DOI: 10.1111/j.1365-2818.1978.tb01158.x. The use of low temperature X‐ray diffraction to evaluate freezing methods used in freeze‐fracture electron microscopy

The use of low temperature X‐ray diffraction to evaluate freezing methods used in freeze‐fracture electron microscopy

10.1111/j.1365-2818.1978.tb01158.x

Crossref journal-article

Wiley

Journal of Microscopy (311)

Abstract

SUMMARYTwo methods of freezing samples for freeze‐fracture electron microscopy have been compared using X‐ray diffraction and freeze‐fracture results from lipid‐water model systems. Perturbations of the molecular organization of hydrocarbon chains and the extent of ice crystal formation have been evaluated for lamellar phases of egg lecithin containing 16% water and egg lecithin‐phosphatidylinositol containing 55% water, both freeze‐quenched in liquid Freon‐22 near its melting temperature (113 K). Very thin samples sandwiched between copper sheets separated by an electron microscope grid show much less freezing induced structural rearrangement than small samples contained on conventional Balzers‐type gold planchettes. These results show that the rate of freezing in the very thin preparations is greater than in the conventional ones, which is probably due in part to the improved dissipation of heat from a poorly conductive sample through highly conductive copper sheets.

Bibliography

GulikâKrzywicki, T., & Costello, M. J. (1978). The use of low temperature Xâray diffraction to evaluate freezing methods used in freezeâfracture electron microscopy. Journal of Microscopy, 112(1), 103â113. Portico.

Authors 2

T. Gulik‐Krzywicki (first)
M. J. Costello (additional)

References 9 Referenced 94

10.1111/j.1365-2818.1978.tb01151.x
10.1016/0005-2736(76)90315-1
10.1016/0005-2736(70)90060-X
Gulik‐Krzywicki T. Costello M. J. Gadet A.&Fourme R.(1976)The use of low temperature X‐ray diffraction to evaluate freezing methods used in freeze‐fracture electron microscopy Proc. Sixth European Congress on Electron Microscopy Jerusalem Vol. II p.625.
{'key': 'e_1_2_1_6_1', 'volume-title': 'Biological Membranes', 'author': 'Luzzati V.', 'year': '1968'} / Biological Membranes by Luzzati V. (1968)
10.1016/0022-2836(74)90363-5
10.1016/0022-2836(67)90192-1
10.1016/0022-2836(73)90303-3
{'key': 'e_1_2_1_10_1', 'volume-title': 'Handbook of Chemistry and Physics', 'author': 'Weast R. C.', 'year': '1969'} / Handbook of Chemistry and Physics by Weast R. C. (1969)

Dates

Type	When
Created	14 years ago (Aug. 2, 2011, 1:04 p.m.)
Deposited	1 year, 9 months ago (Nov. 10, 2023, 8:22 p.m.)
Indexed	11 months, 3 weeks ago (Sept. 4, 2024, 4:55 p.m.)
Issued	47 years, 7 months ago (Jan. 1, 1978)
Published	47 years, 7 months ago (Jan. 1, 1978)
Published Online	14 years ago (Aug. 2, 2011)
Published Print	47 years, 7 months ago (Jan. 1, 1978)

Funders 0

None

BibTeX

@article{Gulik_Krzywicki_1978, title={The use of low temperature X‐ray diffraction to evaluate freezing methods used in freeze‐fracture electron microscopy}, volume={112}, ISSN={1365-2818}, url={http://dx.doi.org/10.1111/j.1365-2818.1978.tb01158.x}, DOI={10.1111/j.1365-2818.1978.tb01158.x}, number={1}, journal={Journal of Microscopy}, publisher={Wiley}, author={Gulik‐Krzywicki, T. and Costello, M. J.}, year={1978}, month=jan, pages={103–113} }

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