tb00236.x}, DOI={10.1111/j.1365-2818.1979.tb00236.x}, number={1}, journal={Journal of Microscopy}, publisher={Wiley}, author={Hall, T. A.}, year={1979}, month=sep, pages={145–163} }