DOI: 10.1111/j.1365-2818.1976.tb02406.x. A copper block method for freezing non‐cryoprotected tissue to produce ice‐crystal‐free regions for electron microscopy

A copper block method for freezing non‐cryoprotected tissue to produce ice‐crystal‐free regions for electron microscopy

10.1111/j.1365-2818.1976.tb02406.x

Crossref journal-article

Wiley

Journal of Microscopy (311)

Abstract

SUMMARYTwo methods are described for fracturing unfixed, uncryoprotected mouse liver that had been frozen by contact with a copper block at liquid nitrogen temperature. The best method involved precise fracturing of a few microns of surface tissue with a cooled glass knife, using the Cryokit attachment of the LKB III ultramicrotome. The resulting replicas were free from electron microscopically‐visible ice crystals. A noticeable feature of replicas from unfixed, uncryoprotected tissue was the extent of plastic deformation of certain cellular structures. Cytoplasmic macromolecules, and to a lesser extent intramembranous particles, often appeared to be ‘stretched’ to form fibrils.

Bibliography

Dempsey, G. P., & Bullivant, S. (1976). A copper block method for freezing nonâcryoprotected tissue to produce iceâcrystalâfree regions for electron microscopy. Journal of Microscopy, 106(3), 261â271. Portico.

Authors 2

G. P. Dempsey (first)
S. Bullivant (additional)

References 14 Referenced 43

10.1073/pnas.68.9.2149
10.1016/0047-7206(69)90006-5
10.1007/978-3-642-65492-3_3
10.1083/jcb.29.3.435
10.1083/jcb.55.2.520
10.1007/BF00455276
10.1111/j.1365-2818.1976.tb02405.x
{'key': 'e_1_2_1_9_1', 'first-page': '529', 'article-title': 'The study of frozen specimens, ice crystals and ice crystal growth by electron microscopy', 'volume': '13', 'author': 'Meryman H.T.', 'year': '1955', 'journal-title': 'Res. Rep. nav. med. Res. Inst. Bethesda, Maryland'} / Res. Rep. nav. med. Res. Inst. Bethesda, Maryland / The study of frozen specimens, ice crystals and ice crystal growth by electron microscopy by Meryman H.T. (1955)
10.1007/BF01251613
10.1083/jcb.53.1.116
10.1111/j.1365-2818.1974.tb03880.x
10.1111/j.1365-2818.1974.tb03881.x
10.1016/S0074-7696(08)60940-7
10.1002/ar.1091490307

Dates

Type	When
Created	14 years ago (Aug. 2, 2011, 1:41 p.m.)
Deposited	1 year, 9 months ago (Nov. 10, 2023, 6:01 p.m.)
Indexed	1 year, 9 months ago (Nov. 10, 2023, 8:39 p.m.)
Issued	49 years, 5 months ago (April 1, 1976)
Published	49 years, 5 months ago (April 1, 1976)
Published Online	14 years ago (Aug. 2, 2011)
Published Print	49 years, 5 months ago (April 1, 1976)

Funders 0

None

BibTeX

@article{Dempsey_1976, title={A copper block method for freezing non‐cryoprotected tissue to produce ice‐crystal‐free regions for electron microscopy: II. Evaluation using freeze fracturing with a cryo‐ultramicrotome}, volume={106}, ISSN={1365-2818}, url={http://dx.doi.org/10.1111/j.1365-2818.1976.tb02406.x}, DOI={10.1111/j.1365-2818.1976.tb02406.x}, number={3}, journal={Journal of Microscopy}, publisher={Wiley}, author={Dempsey, G. P. and Bullivant, S.}, year={1976}, month=apr, pages={261–271} }

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