DOI: 10.1111/j.1365-2818.1975.tb04022.x. Changes of particle frequency in freeze‐etched erythrocyte membranes after fixation

Changes of particle frequency in freeze‐etched erythrocyte membranes after fixation

10.1111/j.1365-2818.1975.tb04022.x

Crossref journal-article

Wiley

Journal of Microscopy (311)

Abstract

SUMMARYThe frequency of particles on the membrane fracture faces of freeze‐etched human erythrocytes was measured, and the effect of fixation procedures on the particle frequencies was studied. Fresh blood, buffer washed cells and cells fixed in one of the following ways were examined: glutaraldehyde, glutaraldehyde followed by osmium tetroxide, osmium tetroxide alone. Quantitative analyses showed that some treatments produced a significant reduction in the number of particles on the fracture faces as compared with the fresh cells. After both osmium tetroxide fixations, the loss of particles was greater from the outer fracture face (OFF) than the inner fracture face (IFF), whilst after the other treatments approximately the same number of particles were lost from both fracture faces. The results are discussed with respect to some current concepts of the molecular architecture of the erythrocyte membrane and the action of fixatives. The reduction of particle frequencies is thought to be due to both leaching of membrane proteins, and deviations of the usual fracture plane within the membrane. Glutaraldehyde alone was shown to have less effect on particle frequency than the other fixatives and it is therefore a suitable fixative for the preparation of freeze‐etch specimens.

Bibliography

Parish, G. R. (1975). Changes of particle frequency in freezeâetched erythrocyte membranes after fixation. Journal of Microscopy, 104(3), 245â256. Portico.

Authors 1

G. R. Parish (first)

References 35 Referenced 27

10.1098/rstb.1971.0043
{'key': 'e_1_2_1_3_1', 'first-page': '107', 'volume-title': 'Freeze‐etching techniques and applications', 'author': 'Branton D.', 'year': '1973'} / Freeze‐etching techniques and applications by Branton D. (1973)
10.1126/science.181.4100.622
10.1007/BF00387320
10.1016/0047-7206(69)90006-5
10.1161/01.RES.22.3.405
10.1126/science.179.4069.190
{'key': 'e_1_2_1_9_1', 'first-page': '252', 'article-title': 'Localization of glycoproteins within erythrocyte membranes of sheep. A freeze‐etching and biochemical study', 'volume': '352', 'author': 'Pauli G.', 'year': '1974', 'journal-title': 'B. B. A.'} / B. B. A. / Localization of glycoproteins within erythrocyte membranes of sheep. A freeze‐etching and biochemical study by Pauli G. (1974)
10.1016/0005-2736(71)90292-6
10.1007/BF00306171
10.1007/BF01005005
10.1016/0005-2736(71)90150-7
10.1016/0022-2836(73)90394-X
10.1016/0304-4157(73)90013-0
10.1083/jcb.37.1.117
10.1073/pnas.70.11.3060
10.1111/j.1365-2818.1974.tb03963.x
10.1083/jcb.57.2.373
10.1083/jcb.59.2.395
{'key': 'e_1_2_1_21_1', 'first-page': '131', 'article-title': 'Seasonal variation in the membrane structure of differentiating shoot cambial‐zone cells demonstrated by freeze‐etching', 'volume': '9', 'author': 'Parish G.R.', 'year': '1974', 'journal-title': 'Cytobiol.'} / Cytobiol. / Seasonal variation in the membrane structure of differentiating shoot cambial‐zone cells demonstrated by freeze‐etching by Parish G.R. (1974)
10.1016/0005-2736(74)90071-6
{'key': 'e_1_2_1_23_1', 'volume-title': 'Hemolysis and related phenomena', 'author': 'Ponder E.C.', 'year': '1948'} / Hemolysis and related phenomena by Ponder E.C. (1948)
10.1002/jcp.1030610306
10.1177/17.7.482
10.1073/pnas.71.8.3294
10.3109/10520296809115036 / Stain Technol. / Embedding free‐floating cells and microscopic particles; serum albumin coagulum‐epoxy resin by Shands J.W. (1968)
10.1016/0005-2736(72)90037-5
10.1083/jcb.62.1.1
10.1084/jem.135.6.1209
10.1016/0005-2736(74)90208-9
10.1016/0005-2736(73)90143-0
10.1016/0304-4157(72)90019-6
{'key': 'e_1_2_1_34_1', 'first-page': '36', 'volume-title': 'Red Cell membrane structure and function', 'author': 'Weinstein R.S.', 'year': '1969'} / Red Cell membrane structure and function by Weinstein R.S. (1969)
{'key': 'e_1_2_1_35_1', 'first-page': '259', 'article-title': 'Ultrastructure of red cell membranes', 'volume': '7', 'author': 'Weinstein R.S.', 'year': '1970', 'journal-title': 'Seminars in Hematology'} / Seminars in Hematology / Ultrastructure of red cell membranes by Weinstein R.S. (1970)
10.1016/0304-4157(73)90003-8

