DOI: 10.1111/j.1365-2362.1970.tb00591.x. The Inhibitory Effect of Phosphonates on the Formation of Calcium Phosphate Crystals <i>in vitro</i> and on Aortic and Kidney Calcification <i>in vivo</i>

The Inhibitory Effect of Phosphonates on the Formation of Calcium Phosphate Crystals in vitro and on Aortic and Kidney Calcification in vivo

10.1111/j.1365-2362.1970.tb00591.x

Crossref journal-article

Wiley

European Journal of Clinical Investigation (311)

Abstract

Abstract 1. Various phosphonates, which are compounds containing C‐P bonds, have been studied to see whether they are able to inhibit, in a manner similar to that of pyrophosphate and the condensed phosphates, the crystallization of calcium phosphate in vitro and the pathological calcification of the aorta and the kidneys of rats given large amounts of vitamin D3.2. Six of the ten compounds studied markedly increased the minimum product, [Ca] × [P], required to induce the precipitation of calcium phosphate in vitro under physiological conditions of pH, ionic strength and temperature. Inhibition was observed at concentrations as low as 10‐7—106M.3. Most of the diphosphonates, particularly those possessing P‐C‐P bonds, showed some ability to inhibit the calcification of the aortas and kidneys of rats treated with large amounts of vitamin D3. The most effective inhibitors were methylene diphosphonate (MDP), ethane‐1‐hydroxy‐1: I‐diphosphonate (EHDP) and diehloromethylene diphosphonate (Cl2MDP).4. The phosphonates that possess P‐C‐P bonds thus appear to have effects on the deposition of calcium phosphate in vitro and in vivo similar to those of inorganic pyrophosphate and the condensed phosphates, which possess P‐O‐P bonds. These phosphonates differ from the condensed phosphates in that they inhibit kidney calcification as well as aortic calcification and are active by mouth as well as parenterally. The wider spectrum of activity of the phosphonates in vivo may be due to the fact that they are more resistant to chemical and enzymic breakdown.5. Phosphonates might be used therapeutically in man against diseases in which calcium salts deposit in soft tissues.

Bibliography

Fleisch, H. A., Russell, R. G. G., Bisaz, S., MÃ¼hlbauer, R. C., & Williams, D. A. (1970). The Inhibitory Effect of Phosphonates on the Formation of Calcium Phosphate Crystals in vitro and on Aortic and Kidney Calcification in vivo. European Journal of Clinical Investigation, 1(1), 12â18. Portico.

Authors 5

H. A. Fleisch (first)
R. G. G. Russell (additional)
S. Bisaz (additional)
R. C. Mühlbauer (additional)
D. A. Williams (additional)

References 21 Referenced 263

10.1152/ajplegacy.1961.200.6.1296 / Amer. J. Physiol. / Mechanisms of calcification: role of collagen, polyphosphates, and phosphatase by Fleisch H. (1961)
10.1038/212901a0
10.1038/195911a0
Russell R. G. G. Fleisch H.:Inorganic pyrophosphate and pyrophosphatases in calcification and calcium homeostasis.Clin. Orthop.(In press.).
{'key': 'e_1_2_1_6_2', 'first-page': '363', 'article-title': 'The inhibition by condensed phosphates of aortic calcification induced by Vitamin D3 in rats', 'volume': '35', 'author': 'Schibler D.', 'year': '1968', 'journal-title': 'Clin. Sci.'} / Clin. Sci. / The inhibition by condensed phosphates of aortic calcification induced by Vitamin D3 in rats by Schibler D. (1968)
10.1139/y66-024
10.1007/BF01901138
10.3181/00379727-123-31480
10.1042/bj1020053
10.1042/bj1041011
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{'key': 'e_1_2_1_13_2', 'first-page': 'CR174', 'article-title': "L'activité pyrophosphatasique de la phosphatase alcaline", 'volume': '25', 'author': 'Casey P.', 'year': '1967', 'journal-title': 'Helv. physiol. Acta'} / Helv. physiol. Acta / L'activité pyrophosphatasique de la phosphatase alcaline by Casey P. (1967)
10.1042/bj0570595
10.1007/BF02058658
10.1007/BF02065192
10.1126/science.165.3899.1264
10.1016/0003-9969(68)90146-5
10.1126/science.165.3899.1262
10.1038/223211a0
Gasser A. Richelle L. Fleisch H.:The influence of diphosphonates on calcium kinetics. Proc. 7th Europ. Symp. Calc. Tiss. Moutecatini1970.
10.1016/S0140-6736(69)92293-4

Dates

Type	When
Created	17 years, 5 months ago (March 20, 2008, 10:46 a.m.)
Deposited	1 year, 9 months ago (Nov. 10, 2023, 3:24 p.m.)
Indexed	1 day, 14 hours ago (Sept. 4, 2025, 10:27 a.m.)
Issued	55 years, 7 months ago (Feb. 1, 1970)
Published	55 years, 7 months ago (Feb. 1, 1970)
Published Online	17 years, 5 months ago (March 20, 2008)
Published Print	55 years, 7 months ago (Feb. 1, 1970)

Funders 0

None

BibTeX

@article{Fleisch_1970, title={The Inhibitory Effect of Phosphonates on the Formation of Calcium Phosphate Crystals in vitro and on Aortic and Kidney Calcification in vivo}, volume={1}, ISSN={1365-2362}, url={http://dx.doi.org/10.1111/j.1365-2362.1970.tb00591.x}, DOI={10.1111/j.1365-2362.1970.tb00591.x}, number={1}, journal={European Journal of Clinical Investigation}, publisher={Wiley}, author={Fleisch, H. A. and Russell, R. G. G. and Bisaz, S. and Mühlbauer, R. C. and Williams, D. A.}, year={1970}, month=feb, pages={12–18} }

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