DOI: 10.1111/j.1365-2818.1977.tb01147.x. Estimation of the average section thickness in ribbons of ultra‐thin sections

Estimation of the average section thickness in ribbons of ultra‐thin sections

10.1111/j.1365-2818.1977.tb01147.x

Crossref journal-article

Wiley

Journal of Microscopy (311)

Abstract

SUMMARYA simple procedure for estimating the depth to which a ribbon of ultra‐thin sections cuts into a block is described. The average section increment is obtained by dividing the depth by the number of sections in the ribbon. Comparison with measurements of individual sections made using interference microscopy suggests: (a) that loss of resin brought about by the cutting process is insignificant, i.e. that the average section increment equals the average section thickness, and (b) that there can be considerable variation in section thickness along a ribbon. Provided that such variation is taken into account, the method based on measurements of the block face is simple, avoids time‐consuming alternative procedures, and is appropriate when seeking to determine the depth dimension to be used in making three‐dimensional reconstructions from long sequences of sections.

Bibliography

Gunning, B. E. S., & Hardham, A. R. (1977). Estimation of the average section thickness in ribbons of ultraâthin sections. Journal of Microscopy, 109(3), 337â340. Portico.

Authors 2

B. E. S. Gunning (first)
A. R. Hardham (additional)

References 7 Referenced 9

10.1111/j.1365-2818.1975.tb03991.x
10.1083/jcb.49.3.947
{'key': 'e_1_2_1_4_1', 'volume-title': 'Principles and Techniques of Electron Microscopy: Biological Applications', 'author': 'Hayat M. A.', 'year': '1970'} / Principles and Techniques of Electron Microscopy: Biological Applications by Hayat M. A. (1970)
{'key': 'e_1_2_1_5_1', 'volume-title': 'Practical Methods in Electron Microscopy', 'author': 'Reid N.', 'year': '1974'} / Practical Methods in Electron Microscopy by Reid N. (1974)
{'key': 'e_1_2_1_6_1', 'volume-title': 'Electron Microscopy of Cells and Tissues, Vol. 1; Instrumentation and Techniques', 'author': 'Sjostrand F. S.', 'year': '1967'} / Electron Microscopy of Cells and Tissues, Vol. 1; Instrumentation and Techniques by Sjostrand F. S. (1967)
Small J. V.(1968)Measurement of section thickness.Proc4th Europ. Conf. on Electron Microscopy Vol. 1 p.609.
10.1111/j.1365-2818.1966.tb02193.x

Dates

Type	When
Created	14 years ago (Aug. 2, 2011, 1:25 p.m.)
Deposited	1 year, 9 months ago (Nov. 10, 2023, 2:58 p.m.)
Indexed	11 months, 1 week ago (Sept. 8, 2024, 7:53 p.m.)
Issued	48 years, 4 months ago (April 1, 1977)
Published	48 years, 4 months ago (April 1, 1977)
Published Online	14 years ago (Aug. 2, 2011)
Published Print	48 years, 4 months ago (April 1, 1977)

Funders 0

None

BibTeX

@article{Gunning_1977, title={Estimation of the average section thickness in ribbons of ultra‐thin sections}, volume={109}, ISSN={1365-2818}, url={http://dx.doi.org/10.1111/j.1365-2818.1977.tb01147.x}, DOI={10.1111/j.1365-2818.1977.tb01147.x}, number={3}, journal={Journal of Microscopy}, publisher={Wiley}, author={Gunning, B. E. S. and Hardham, A. R.}, year={1977}, month=apr, pages={337–340} }

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