DOI: 10.1111/j.1365-2818.1978.tb01160.x. Radiation damage relative to transmission electron microscopy of biological specimens at low temperature: a review

Radiation damage relative to transmission electron microscopy of biological specimens at low temperature: a review

10.1111/j.1365-2818.1978.tb01160.x

Crossref journal-article

Wiley

Journal of Microscopy (311)

Abstract

SUMMARYWhen biological specimens are irradiated by the electron beam in the electron microscope, the specimen structure is damaged as a result of molecular excitation, ionization, and subsequent chemical reactions. The radiation damage that occurs in the normal process of electron microscopy is known to present severe limitations for imaging high resolution detail in biological specimens. The question of radiation damage at low temperatures has therefore been investigated with the view in mind of reducing somewhat the rate at which damage occurs. The radiation damage protection found for small molecule (anhydrous) organic compounds is generally rather limited or even non‐existent. However, large molecule, hydrated materials show as much as a 10‐fold reduction at low temperature in the rate at which radiation damage occurs, relative to the damage rate at room temperature. In the case of hydrated specimens, therefore, low temperature electron microscopy offers an important advantage as part of the overall effort required in obtaining high resolution images of complex biological structures.

Bibliography

Glaeser, R. M., & Taylor, K. A. (1978). Radiation damage relative to transmission electron microscopy of biological specimens at low temperature: a review. Journal of Microscopy, 112(1), 127â138. Portico.

Authors 2

Robert M. Glaeser (first)
Kenneth A. Taylor (additional)

References 27 Referenced 208

{'key': 'e_1_2_1_2_1', 'first-page': '279', 'volume-title': 'Physical Aspects of Electron Microscopy and Microbeam Analysis', 'author': 'Box H. C.', 'year': '1975'} / Physical Aspects of Electron Microscopy and Microbeam Analysis by Box H. C. (1975)
{'key': 'e_1_2_1_3_1', 'volume-title': 'The Radiation Chemistry of Water (Physical Chemistry, a series of monographs)', 'author': 'Draganic I. G.', 'year': '1971'} / The Radiation Chemistry of Water (Physical Chemistry, a series of monographs) by Draganic I. G. (1971)
10.1016/S0022-5320(75)80118-3
10.1016/S0022-5320(71)80118-1
{'key': 'e_1_2_1_6_1', 'first-page': '205', 'volume-title': 'Physical Aspects of Electron Microscopy and Microbeam Analysis', 'author': 'Glaeser R. M.', 'year': '1975'} / Physical Aspects of Electron Microscopy and Microbeam Analysis by Glaeser R. M. (1975)
{'key': 'e_1_2_1_7_1', 'first-page': '133', 'article-title': 'Low temperature electron microscopy: radiation damage in crystalline biological materials', 'volume': '12', 'author': 'Glaeser R. M.', 'year': '1971', 'journal-title': 'J. Microscopie'} / J. Microscopie / Low temperature electron microscopy: radiation damage in crystalline biological materials by Glaeser R. M. (1971)
10.1111/j.1365-2818.1975.tb03896.x
{'key': 'e_1_2_1_9_1', 'first-page': '69', 'volume-title': 'Frozen hydrated biological specimens for electron microscopy and structure analysis', 'author': 'Glaeser R. M.', 'year': '1976'} / Frozen hydrated biological specimens for electron microscopy and structure analysis by Glaeser R. M. (1976)
10.1107/S0567740870003485
10.1016/0022-2836(76)90271-0
{'key': 'e_1_2_1_12_1', 'first-page': '183', 'volume-title': 'Comparative Effects of Radiation', 'author': 'Hochanadel C. J.', 'year': '1960'} / Comparative Effects of Radiation by Hochanadel C. J. (1960)
{'key': 'e_1_2_1_13_1', 'first-page': '247', 'volume-title': 'Physical Aspects of Electron Microscopy and Microbeam Analysis', 'author': 'Isaacson M. S.', 'year': '1975'} / Physical Aspects of Electron Microscopy and Microbeam Analysis by Isaacson M. S. (1975)
{'key': 'e_1_2_1_14_1', 'first-page': '1', 'volume-title': 'Principles and Techniques of Electron Microscopy', 'author': 'Isaacson M. S.', 'year': '1977'} / Principles and Techniques of Electron Microscopy by Isaacson M. S. (1977)
10.2307/3573678
{'key': 'e_1_2_1_16_1', 'first-page': '1097', 'article-title': 'Irradiation changes in organic polymers at various accelerating voltages', 'volume': '14', 'author': 'Kobayashi K.', 'year': '1975', 'journal-title': 'Lab. Invest.'} / Lab. Invest. / Irradiation changes in organic polymers at various accelerating voltages by Kobayashi K. (1975)
{'key': 'e_1_2_1_17_1', 'volume-title': 'Early History of the Electron Microscope', 'author': 'Marton L.', 'year': '1968'} / Early History of the Electron Microscope by Marton L. (1968)
10.1126/science.177.4045.268
{'key': 'e_1_2_1_19_1', 'first-page': '259', 'volume-title': 'Physical Aspects of Electron Microscopy and Microbeam Analysis', 'author': 'Parsons D. F.', 'year': '1975'} / Physical Aspects of Electron Microscopy and Microbeam Analysis by Parsons D. F. (1975)
10.1016/0022-2836(75)90167-9
{'key': 'e_1_2_1_21_1', 'first-page': '69', 'volume-title': 'Formation and Trapping of Free Radicals', 'author': 'Pimentel G. C.', 'year': '1960'} / Formation and Trapping of Free Radicals by Pimentel G. C. (1960)
10.1016/S0304-3991(75)80019-2
{'key': 'e_1_2_1_23_1', 'first-page': '231', 'volume-title': 'Physical Aspects of Electron Microscopy and Microbeam Analysis', 'author': 'Reimer L.', 'year': '1975'} / Physical Aspects of Electron Microscopy and Microbeam Analysis by Reimer L. (1975)
10.1515/zna-1972-0219 / Z. Naturforsch / Der Einfluss tiefer Temperaturen auf die strahlen Schädigung von organischen Kristallen durch 100 keV‐elektronen by Siegel G. (1972)
10.1016/S0022-5320(76)80099-8
10.1016/0022-2836(75)90212-0
10.1063/1.1660782
10.1126/science.180.4086.553

Dates

Type	When
Created	14 years ago (Aug. 2, 2011, 1:04 p.m.)
Deposited	1 year, 9 months ago (Nov. 10, 2023, 8:23 p.m.)
Indexed	1 month, 2 weeks ago (July 3, 2025, 6:44 a.m.)
Issued	47 years, 7 months ago (Jan. 1, 1978)
Published	47 years, 7 months ago (Jan. 1, 1978)
Published Online	14 years ago (Aug. 2, 2011)
Published Print	47 years, 7 months ago (Jan. 1, 1978)

Funders 0

None

BibTeX

@article{Glaeser_1978, title={Radiation damage relative to transmission electron microscopy of biological specimens at low temperature: a review}, volume={112}, ISSN={1365-2818}, url={http://dx.doi.org/10.1111/j.1365-2818.1978.tb01160.x}, DOI={10.1111/j.1365-2818.1978.tb01160.x}, number={1}, journal={Journal of Microscopy}, publisher={Wiley}, author={Glaeser, Robert M. and Taylor, Kenneth A.}, year={1978}, month=jan, pages={127–138} }

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