DOI: 10.1111/j.1365-2362.1972.tb00584.x. Isolation, Biological and Antigenic Properties of a Specific Toxin Formed in Thermally Altered Mouse Skin

Isolation, Biological and Antigenic Properties of a Specific Toxin Formed in Thermally Altered Mouse Skin

10.1111/j.1365-2362.1972.tb00584.x

Crossref journal-article

Wiley

European Journal of Clinical Investigation (311)

Abstract

Abstract. A lipid‐protein complex, which has a lethal effect on recipient animals, was isolated from mouse skin exposed to controlled thermal energy. A new isolation procedure was developed, which takes advantage of the toxic activity present in thermally modified skin, to trace the fate of the active compound. A bioassay was designed for this purpose by injecting Thorotrast into the recipient animals. The toxic lipid‐protein complex and the corresponding derivative from native skin were shown to have the same lipid (40%) and protein (60%) content. The lipid moieties of both derivatives were made up of six different lipid classes. The toxic compound differed from the non‐toxic material only by its larger size and higher density. Equivalent results were obtained on samples isolated, after the removal of surface lipids and soluble cell constituents, from the donor skins prior to the application of thermal energy i.e. processing “skin residues”. The biological activity was shown to reside in the apoprotein while the lipid moiety contributes to the toxic activity to a certain extent. The results suggest that the toxic compound is a polymeric form produced by thermal energy from a naturally occurring precursor. The toxin has a specific antigenic property protecting mice after active and passive immunotherapy against a lethal bum injury in vivo. This suggests strongly that the toxic compound is an etiological factor responsible for the high mortality after severe burns. It is significant that, if scalding was applied to the same extent as the dry heat burns in vivo, the injured animals survived. This was interpreted to support the hypothesis of a specific mechanism being responsible for toxin formation. The experimental results suggest that a specific therapy for the “late mortality” after severe human burns might be possible.

Bibliography

Schoenenberger, G. A., AllgÃ¶wer, M., Cueni, L. B., Eppenberger, U., & StÃ¤dtler, K. E. (1972). Isolation, Biological and Antigenic Properties of a Specific Toxin Formed in Thermally Altered Mouse Skin. European Journal of Clinical Investigation, 2(3), 154â162. Portico.

Authors 5

G. A. Schoenenberger (first)
M. Allgöwer (additional)
L. B. Cueni (additional)
U. Eppenberger (additional)
K. E. Städtler (additional)

