DOI: 10.1111/j.1365-2443.2006.00936.x. Nucleocytoplasmic transport of fluorescent mRNA in living mammalian cells: nuclear mRNA export is coupled to ongoing gene transcription

Nucleocytoplasmic transport of fluorescent mRNA in living mammalian cells: nuclear mRNA export is coupled to ongoing gene transcription

10.1111/j.1365-2443.2006.00936.x

Crossref journal-article

Wiley

Genes to Cells (311)

Abstract

In eukaryotic cells, export of mRNA from the nucleus to the cytoplasm is one of the essential steps in gene expression. To examine mechanisms involved in the nucleocytoplasmic transport of mRNA, we microinjected fluorescently labeled fushi tarazu (ftz) pre‐mRNA into the nuclei of HeLa cells. The injected intron‐containing ftz pre‐mRNA was distributed to the SC35 speckles and exported to the cytoplasm after splicing by an energy‐requiring active process. In contrast, the injected intron‐less ftz mRNA was diffusely distributed in the nucleus and then presumably degraded. Interestingly, export of the ftz pre‐mRNA was inhibited by treatment with transcriptional inhibitors (actinomycin D, α‐amanitin or DRB). Cells treated with transcriptional inhibitor showed foci enriched with the injected mRNA, which localize side by side with SC35 speckles. Those nuclear foci, referred to as TIDRs (transcriptional‐inactivation dependent RNA domain), do not overlap with paraspeckles. In addition, in situ hybridization analysis revealed that the export of endogenous poly(A)+ mRNA is also affected by transcriptional inactivation. These results suggest that nuclear mRNA export is coupled to ongoing gene transcription in mammalian cells.

Bibliography

Tokunaga, K., Shibuya, T., Ishihama, Y., Tadakuma, H., Ide, M., Yoshida, M., Funatsu, T., Ohshima, Y., & Tani, T. (2006). Nucleocytoplasmic transport of fluorescent mRNA in living mammalian cells: nuclear mRNA export is coupled to ongoing gene transcription. Genes to Cells, 11(3), 305â317. Portico.

Authors 9

Kazuaki Tokunaga (first)
Toshiharu Shibuya (additional)
Yo Ishihama (additional)
Hisashi Tadakuma (additional)
Miyuki Ide (additional)
Minoru Yoshida (additional)
Takashi Funatsu (additional)
Yasumi Ohshima (additional)
Tokio Tani (additional)

References 38 Referenced 48

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Dates

Type	When
Created	19 years, 7 months ago (Jan. 12, 2006, 10:26 a.m.)
Deposited	1 year, 9 months ago (Oct. 31, 2023, 3:57 a.m.)
Indexed	4 months, 3 weeks ago (March 29, 2025, 9:48 p.m.)
Issued	19 years, 7 months ago (Jan. 20, 2006)
Published	19 years, 7 months ago (Jan. 20, 2006)
Published Online	19 years, 7 months ago (Jan. 20, 2006)
Published Print	19 years, 5 months ago (March 1, 2006)

Funders 0

None

BibTeX

@article{Tokunaga_2006, title={Nucleocytoplasmic transport of fluorescent mRNA in living mammalian cells: nuclear mRNA export is coupled to ongoing gene transcription}, volume={11}, ISSN={1365-2443}, url={http://dx.doi.org/10.1111/j.1365-2443.2006.00936.x}, DOI={10.1111/j.1365-2443.2006.00936.x}, number={3}, journal={Genes to Cells}, publisher={Wiley}, author={Tokunaga, Kazuaki and Shibuya, Toshiharu and Ishihama, Yo and Tadakuma, Hisashi and Ide, Miyuki and Yoshida, Minoru and Funatsu, Takashi and Ohshima, Yasumi and Tani, Tokio}, year={2006}, month=jan, pages={305–317} }

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