DOI: 10.1111/j.1365-2818.1959.tb04474.x. SOME APPLICATIONS OF THE ULTRA‐VIOLET AND INTERFERENCE MICROSCOPES IN ELECTRON MICROSCOPY

SOME APPLICATIONS OF THE ULTRA‐VIOLET AND INTERFERENCE MICROSCOPES IN ELECTRON MICROSCOPY

10.1111/j.1365-2818.1959.tb04474.x

Crossref journal-article

Wiley

Journal of the Royal Microscopical Society (311)

Abstract

SYNOPSISAn ultra‐violet microscope of high resolution, using radiation of wavelength 2570 Å, is described. This makes it useful to the electron microscopist for giving information about the concentration of nucleic acids in different parts of an organism. Three different types of application are described. Firstly, a comparison of ultra‐violet pictures with electron micrographs of identical bacteria (Mycobacterium phlei); secondly, a comparison of light microscope pictures of Feulgen‐stained organisms (Streptomyces cœlicolor) with ultra‐violet pictures as well as with electron micrographs of these bacteria at the same stage of growth. The third application concerns a comparative study in the ultra‐violet and electron microscopes of locust testis in order to assist in interpreting the electron microscope picture of the nucleus in these cells.The interference microscope was used for measuring accurately the thickness and refractive index of thin sections. A special grid for mounting thin sections to be measured in the interference microscope was designed. The method was used to investigate the effect of the electron beam on thin sections of four different embedding materials (Methacrylate, Araldite, Vestopal, Aquon). It was found that all sections become thinner, the loss of thickness ranging from 25–60 per cent according to the nature of the material and the amount of irradiation.

Bibliography

Cosslett, A. (1959). SOME APPLICATIONS OF THE ULTRAâVIOLET AND INTERFERENCE MICROSCOPES IN ELECTRON MICROSCOPY. Journal of the Royal Microscopical Society, 79(3), 263â271. Portico.

Authors 1

Anna Cosslett (first)

References 14 Referenced 19

{'key': 'e_1_2_1_2_1', 'first-page': '301', 'article-title': 'An electron‐microscopical study of bull‐sperm, III', 'volume': '52', 'author': 'Bretschneider L. H.', 'year': '1949', 'journal-title': 'Proc. Acad. Sci. Amst.'} / Proc. Acad. Sci. Amst. / An electron‐microscopical study of bull‐sperm, III by Bretschneider L. H. (1949)
Gibbons I. R.(1957).“Electron microscopic studies on Locust testis”.Dissertation University of Cambridge.
Gibbons I. R.(1958).A water‐miscible embedding resin for electron microscopy. Proc. 4th Internat. Congr. Elec. Micr. Springer Berlin 55. (10.1007/978-3-662-22058-0_10)
10.1099/00221287-13-2-310
10.1083/jcb.7.3.479
10.1083/jcb.4.2.191
10.1083/jcb.8.1.267
{'key': 'e_1_2_1_9_1', 'first-page': '512', 'article-title': 'Elektronenmikroskopie', 'volume': '33', 'author': 'Leisegang S.', 'year': '1956', 'journal-title': 'Handb. Phys.'} / Handb. Phys. / Elektronenmikroskopie by Leisegang S. (1956)
10.6028/jres.043.021
10.1016/0014-4827(50)90046-2
10.1515/znb-1959-8-916 / Z. Naturf. / Quantitative Untersuchung zur Massenabnahme von Einbettungsmitteln (Methacrylat, Vestopal und Araldit) unter Elektronenbeschuss by Reimer L. (1959)
10.1016/S0022-5320(58)90018-2
10.1038/173362b0
{'key': 'e_1_2_1_15_1', 'first-page': '1', 'article-title': 'The living cell in vitro as shown by darkground illumination and the changes induced in such cells by fixing reagents', 'volume': '71', 'author': 'Strangeways T. S. P.', 'year': '1927', 'journal-title': 'Quart. J. micr. Sci.'} / Quart. J. micr. Sci. / The living cell in vitro as shown by darkground illumination and the changes induced in such cells by fixing reagents by Strangeways T. S. P. (1927)

Dates

Type	When
Created	13 years, 9 months ago (Nov. 11, 2011, 1:58 p.m.)
Deposited	1 year, 9 months ago (Nov. 10, 2023, 1:38 p.m.)
Indexed	1 year, 9 months ago (Nov. 10, 2023, 7:07 p.m.)
Issued	65 years, 11 months ago (Oct. 1, 1959)
Published	65 years, 11 months ago (Oct. 1, 1959)
Published Online	13 years, 9 months ago (Nov. 11, 2011)
Published Print	65 years, 11 months ago (Oct. 1, 1959)

Funders 0

None

BibTeX

@article{Cosslett_1959, title={SOME APPLICATIONS OF THE ULTRA‐VIOLET AND INTERFERENCE MICROSCOPES IN ELECTRON MICROSCOPY}, volume={79}, ISSN={0368-3974}, url={http://dx.doi.org/10.1111/j.1365-2818.1959.tb04474.x}, DOI={10.1111/j.1365-2818.1959.tb04474.x}, number={3}, journal={Journal of the Royal Microscopical Society}, publisher={Wiley}, author={Cosslett, Anna}, year={1959}, month=oct, pages={263–271} }

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