DOI: 10.1111/j.1365-2818.1975.tb04034.x. A new method for comparative light and electron microscopic studies of individual cells, selected in the living state

A new method for comparative light and electron microscopic studies of individual cells, selected in the living state

10.1111/j.1365-2818.1975.tb04034.x

Crossref journal-article

Wiley

Journal of Microscopy (311)

Abstract

SUMMARYA method is described which permits comparative light and electronmicroscopic studies of cell cultures, cell spreads or single selected cells which have been kept in the Plastic Film Dish (PFD). The PFD is a versatile large surface tissue culture chamber which, for electron microscopy, is mounted with a transparent FEP‐Teflon film bottom. Cells are observed, selected and marked on the PFD‐bottom with a high power inverted light microscope. The cells are fixed and dehydrated with a semi‐automatic device while they are still in situ in the PFD. During the preparation steps for electron microscopy the topographical relationship between individual cells and between cells and cell support is accurately retained. After embedding and polymerization the Teflon film is easily peeled off the polymerized Epon, leaving a replica of the mark around the selected cell. This permits relocation of the selected cells for ultrathin sectioning in a plane plan‐parallel to the original cell support. To enable orientated sectioning of selected cells in a plane perpendicular to the cell support, cells are tagged with Letraset‐letters after original embedding and polymerization. Subsequently the re‐embedded polymerized specimens are orientated in the microtome in a position which permits controlled thin sectioning of the tagged cells in the previously selected plane.

Bibliography

van Ewijk, W., & HÃ¶sli, P. (1975). A new method for comparative light and electron microscopic studies of individual cells, selected in the living state. Journal of Microscopy, 105(1), 19â31. Portico.

