DOI: 10.1111/j.1365-2672.1988.tb02444.x. Mechanism of resistance of <i>Bacillus subtilis</i> spores to chlorhexidine

Mechanism of resistance of Bacillus subtilis spores to chlorhexidine

10.1111/j.1365-2672.1988.tb02444.x

Crossref journal-article

Wiley

Journal of Applied Bacteriology (311)

Abstract

Chlorhexidine diacetate (CHA) was rather more sporicidal at 20C to ureadithreitol‐sodium lauryl sulphate (UDS)‐treated spores of Bacillus subtilis NCTC 8236 than to urea‐dithiothreitol (UDT)‐treated or normal (untreated) spores. UDS spores adsorbed more CHA from solution than did the other two forms. No differences in hydrophobicity, as determined by hydrophobic interaction chromatography (HIC) or bacterial adherence to hydrocarbon (BATH), could be detected between the three spore types. Germinating spores took up much less CHA than did outgrowing spores. Germinating cells were considerably more hydrophobic, as measured by the BATH technique, than outgrowing cells or normal spores. Chlorhexidine diacetate increased the apparent hydrophobicity of the two latter forms, but this effect could be partially reversed by subsequent exposure to a non‐ionic surfactant.

Bibliography

Shaker, L. A., Furr, J. R., & Russell, A. D. (1988). Mechanism of resistance of Bacillus subtilis spores to chlorhexidine. Journal of Applied Bacteriology, 64(6), 531â539. Portico.

Authors 3

L.A. Shaker (first)
J.R. Furr (additional)
A.D. Russell (additional)

References 24 Referenced 15

10.1111/j.1365-2672.1965.tb02160.x
10.1007/BF01626560
10.1111/j.1472-765X.1985.tb01480.x
10.1093/jac/16.6.685
10.1016/0378-5173(83)90040-6
10.1111/j.1365-2672.1984.tb01350.x
10.1111/j.1365-2672.1962.tb01116.x
{'key': 'e_1_2_1_9_1', 'first-page': '158', 'volume-title': 'Principles and Practice of Disinfection, Preservation and Sterilisation', 'author': 'Hugo W.B.', 'year': '1982'} / Principles and Practice of Disinfection, Preservation and Sterilisation by Hugo W.B. (1982)
{'key': 'e_1_2_1_10_1', 'first-page': '8', 'volume-title': 'Principles and Practice of Disinfection, Preservation and Sterilisation', 'author': 'Hugo W.B.', 'year': '1982'} / Principles and Practice of Disinfection, Preservation and Sterilisation by Hugo W.B. (1982)
10.1111/j.1365-2672.1986.tb04299.x
10.1007/BF01573707
10.1128/iai.38.2.637-644.1982 / Infection and Immunity / Hydrophobic interactions and the adhesion of Streptococcus sanguis to hydroxyapatite by Nesbitt W.E. (1982)
10.1083/jcb.17.1.208
10.1007/BF00409236
{'key': 'e_1_2_1_16_1', 'volume-title': 'The Destruction of Bacterial Spores', 'author': 'Russell A.D.', 'year': '1982'} / The Destruction of Bacterial Spores by Russell A.D. (1982)
10.1016/0378-5173(83)90050-9
10.1007/BF01644264
10.1016/0378-5173(85)90108-5
10.1016/0378-5173(86)90009-8
10.1128/iai.22.2.462-472.1978 / Infection and Immunity / Differences in hydrophobic surface characteristics of porcine enteropathogenic Escherichia coli with or without K88 antigen as revealed by hydrophobic interaction chromatography by Smyth G.J. (1978)
10.1128/aem.50.2.298-303.1985 / Applied and Environmental Microbiology / Destruction of the outer membrane permeability barrier of Escherichia coli by heat treatment by Tsuchido T. (1985)
{'key': 'e_1_2_1_23_1', 'first-page': '195', 'article-title': 'Antifungal action of chlorhexidine', 'volume': '38', 'author': 'Walters T.H.', 'year': '1983', 'journal-title': 'Microbios'} / Microbios / Antifungal action of chlorhexidine by Walters T.H. (1983)
{'key': 'e_1_2_1_24_1', 'first-page': '207', 'volume-title': 'Principles and Practice of Disinfection, Preservation and Sterilisation', 'author': 'Waites W.', 'year': '1982'} / Principles and Practice of Disinfection, Preservation and Sterilisation by Waites W. (1982)
{'key': 'e_1_2_1_25_1', 'first-page': '383', 'volume-title': 'Fundamental and Applied Aspects of Bacterial Spores', 'author': 'Waites W.', 'year': '1985'} / Fundamental and Applied Aspects of Bacterial Spores by Waites W. (1985)

Dates

Type	When
Created	17 years, 5 months ago (March 11, 2008, 7:25 a.m.)
Deposited	1 year, 10 months ago (Oct. 20, 2023, 7:38 p.m.)
Indexed	1 year, 10 months ago (Oct. 25, 2023, 2:59 p.m.)
Issued	37 years, 3 months ago (June 1, 1988)
Published	37 years, 3 months ago (June 1, 1988)
Published Online	17 years, 5 months ago (March 11, 2008)
Published Print	37 years, 3 months ago (June 1, 1988)

Funders 0

None

BibTeX

@article{Shaker_1988, title={Mechanism of resistance of Bacillus subtilis spores to chlorhexidine}, volume={64}, ISSN={0021-8847}, url={http://dx.doi.org/10.1111/j.1365-2672.1988.tb02444.x}, DOI={10.1111/j.1365-2672.1988.tb02444.x}, number={6}, journal={Journal of Applied Bacteriology}, publisher={Wiley}, author={Shaker, L.A. and Furr, J.R. and Russell, A.D.}, year={1988}, month=jun, pages={531–539} }

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