DOI: 10.1111/j.1365-2559.1995.tb00618.x. Keratin expression in the normal anal canal

Keratin expression in the normal anal canal

10.1111/j.1365-2559.1995.tb00618.x

Crossref journal-article

Wiley

Histopathology (311)

Abstract

The pattern of epithelial keratin expression in the normal anal canal has not been extensively defined and is a necessary prerequisite to the interpretation of alterations in these intermediate filaments in pathological anal epithelial lesions. Thirty‐five frozen tissue specimens of resected haemorrhoids were investigated immunohistolo‐gically for expression of 14 individual keratins (K) using a panel of 17 monoclonal antibodies. Perianal skin showed basal expression of karatinocyte Ks 5, 14 and 17, and suprabasal expression of keratinocyte Ks 14, 10, 1 and 16. Anal squamous epithelium showed persistent basal K5 and 17, basal and suprabasal K4, 13 and 16 positivity, with sporadic expression of K1 and 10. The expression of simple epithelial keratins in squamous epithelium adjacent to the anal transitional zone varied with basal expression of K7, K8, K18 and K19 and sporadic suprabasal expression of K7 and K19. The anal transitional zone (ATZ) expressed K19, as found in transitional epithelia elsewhere. The full thickness of epithelium was positive for the simple epithelial Ks 7, 8 18 and 19. Marked heterogeneity of keratinocyte keratin expression was seen. Basal layers expressed Ks 4, 13, 14 and 17 and variably K16, while suprabasal layers expressed Ks4 and 13, 14 and 17 and variably K16, while suprabasal layers expressed Ks4 and 13 and variably K14, 16 and 17. This anomalous expression of keratinocyte K4 and 13 has also been documented in transitional epithelium of the bladder. The anal glands and ducts showed a keratin distribution similar to the transition zone. Rectal columnar epithelium expressed simple keratins 7, 8 18 and 19. In addition, low levels of keratinocyte keratins were found as indicated by heterogeneous staining for K4, 13, 14 and 16. The overall pattern, particularly in the region of the anal transitional zone and immediately adjacent squamous and columnar epithelia, is of a flexible epithelial cell population able to express a range of keratins unrestricted by a particular morphological phenotype. In the light of these results, analysis of changes in keratin distribution within anal carcinomas may assist classification by providing information on the state of differentiation and histogenesis of these tumours.

Bibliography

WILLIAMS, G. R., TALBOT, I. C., NORTHOVER, J. M. A., & LEIGH, I. M. (1995). Keratin expression in the normal anal canal. Histopathology, 26(1), 39â44. Portico.

Authors 4

G.R. WILLIAMS (first)
I.C. TALBOT (additional)
J.M.A. NORTHOVER (additional)
I.M. LEIGH (additional)

References 14 Referenced 14

10.1016/0092-8674(82)90400-7
{'key': 'e_1_2_1_3_2', 'first-page': '243', 'article-title': 'Classification of human epithelia and their neoplasms using monoclonal antibodies to keratins: strategies, applications and limitations.', 'volume': '52', 'author': 'Cooper D', 'year': '1985', 'journal-title': 'Lab. Invest.'} / Lab. Invest. / Classification of human epithelia and their neoplasms using monoclonal antibodies to keratins: strategies, applications and limitations. by Cooper D (1985)
10.1016/0092-8674(82)90234-3
10.1111/j.1749-6632.1985.tb50419.x
{'key': 'e_1_2_1_6_2', 'first-page': '165', 'article-title': 'Use of keratin antibodies in tumor diagnosis.', 'volume': '1', 'author': 'Lane EB', 'year': '1990', 'journal-title': 'Semin. Cancer. Biol.'} / Semin. Cancer. Biol. / Use of keratin antibodies in tumor diagnosis. by Lane EB (1990)
10.1242/jcs.93.3.419 / J. Cell Sci. / Patterns of expression of keratin 17 in human epithelia: dependence on cell position. by Troyanovsky SM (1989)
10.1007/BF01605932
10.1007/BF00492389
10.1111/j.1432-0436.1986.tb00400.x
10.1083/jcb.106.5.1635
10.1007/978-3-642-83693-0
10.1242/jcs.94.3.403 / J. Cell Sci. / Keratin expression in human mammary epithelial cells cultured from normal and malignant tissue: relation to in vivo phenotypes and influence of medium. by Taylor‐Papadimitriou J (1989)
{'key': 'e_1_2_1_14_2', 'first-page': '416', 'article-title': 'The pathology and results of treatment of squamous cell carcinoma of the anal canal and anal margin.', 'volume': '53', 'author': 'Morson BC', 'year': '1960', 'journal-title': 'Prog. Roy. Soc. Med.'} / Prog. Roy. Soc. Med. / The pathology and results of treatment of squamous cell carcinoma of the anal canal and anal margin. by Morson BC (1960)
10.1056/NEJM196403192701205