Dates

Type	When
Created	14 years ago (Aug. 2, 2011, 12:12 p.m.)
Deposited	1 year, 9 months ago (Nov. 10, 2023, 11:06 a.m.)
Indexed	1 year, 1 month ago (July 10, 2024, 6:30 a.m.)
Issued	50 years ago (Aug. 1, 1975)
Published	50 years ago (Aug. 1, 1975)
Published Online	14 years ago (Aug. 2, 2011)
Published Print	50 years ago (Aug. 1, 1975)

Funders 0

None

BibTeX

@article{Parish_1975, title={Changes of particle frequency in freeze‐etched erythrocyte membranes after fixation}, volume={104}, ISSN={1365-2818}, url={http://dx.doi.org/10.1111/j.1365-2818.1975.tb04022.x}, DOI={10.1111/j.1365-2818.1975.tb04022.x}, number={3}, journal={Journal of Microscopy}, publisher={Wiley}, author={Parish, G. R.}, year={1975}, month=aug, pages={245–256} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        7,
        10
      ]
    ],
    "date-time": "2024-07-10T10:30:18Z",
    "timestamp": 1720607418264
  },
  "reference-count": 35,
  "publisher": "Wiley",
  "issue": "3",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2011,
            8,
            2
          ]
        ],
        "date-time": "2011-08-02T00:00:00Z",
        "timestamp": 1312243200000
      },
      "content-version": "vor",
      "delay-in-days": 13150,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [
      "onlinelibrary.wiley.com"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1975,
        8
      ]
    ]
  },
  "abstract": "<jats:title>SUMMARY</jats:title><jats:p>The frequency of particles on the membrane fracture faces of freeze\u2010etched human erythrocytes was measured, and the effect of fixation procedures on the particle frequencies was studied. Fresh blood, buffer washed cells and cells fixed in one of the following ways were examined: glutaraldehyde, glutaraldehyde followed by osmium tetroxide, osmium tetroxide alone. Quantitative analyses showed that some treatments produced a significant reduction in the number of particles on the fracture faces as compared with the fresh cells. After both osmium tetroxide fixations, the loss of particles was greater from the outer fracture face (OFF) than the inner fracture face (IFF), whilst after the other treatments approximately the same number of particles were lost from both fracture faces. The results are discussed with respect to some current concepts of the molecular architecture of the erythrocyte membrane and the action of fixatives. The reduction of particle frequencies is thought to be due to both leaching of membrane proteins, and deviations of the usual fracture plane within the membrane. Glutaraldehyde alone was shown to have less effect on particle frequency than the other fixatives and it is therefore a suitable fixative for the preparation of freeze\u2010etch specimens.</jats:p>",
  "DOI": "10.1111/j.1365-2818.1975.tb04022.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2011,
        8,
        2
      ]
    ],
    "date-time": "2011-08-02T16:12:48Z",
    "timestamp": 1312301568000
  },
  "page": "245-256",
  "update-policy": "http://dx.doi.org/10.1002/crossmark_policy",
  "source": "Crossref",
  "is-referenced-by-count": 27,
  "title": "Changes of particle frequency in freeze\u2010etched erythrocyte membranes after fixation",
  "prefix": "10.1111",
  "volume": "104",
  "author": [
    {
      "given": "G. R.",
      "family": "Parish",
      "sequence": "first",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2011,
        8,
        2
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1098/rstb.1971.0043"
    },
    {
      "key": "e_1_2_1_3_1",
      "first-page": "107",
      "volume-title": "Freeze\u2010etching techniques and applications",
      "author": "Branton D.",
      "year": "1973"
    },
    {
      "key": "e_1_2_1_4_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.181.4100.622"
    },
    {
      "key": "e_1_2_1_5_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00387320"
    },
    {
      "key": "e_1_2_1_6_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0047-7206(69)90006-5"
    },
    {
      "key": "e_1_2_1_7_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1161/01.RES.22.3.405"
    },
    {
      "key": "e_1_2_1_8_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.179.4069.190"
    },
    {
      "key": "e_1_2_1_9_1",
      "first-page": "252",
      "article-title": "Localization of glycoproteins within erythrocyte membranes of sheep. A freeze\u2010etching and biochemical study",
      "volume": "352",
      "author": "Pauli G.",
      "year": "1974",
      "journal-title": "B. B. A."
    },
    {
      "key": "e_1_2_1_10_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2736(71)90292-6"
    },
    {