Authors 2

W. van Ewijk (first)
P. Hösli (additional)

References 32 Referenced 12

10.1083/jcb.7.1.191
{'key': 'e_1_2_1_3_1', 'first-page': '68', 'article-title': 'A new technique for comparative phase‐contrast and electron microscope studies of cells grown in tissue culture, with an evaluation of the technique by means of time‐lapse cinemicrographs', 'volume': '95', 'author': 'Borysko E.', 'year': '1954', 'journal-title': 'Bull. Johns Hopkins Hosp.'} / Bull. Johns Hopkins Hosp. / A new technique for comparative phase‐contrast and electron microscope studies of cells grown in tissue culture, with an evaluation of the technique by means of time‐lapse cinemicrographs by Borysko E. (1954)
10.1016/S0014-4827(71)80076-9
10.1083/jcb.35.1.279
{'key': 'e_1_2_1_6_1', 'first-page': '295', 'article-title': 'A simple technique for comparative light and electron microscopy of designated living cultured cells', 'volume': '25', 'author': 'Buckley I.K.', 'year': '1971', 'journal-title': 'Lab. Invest.'} / Lab. Invest. / A simple technique for comparative light and electron microscopy of designated living cultured cells by Buckley I.K. (1971)
10.1016/S0022-5320(71)80130-2
10.3109/10520297009085350
10.1111/j.1365-2818.1964.tb00561.x
10.3109/10520296809115071
10.3109/10520296509116442
10.3109/10520296609116304 / Stain Technol. / Polyester sheeting (Melinex O), a tissue‐culture support easily separable from epoxy resins after flat‐face embedding by Firket H. (1966)
10.1016/0014-4827(69)90528-X
{'key': 'e_1_2_1_14_1', 'first-page': '335', 'article-title': 'In situ embedding of cultured cells or tissue, grown on glass, in epoxy resins for electron microscopy', 'volume': '38', 'author': 'Heyner S.', 'year': '1963', 'journal-title': 'Stain Technol.'} / Stain Technol. / In situ embedding of cultured cells or tissue, grown on glass, in epoxy resins for electron microscopy by Heyner S. (1963)
10.1083/jcb.4.1.115
Hösli P.(1972)Tissue cultivation on plastic films. Tecnomara A.G. Zürich CH‐8059.
{'key': 'e_1_2_1_17_1', 'first-page': '226', 'volume-title': 'Birth Defects. Proceedings of the Fourth International Conference, Vienna, Austria, 2–8 September 1973', 'author': 'Hösli P.', 'year': '1974'} / Birth Defects. Proceedings of the Fourth International Conference, Vienna, Austria, 2–8 September 1973 by Hösli P. (1974)
10.1007/978-1-4757-1433-3_60 / Adv. exp. Med. Biol. / Development of a micro HG‐PRT activity assay: preliminary complementation studies with Lesch‐Nyhan cell strains by Hösli P. (1973)
10.1083/jcb.9.2.409
10.1083/jcb.36.1.231
10.1083/jcb.12.1.195
10.1111/j.1365-2818.1973.tb03794.x
10.1016/0014-4827(72)90161-9
{'key': 'e_1_2_1_24_1', 'volume-title': 'Cell and Tissue Culture', 'author': 'Paul J.', 'year': '1970'} / Cell and Tissue Culture by Paul J. (1970)
10.3109/10520296509116385 / Stain Technol. / Sheet mica, a nonadherent carrier for surface culture of cells to be embedded in epon by Persijn J.P. (1965)
10.1016/0014-4827(67)90278-9
10.3109/10520297309116616
10.1083/jcb.20.2.356
10.3109/10520296209117735 / Stain Technol. / Cover‐glass embedding in open‐end capsules for electron microscopy by Rosen S.J. (1962)
10.1007/BF02628264
{'key': 'e_1_2_1_31_1', 'first-page': '501', 'article-title': 'Fibroblasts in tissue culture. Use of colloidal iron for ultrastructural localization of acid mucopolysaccharides', 'volume': '14', 'author': 'Yardley J.H.', 'year': '1965', 'journal-title': 'Lab. Invest.'} / Lab. Invest. / Fibroblasts in tissue culture. Use of colloidal iron for ultrastructural localization of acid mucopolysaccharides by Yardley J.H. (1965)
{'key': 'e_1_2_1_32_1', 'first-page': '1458', 'article-title': "Méthode d'étude de cultures de cellules par la microscopie électronique: la culture sur support d'épon", 'volume': '262', 'author': 'Zagury D.', 'year': '1966', 'journal-title': 'C.r. Acad. Sci. (Paris)'} / C.r. Acad. Sci. (Paris) / Méthode d'étude de cultures de cellules par la microscopie électronique: la culture sur support d'épon by Zagury D. (1966)
{'key': 'e_1_2_1_33_1', 'first-page': '287', 'article-title': 'Preparation of cell monolayers for combined light and electron microscopy: staining in blocks', 'volume': '7', 'author': 'Zagury D.', 'year': '1968', 'journal-title': 'J. Microscopie'} / J. Microscopie / Preparation of cell monolayers for combined light and electron microscopy: staining in blocks by Zagury D. (1968)

Dates

Type	When
Created	14 years ago (Aug. 2, 2011, 12:51 p.m.)
Deposited	1 year, 10 months ago (Oct. 6, 2023, 2:05 p.m.)
Indexed	1 year, 9 months ago (Nov. 10, 2023, 8:20 p.m.)
Issued	49 years, 11 months ago (Sept. 1, 1975)
Published	49 years, 11 months ago (Sept. 1, 1975)
Published Online	14 years ago (Aug. 2, 2011)
Published Print	49 years, 11 months ago (Sept. 1, 1975)

Funders 0

None

BibTeX

@article{van_Ewijk_1975, title={A new method for comparative light and electron microscopic studies of individual cells, selected in the living state}, volume={105}, ISSN={1365-2818}, url={http://dx.doi.org/10.1111/j.1365-2818.1975.tb04034.x}, DOI={10.1111/j.1365-2818.1975.tb04034.x}, number={1}, journal={Journal of Microscopy}, publisher={Wiley}, author={van Ewijk, W. and Hösli, P.}, year={1975}, month=sep, pages={19–31} }

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