DOI: 10.1111/j.1365-2818.1969.tb00645.x. Electron microscopy of lipids: Effects of pH and fixatives on the appearance of a macromolecular assembly of lipid micelles in negatively stained preparations

Electron microscopy of lipids: Effects of pH and fixatives on the appearance of a macromolecular assembly of lipid micelles in negatively stained preparations

10.1111/j.1365-2818.1969.tb00645.x

Crossref journal-article

Wiley

Journal of Microscopy (311)

Abstract

SUMMARYNegatively stained preparations of macromolecular assemblies composed of lecithin, cholesterol and saponin have been examined by electron microscopy. Studies were made of the effects of different negative stains, pH and fixatives on the self‐assembly process that gives rise to helical and related structures, on the characteristic appearance of the structures that are formed, and on the stability of the different assemblies. A variety of different macromolecular assemblies, each apparently made of globular lipid subunits arranged in different ways, were observed under different experimental conditions. The structural appearance of the assemblies changes rapidly when the chemical constitution of the aqueous environment is altered. It is concluded that the negative‐staining technique normally provides an image of the hydrated specimen so that drying artifacts are not a major hazard, but the possibility of interactions between the specimen and the aqueous negative‐staining solution must not be ignored. The observations reported in this paper are relevant to an understanding of the factors that may control the formation, in vivo, of those biological membranes that contain discrete subunits composed of lipids or lipoproteins.

Bibliography

Glauert, A. M., & Lucy, J. A. (1969). Electron microscopy of lipids: Effects of pH and fixatives on the appearance of a macromolecular assembly of lipid micelles in negatively stained preparations. Journal of Microscopy, 89(1), 1â18. Portico.

Authors 2

Audrey M. Glauert (first)
J. A. Lucy (additional)

References 31 Referenced 27

10.1016/S0022-2836(64)80026-7
10.1177/6.5.303
10.1016/S0022-2836(64)80115-7
10.1016/S0022-2836(65)80094-8
10.1016/S0022-5320(67)80065-0
10.1016/0042-6822(61)90237-9
10.1016/0006-3002(59)90237-9
10.1042/bj0680352
10.1038/1941116a0
10.1083/jcb.24.1.23
10.1038/1971326a0
{'key': 'e_1_2_1_13_1', 'first-page': '166', 'volume-title': 'Techniques for Electron Microscopy', 'author': 'Glauert A.M.', 'year': '1965'} / Techniques for Electron Microscopy by Glauert A.M. (1965)
{'key': 'e_1_2_1_14_1', 'first-page': '1069', 'article-title': 'Factors influencing the appearance of biologic specimens in negatively stained preparations', 'volume': '14', 'author': 'Glauert A.M.', 'year': '1965', 'journal-title': 'Lab. Invest'} / Lab. Invest / Factors influencing the appearance of biologic specimens in negatively stained preparations by Glauert A.M. (1965)
{'key': 'e_1_2_1_15_1', 'first-page': '208', 'article-title': 'Electron microscopy of negatively‐stained lipids', 'volume': '63', 'author': 'Glauert A.M.', 'year': '1967', 'journal-title': 'Prow-plasma'} / Prow-plasma / Electron microscopy of negatively‐stained lipids by Glauert A.M. (1967)
10.1016/S0022-2275(20)39239-7 / J. Lipid Res / Role of lipids in the structure and function of biological membranes by Green D.E. (1966)
10.1016/0022-5193(63)90007-9
10.1007/BF00406937
10.1038/197822a0
{'key': 'e_1_2_1_20_1', 'first-page': '260', 'volume-title': 'Proceedings of the 1st Regional Conference on Electron Microscopy, Stockholm', 'author': 'Huxley H.E.', 'year': '1956'} / Proceedings of the 1st Regional Conference on Electron Microscopy, Stockholm by Huxley H.E. (1956)
10.1016/S0022-2836(60)80003-4
10.1016/0022-2836(66)90070-2
10.1016/0022-5193(64)90080-3
10.1016/S0022-2836(64)80121-2
{'key': 'e_1_2_1_25_1', 'first-page': '19', 'article-title': 'Assembly of macromolecular lipid structures in vitro', 'volume': '6', 'author': 'Lucy J.A.', 'year': '1968', 'journal-title': 'Symp. int. Soc. Cell Biol'} / Symp. int. Soc. Cell Biol / Assembly of macromolecular lipid structures in vitro by Lucy J.A. (1968)
10.1515/znb-1958-0908 / Z. Naturf / Etude au microscope électronique de plasmas contenant de l'acide désoxyribonucléique by Ryter A. (1958)
10.1083/jcb.17.1.19
10.1016/S0022-2836(64)80195-9
10.1016/0012-1606(63)90141-6
{'key': 'e_1_2_1_30_1', 'first-page': '539', 'article-title': "Ultrastructure du virus Sendai; influence des conditions de préparation sur l'aspect des particules viralcs examinées en coloration négative", 'volume': '2', 'author': 'Thiéry J.P.', 'year': '1963', 'journal-title': 'J. Microscopie'} / J. Microscopie / Ultrastructure du virus Sendai; influence des conditions de préparation sur l'aspect des particules viralcs examinées en coloration négative by Thiéry J.P. (1963)
10.1016/S0022-2836(62)80110-7
Zetterqvist H.(1956)The ultrastructural organization of the columnar absorbing cells of the mouse jejunum.Thesis Karolinska Institute Stockholm.

Dates

Type	When
Created	14 years ago (Aug. 2, 2011, 3:50 p.m.)
Deposited	1 year, 9 months ago (Nov. 10, 2023, 2:48 p.m.)
Indexed	1 year, 2 months ago (June 24, 2024, 10:14 p.m.)
Issued	56 years, 6 months ago (Feb. 1, 1969)
Published	56 years, 6 months ago (Feb. 1, 1969)
Published Online	14 years ago (Aug. 2, 2011)
Published Print	56 years, 6 months ago (Feb. 1, 1969)

Funders 0

None

BibTeX

@article{Glauert_1969, title={Electron microscopy of lipids: Effects of pH and fixatives on the appearance of a macromolecular assembly of lipid micelles in negatively stained preparations}, volume={89}, ISSN={1365-2818}, url={http://dx.doi.org/10.1111/j.1365-2818.1969.tb00645.x}, DOI={10.1111/j.1365-2818.1969.tb00645.x}, number={1}, journal={Journal of Microscopy}, publisher={Wiley}, author={Glauert, Audrey M. and Lucy, J. A.}, year={1969}, month=feb, pages={1–18} }

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