      "key": "e_1_2_1_11_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00306171"
    },
    {
      "key": "e_1_2_1_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF01005005"
    },
    {
      "key": "e_1_2_1_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2736(71)90150-7"
    },
    {
      "key": "e_1_2_1_14_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0022-2836(73)90394-X"
    },
    {
      "key": "e_1_2_1_15_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-4157(73)90013-0"
    },
    {
      "key": "e_1_2_1_16_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.37.1.117"
    },
    {
      "key": "e_1_2_1_17_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.70.11.3060"
    },
    {
      "key": "e_1_2_1_18_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2818.1974.tb03963.x"
    },
    {
      "key": "e_1_2_1_19_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.57.2.373"
    },
    {
      "key": "e_1_2_1_20_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.59.2.395"
    },
    {
      "key": "e_1_2_1_21_1",
      "first-page": "131",
      "article-title": "Seasonal variation in the membrane structure of differentiating shoot cambial\u2010zone cells demonstrated by freeze\u2010etching",
      "volume": "9",
      "author": "Parish G.R.",
      "year": "1974",
      "journal-title": "Cytobiol."
    },
    {
      "key": "e_1_2_1_22_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2736(74)90071-6"
    },
    {
      "key": "e_1_2_1_23_1",
      "volume-title": "Hemolysis and related phenomena",
      "author": "Ponder E.C.",
      "year": "1948"
    },
    {
      "key": "e_1_2_1_24_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jcp.1030610306"
    },
    {
      "key": "e_1_2_1_25_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1177/17.7.482"
    },
    {
      "key": "e_1_2_1_26_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.71.8.3294"
    },
    {
      "key": "e_1_2_1_27_1",
      "doi-asserted-by": "crossref",
      "first-page": "15",
      "DOI": "10.3109/10520296809115036",
      "article-title": "Embedding free\u2010floating cells and microscopic particles; serum albumin coagulum\u2010epoxy resin",
      "volume": "43",
      "author": "Shands J.W.",
      "year": "1968",
      "journal-title": "Stain Technol."
    },
    {
      "key": "e_1_2_1_28_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2736(72)90037-5"
    },
    {
      "key": "e_1_2_1_29_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.62.1.1"
    },
    {
      "key": "e_1_2_1_30_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1084/jem.135.6.1209"
    },
    {
      "key": "e_1_2_1_31_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2736(74)90208-9"
    },
    {
      "key": "e_1_2_1_32_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2736(73)90143-0"
    },
    {
      "key": "e_1_2_1_33_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-4157(72)90019-6"
    },
    {
      "key": "e_1_2_1_34_1",
      "first-page": "36",
      "volume-title": "Red Cell membrane structure and function",
      "author": "Weinstein R.S.",
      "year": "1969"
    },
    {
      "key": "e_1_2_1_35_1",
      "first-page": "259",
      "article-title": "Ultrastructure of red cell membranes",
      "volume": "7",
      "author": "Weinstein R.S.",
      "year": "1970",
      "journal-title": "Seminars in Hematology"
    },
    {
      "key": "e_1_2_1_36_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-4157(73)90003-8"
    }
  ],
  "container-title": "Journal of Microscopy",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2818.1975.tb04022.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2818.1975.tb04022.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        11,
        10
      ]
    ],
    "date-time": "2023-11-10T16:06:01Z",
    "timestamp": 1699632361000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1111/j.1365-2818.1975.tb04022.x"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1975,
        8
      ]
    ]
  },
  "references-count": 35,
  "journal-issue": {
    "issue": "3",
    "published-print": {
      "date-parts": [
        [
          1975,
          8
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1111/j.1365-2818.1975.tb04022.x"
  ],
  "URL": "http://dx.doi.org/10.1111/j.1365-2818.1975.tb04022.x",
  "relation": {},
  "ISSN": [
    "0022-2720",
    "1365-2818"
  ],
  "subject": [],
  "container-title-short": "Journal of Microscopy",
  "published": {
    "date-parts": [
      [
        1975,
        8
      ]
    ]
  },
  "assertion": [
    {
      "value": "2011-08-02",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}