Dates

Type	When
Created	18 years, 5 months ago (April 3, 2007, 10:36 a.m.)
Deposited	1 year, 10 months ago (Oct. 26, 2023, 10:22 a.m.)
Indexed	1 year, 10 months ago (Oct. 27, 2023, 1:10 a.m.)
Issued	30 years, 8 months ago (Jan. 1, 1995)
Published	30 years, 8 months ago (Jan. 1, 1995)
Published Online	18 years, 5 months ago (April 3, 2007)
Published Print	30 years, 8 months ago (Jan. 1, 1995)

Funders 0

None

BibTeX

@article{WILLIAMS_1995, title={Keratin expression in the normal anal canal}, volume={26}, ISSN={1365-2559}, url={http://dx.doi.org/10.1111/j.1365-2559.1995.tb00618.x}, DOI={10.1111/j.1365-2559.1995.tb00618.x}, number={1}, journal={Histopathology}, publisher={Wiley}, author={WILLIAMS, G.R. and TALBOT, I.C. and NORTHOVER, J.M.A. and LEIGH, I.M.}, year={1995}, month=jan, pages={39–44} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2023,
        10,
        27
      ]
    ],
    "date-time": "2023-10-27T05:10:44Z",
    "timestamp": 1698383444528
  },
  "reference-count": 14,
  "publisher": "Wiley",
  "issue": "1",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2007,
            4,
            3
          ]
        ],
        "date-time": "2007-04-03T00:00:00Z",
        "timestamp": 1175558400000
      },
      "content-version": "vor",
      "delay-in-days": 4475,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [
      "onlinelibrary.wiley.com"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1995,
        1
      ]
    ]
  },
  "abstract": "<jats:p>The pattern of epithelial keratin expression in the normal anal canal has not been extensively defined and is a necessary prerequisite to the interpretation of alterations in these intermediate filaments in pathological anal epithelial lesions. Thirty\u2010five frozen tissue specimens of resected haemorrhoids were investigated immunohistolo\u2010gically for expression of 14 individual keratins (K) using a panel of 17 monoclonal antibodies. <jats:italic>Perianal skin</jats:italic> showed basal expression of karatinocyte Ks 5, 14 and 17, and suprabasal expression of keratinocyte Ks 14, 10, 1 and 16. <jats:italic>Anal squamous epithelium</jats:italic> showed persistent basal K5 and 17, basal and suprabasal K4, 13 and 16 positivity, with sporadic expression of K1 and 10. The expression of simple epithelial keratins in squamous epithelium adjacent to the anal transitional zone varied with basal expression of K7, K8, K18 and K19 and sporadic suprabasal expression of K7 and K19. The <jats:italic>anal transitional zone (ATZ)</jats:italic> expressed K19, as found in transitional epithelia elsewhere. The full thickness of epithelium was positive for the simple epithelial Ks 7, 8 18 and 19. Marked heterogeneity of keratinocyte keratin expression was seen. Basal layers expressed Ks 4, 13, 14 and 17 and variably K16, while suprabasal layers expressed Ks4 and 13, 14 and 17 and variably K16, while suprabasal layers expressed Ks4 and 13 and variably K14, 16 and 17. This anomalous expression of keratinocyte K4 and 13 has also been documented in transitional epithelium of the bladder. The <jats:italic>anal glands and ducts</jats:italic> showed a keratin distribution similar to the transition zone. <jats:italic>Rectal columnar epithelium</jats:italic> expressed simple keratins 7, 8 18 and 19. In addition, low levels of keratinocyte keratins were found as indicated by heterogeneous staining for K4, 13, 14 and 16. The overall pattern, particularly in the region of the anal transitional zone and immediately adjacent squamous and columnar epithelia, is of a flexible epithelial cell population able to express a range of keratins unrestricted by a particular morphological phenotype. In the light of these results, analysis of changes in keratin distribution within anal carcinomas may assist classification by providing information on the state of differentiation and histogenesis of these tumours.</jats:p>",
  "DOI": "10.1111/j.1365-2559.1995.tb00618.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2007,
        4,
        3
      ]
    ],
    "date-time": "2007-04-03T14:36:35Z",
    "timestamp": 1175610995000
  },
  "page": "39-44",
  "update-policy": "http://dx.doi.org/10.1002/crossmark_policy",
  "source": "Crossref",
  "is-referenced-by-count": 14,
