DOI: 10.1111/j.1365-2249.1994.tb06544.x. Apparent killing of <i>Mycobacterium tuberculosis</i> by cytokine-activated human monocytes can be an artefact of a cytotoxic effect on the monocytes

Apparent killing of Mycobacterium tuberculosis by cytokine-activated human monocytes can be an artefact of a cytotoxic effect on the monocytes

10.1111/j.1365-2249.1994.tb06544.x

Crossref journal-article

Oxford University Press (OUP)

Clinical and Experimental Immunology (286)

Abstract

SUMMARY A protocol using combined exposure to interferon-gamma (IFN-γ), calcitriol and tumour necrosis factor-alpha (TNF-α) has been reported to activate human monocytes in vitro to kill Mycobacterium tuberculosis. We have attempted to repeat the findings in two laboratories, with negative results: treated cells were no different from untreated cells in this respect. However, the treated cells were more sensitive to a toxic effect of the bacteria. We suggest that the reported dramatic mycobacterial killing may have been an illusory consequence of the toxicity leading to cell lysis and loss of the liberated bacteria from the assay.

Bibliography

WARWICK-DAVIES, J., DHILLON, J., OâBRIEN, L., ANDREW, P. W., & LOWRIE, D. B. (1994). Apparent killing of Mycobacterium tuberculosis by cytokine-activated human monocytes can be an artefact of a cytotoxic effect on the monocytes. Clinical and Experimental Immunology, 96(2), 214â217.

Authors 5

J WARWICK-DAVIES (first)
J DHILLON (additional)
L O'BRIEN (additional)
P W ANDREW (additional)
D B LOWRIE (additional)

References 11 Referenced 25

{'key': '2022011615420422600_b1', 'first-page': '235', 'volume-title': 'The biology of the Mycobacteria', 'author': 'Lowrie', 'year': '1983'} / The biology of the Mycobacteria by Lowrie (1983)
10.1016/0378-1097(92)90273-Q / FEMS Microbiol Immunol / Comparative ability of human monocytes and macrophages to control the intracellular growth of Mycobacterium avium and Mycobacterium tuberculosis. effect of interferon-gamma and indomethacin by Carvalho de Sousa (1992)
10.1128/iai.58.3.632-638.1990 / Infect Immun / Relative permissiveness of macrophages from black and white people for virulent tubercle bacilli by Crowle (1990)
10.1128/iai.50.1.1-8.1985 / Infect Immun / Gamma interferon activates human macrophages to become tumoricidal and Leishmanicidal but enhances replication of macrophage-associated mycobacateria by Douvas (1985)
{'key': '2022011615420422600_b5', 'first-page': '159', 'article-title': 'Vitamin D3, gamma interferon, and control of proliferation of Mycobacterium tuberculosis by human monocytes', 'volume': '57', 'author': 'Rook', 'year': '1986', 'journal-title': 'Immunol'} / Immunol / Vitamin D3, gamma interferon, and control of proliferation of Mycobacterium tuberculosis by human monocytes by Rook (1986)
{'key': '2022011615420422600_b6', 'first-page': '311', 'volume-title': 'Mononuclear phagocytes: physiology and pathology', 'author': 'Andrew', 'year': '1985'} / Mononuclear phagocytes: physiology and pathology by Andrew (1985)
10.1111/j.1365-2249.1991.tb08149.x / Clin Exp Immunol / Killing of Mycobacterium tuberculosis within human monocytes: activation by cytokines and calcitriol by Denis (1991)
10.1016/0003-2697(80)90165-7 / Analyt Biochem / A simple, rapid and sensitive DNA assay procedure by Labarca (1980)
{'key': '2022011615420422600_b9', 'first-page': '419', 'article-title': 'Hydrogen peroxide and superoxide release from alveolar macrophages from normal and BCG vaccinated guinea pigs after intravenous challenge with Mycobacterium tuberculosis', 'volume': '62', 'author': 'Jackett', 'year': '1981', 'journal-title': 'Brit J Exp Pathol'} / Brit J Exp Pathol / Hydrogen peroxide and superoxide release from alveolar macrophages from normal and BCG vaccinated guinea pigs after intravenous challenge with Mycobacterium tuberculosis by Jackett (1981)
10.1128/iai.59.8.2567-2572.1991 / Infect Immun / Effect of mycobacteria on sensitivity to the cytotoxic effects of tumour necrosis factor by Filley (1991)
{'key': '2022011615420422600_b11', 'first-page': '505', 'article-title': 'The effects of Mycobacterium tuberculosis on the susceptibility of human cells to the stimulatory and toxic effects of tumour necrosis factor', 'volume': '77', 'author': 'Filley', 'year': '1992', 'journal-title': 'Immunol.'} / Immunol. / The effects of Mycobacterium tuberculosis on the susceptibility of human cells to the stimulatory and toxic effects of tumour necrosis factor by Filley (1992)

Dates

Type	When
Created	15 years, 1 month ago (July 27, 2010, 5:38 a.m.)
Deposited	3 years, 7 months ago (Jan. 16, 2022, 10:43 a.m.)
Indexed	1 month, 3 weeks ago (July 11, 2025, 6:35 a.m.)
Issued	31 years, 4 months ago (May 1, 1994)
Published	31 years, 4 months ago (May 1, 1994)
Published Online	17 years, 2 months ago (June 28, 2008)
Published Print	17 years, 2 months ago (June 28, 2008)

Funders 0

None

BibTeX

@article{WARWICK_DAVIES_1994, title={Apparent killing of Mycobacterium tuberculosis by cytokine-activated human monocytes can be an artefact of a cytotoxic effect on the monocytes}, volume={96}, ISSN={1365-2249}, url={http://dx.doi.org/10.1111/j.1365-2249.1994.tb06544.x}, DOI={10.1111/j.1365-2249.1994.tb06544.x}, number={2}, journal={Clinical and Experimental Immunology}, publisher={Oxford University Press (OUP)}, author={WARWICK-DAVIES, J and DHILLON, J and O’BRIEN, L and ANDREW, P W and LOWRIE, D B}, year={1994}, month=may, pages={214–217} }

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