References 23 Referenced 5

{'key': 'e_1_2_1_2_2', 'volume-title': 'Burns, shock and plasma volume regulation', 'author': 'Moyer C. A.', 'year': '1967'} / Burns, shock and plasma volume regulation by Moyer C. A. (1967)
{'key': 'e_1_2_1_3_2', 'first-page': '433', 'article-title': 'Recent and current trends in burn research and treatment', 'volume': '97', 'author': 'Kernahan D. D.', 'year': '1967', 'journal-title': 'Canad. med. Ass. J.'} / Canad. med. Ass. J. / Recent and current trends in burn research and treatment by Kernahan D. D. (1967)
{'key': 'e_1_2_1_4_2', 'first-page': '813', 'article-title': 'Probite analysis of burn death in a development', 'volume': '68', 'author': 'Ramirez A. T.', 'year': '1970', 'journal-title': 'Surgery'} / Surgery / Probite analysis of burn death in a development by Ramirez A. T. (1970)
10.1016/0022-4804(67)90075-3
{'key': 'e_1_2_1_6_2', 'first-page': '126', 'article-title': 'Toxaemia of burns', 'volume': '2', 'author': 'Sevitt S.', 'year': '1966', 'journal-title': 'Modern Trends in Plastic Surgery'} / Modern Trends in Plastic Surgery / Toxaemia of burns by Sevitt S. (1966)
10.1016/S0039-6109(16)39295-7
10.1111/j.1749-6632.1968.tb14732.x
10.1080/00039896.1965.10664247
{'key': 'e_1_2_1_10_2', 'article-title': 'Isolation of a toxic substance from mouse skin after severe burn', 'volume': '119', 'author': 'Schoenenberger G. A.', 'year': '1968', 'journal-title': 'Excerpta Media Internat.'} / Excerpta Media Internat. / Isolation of a toxic substance from mouse skin after severe burn by Schoenenberger G. A. (1968)
Allgöwer M. Cueni L. B. Donatsch P. Staudtler K. Schoenenberger G. A.:Burn toxins in mouse skin.J. Trauma(in press).
Cueni L. B. Allgöwer M. Schoenenberger G. A.:Isolierung und physikalisch‐chemische Charakterisierung eines Verbrennungstoxines aus der Mäusehaut.Z. ges. exp. Med.(in press).
{'key': 'e_1_2_1_13_2', 'first-page': '195', 'article-title': 'Chemical analysis of the lipid and protein fractions of an unknown burn toxin in mouse skin, and their biochemical role for occurrence of toxicity', 'volume': '1', 'author': 'Schoenenberger G. A.', 'year': '1969', 'journal-title': 'Europ. Surg. Res.'} / Europ. Surg. Res. / Chemical analysis of the lipid and protein fractions of an unknown burn toxin in mouse skin, and their biochemical role for occurrence of toxicity by Schoenenberger G. A. (1969)
{'key': 'e_1_2_1_14_2', 'volume-title': 'Thin layer chromatography', 'author': 'Stahl E.', 'year': '1965'} / Thin layer chromatography by Stahl E. (1965)
10.1038/jid.1969.62
{'key': 'e_1_2_1_16_2', 'first-page': '14', 'volume-title': 'Thin layer chromatography', 'author': 'Potter V. R.', 'year': '1965'} / Thin layer chromatography by Potter V. R. (1965)
10.1016/S0021-9258(18)64849-5
10.1111/j.1749-6632.1964.tb14207.x
10.1016/0005-2795(68)90076-7
10.1016/0003-2697(68)90173-5
10.1016/0006-291X(71)90526-2
{'key': 'e_1_2_1_22_2', 'first-page': '471', 'volume-title': 'Research in burns', 'author': 'Cueni L.', 'year': '1971'} / Research in burns by Cueni L. (1971)
{'key': 'e_1_2_1_23_2', 'first-page': '515', 'article-title': 'Isolation and characterization of a toxic lipoprotein derived from germ‐free mouse skin', 'volume': '21', 'author': 'Schoenenberger G. A.', 'year': '1970', 'journal-title': 'Surg. Forum'} / Surg. Forum / Isolation and characterization of a toxic lipoprotein derived from germ‐free mouse skin by Schoenenberger G. A. (1970)
10.1097/00000658-196812000-00008

Dates

Type	When
Created	17 years, 5 months ago (March 20, 2008, 10:39 a.m.)
Deposited	1 year, 9 months ago (Nov. 10, 2023, 12:21 p.m.)
Indexed	1 year, 9 months ago (Nov. 10, 2023, 7:34 p.m.)
Issued	53 years, 3 months ago (June 1, 1972)
Published	53 years, 3 months ago (June 1, 1972)
Published Online	17 years, 5 months ago (March 20, 2008)
Published Print	53 years, 3 months ago (June 1, 1972)

Funders 0

None

BibTeX

@article{Schoenenberger_1972, title={Isolation, Biological and Antigenic Properties of a Specific Toxin Formed in Thermally Altered Mouse Skin}, volume={2}, ISSN={1365-2362}, url={http://dx.doi.org/10.1111/j.1365-2362.1972.tb00584.x}, DOI={10.1111/j.1365-2362.1972.tb00584.x}, number={3}, journal={European Journal of Clinical Investigation}, publisher={Wiley}, author={Schoenenberger, G. A. and Allgöwer, M. and Cueni, L. B. and Eppenberger, U. and Städtler, K. E.}, year={1972}, month=jun, pages={154–162} }

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