  "title": "Keratin expression in the normal anal canal",
  "prefix": "10.1111",
  "volume": "26",
  "author": [
    {
      "given": "G.R.",
      "family": "WILLIAMS",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "I.C.",
      "family": "TALBOT",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "J.M.A.",
      "family": "NORTHOVER",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "I.M.",
      "family": "LEIGH",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2007,
        4,
        3
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(82)90400-7"
    },
    {
      "key": "e_1_2_1_3_2",
      "first-page": "243",
      "article-title": "Classification of human epithelia and their neoplasms using monoclonal antibodies to keratins: strategies, applications and limitations.",
      "volume": "52",
      "author": "Cooper D",
      "year": "1985",
      "journal-title": "Lab. Invest."
    },
    {
      "key": "e_1_2_1_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(82)90234-3"
    },
    {
      "key": "e_1_2_1_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1749-6632.1985.tb50419.x"
    },
    {
      "key": "e_1_2_1_6_2",
      "first-page": "165",
      "article-title": "Use of keratin antibodies in tumor diagnosis.",
      "volume": "1",
      "author": "Lane EB",
      "year": "1990",
      "journal-title": "Semin. Cancer. Biol."
    },
    {
      "key": "e_1_2_1_7_2",
      "doi-asserted-by": "crossref",
      "first-page": "419",
      "DOI": "10.1242/jcs.93.3.419",
      "article-title": "Patterns of expression of keratin 17 in human epithelia: dependence on cell position.",
      "volume": "93",
      "author": "Troyanovsky SM",
      "year": "1989",
      "journal-title": "J. Cell Sci."
    },
    {
      "key": "e_1_2_1_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF01605932"
    },
    {
      "key": "e_1_2_1_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00492389"
    },
    {
      "key": "e_1_2_1_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-0436.1986.tb00400.x"
    },
    {
      "key": "e_1_2_1_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.106.5.1635"
    },
    {
      "key": "e_1_2_1_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/978-3-642-83693-0"
    },
    {
      "key": "e_1_2_1_13_2",
      "doi-asserted-by": "crossref",
      "first-page": "403",
      "DOI": "10.1242/jcs.94.3.403",
      "article-title": "Keratin expression in human mammary epithelial cells cultured from normal and malignant tissue: relation to in vivo phenotypes and influence of medium.",
      "volume": "94",
      "author": "Taylor\u2010Papadimitriou J",
      "year": "1989",
      "journal-title": "J. Cell Sci."
    },
    {
      "key": "e_1_2_1_14_2",
      "first-page": "416",
      "article-title": "The pathology and results of treatment of squamous cell carcinoma of the anal canal and anal margin.",
      "volume": "53",
      "author": "Morson BC",
      "year": "1960",
      "journal-title": "Prog. Roy. Soc. Med."
    },
    {
      "key": "e_1_2_1_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1056/NEJM196403192701205"
    }
  ],
  "container-title": "Histopathology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2559.1995.tb00618.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2559.1995.tb00618.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        10,
        26
      ]
    ],
    "date-time": "2023-10-26T14:22:04Z",
    "timestamp": 1698330124000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1111/j.1365-2559.1995.tb00618.x"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1995,
        1
      ]
    ]
  },
  "references-count": 14,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          1995,
          1
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1111/j.1365-2559.1995.tb00618.x"
  ],
  "URL": "http://dx.doi.org/10.1111/j.1365-2559.1995.tb00618.x",
  "relation": {},
  "ISSN": [
    "0309-0167",
    "1365-2559"
  ],
  "subject": [],
  "container-title-short": "Histopathology",
  "published": {
    "date-parts": [
      [
        1995,
        1
      ]
    ]
  },
  "assertion": [
    {
      "value": "2007-04-03",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}