DOI: 10.1111/j.1365-2818.1978.tb02454.x. Radiation damage in the high resolution electron microscopy of biological materials: A review*

Radiation damage in the high resolution electron microscopy of biological materials: A review*

10.1111/j.1365-2818.1978.tb02454.x

Crossref journal-article

Wiley

Journal of Microscopy (311)

Abstract

SUMMARYRadiation damage to a biological specimen arises from a variety of interactions between the illuminating electrons and the atoms in it. The relative probabilities of these events, and the amount of energy transferred, can be calculated from basic physical theory. The microscopic damage caused in a particular specimen in given operating conditions is more difficult to predict, but it can be measured by a number of macroscopic indicators, the chief of which are loss of mass and changes in the energy loss spectrum (or electron diffraction pattern, if any). For most biological material the observed rate of damage is such as to set a limit to the intensity of illumination, the maximum magnification and the minimum size of detail that can be made visible. Several techniques have been devised and tested for reducing the radiation sensitivity of a specimen, of which cooling to a very low temperature and encasing it in an inert medium are the most effective. If the various protective measures act cooperatively, they could increase the effective resolution of sensitive material by an order of magnitude, making possible electron microscopy of the atomic structure of, for instance, the nucleic acid bases and other macromolecules. The prospects for observing living cells at a resolution better than that of the best optical microscopes would remain very small.

Bibliography

Cosslett, V. E. (1978). Radiation damage in the high resolution electron microscopy of biological materials: A review*. Journal of Microscopy, 113(2), 113â129. Portico.

Authors 1

V. E. Cosslett (first)

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Dates

Type	When
Created	14 years ago (Aug. 2, 2011, 9:44 a.m.)
Deposited	1 year, 4 months ago (April 9, 2024, 9:37 a.m.)
Indexed	2 weeks, 6 days ago (Aug. 2, 2025, 1:01 a.m.)
Issued	47 years, 1 month ago (July 1, 1978)
Published	47 years, 1 month ago (July 1, 1978)
Published Online	14 years ago (Aug. 2, 2011)
Published Print	47 years, 1 month ago (July 1, 1978)

Funders 0

None

BibTeX

@article{Cosslett_1978, title={Radiation damage in the high resolution electron microscopy of biological materials: A review*}, volume={113}, ISSN={1365-2818}, url={http://dx.doi.org/10.1111/j.1365-2818.1978.tb02454.x}, DOI={10.1111/j.1365-2818.1978.tb02454.x}, number={2}, journal={Journal of Microscopy}, publisher={Wiley}, author={Cosslett, V. E.}, year={1978}, month=jul, pages={113–129} }

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