DOI: 10.1111/j.1365-2184.1995.tb00062.x. A quantitative method for the analysis of mammalian cell proliferation in culture in terms of dividing and non‐dividing cells

A quantitative method for the analysis of mammalian cell proliferation in culture in terms of dividing and non‐dividing cells

10.1111/j.1365-2184.1995.tb00062.x

Crossref journal-article

Wiley

Cell Proliferation (311)

Abstract

AbstractThe application of the exponential growth equation is the standard method employed in the quantitative analyses of mammalian cell proliferation in culture. This method is based on the implicit assumption that, within a cell population under study, all division events give rise to daughter cells that always divide. When a cell population does not adhere to this assumption, use of the exponential growth equation leads to errors in the determination of both population doubling time and cell generation time. We have derived a more general growth equation that defines cell growth in terms of the dividing fraction of daughter cells. This equation can account for population growth kinetics that derive from the generation of both dividing and non‐dividing cells. As such, it provides a sensitive method for detecting non‐exponential division dynamics. In addition, this equation can be used to determine when it is appropriate to use the standard exponential growth equation for the estimation of doubling time and generation time.

Bibliography

Sherley, J. L., Stadler, P. B., & Stadler, J. S. (1995). A quantitative method for the analysis of mammalian cell proliferation in culture in terms of dividing and nonâdividing cells. Cell Proliferation, 28(3), 137â144. Portico.

Authors 3

J. L. Sherley (first)
P. B. Stadler (additional)
J. Scott Stadler (additional)

References 12 Referenced 68

{'key': 'e_1_2_1_2_1', 'volume-title': 'Nonlinear Parameter Estimation', 'author': 'Bard Y', 'year': '1974'} / Nonlinear Parameter Estimation by Bard Y (1974)
10.1038/255197a0
{'key': 'e_1_2_1_4_1', 'first-page': '128', 'volume-title': 'American Type Culture Collection Catalogue of Cell Lines and Hybridomas', 'author': 'Hay R', 'year': '1992'} / American Type Culture Collection Catalogue of Cell Lines and Hybridomas by Hay R (1992)
10.1016/0014-4827(65)90211-9
10.1093/jnci/56.4.843
10.1016/S0021-9258(18)54302-7
10.1073/pnas.92.1.136
10.1016/S0091-679X(08)62102-0
{'key': 'e_1_2_1_10_1', 'first-page': '193', 'article-title': 'Cell loss from experimental tumours', 'volume': '1', 'author': 'Steel GG', 'year': '1968', 'journal-title': 'Cell Tissue Kinet.'} / Cell Tissue Kinet. / Cell loss from experimental tumours by Steel GG (1968)
10.1016/S0091-679X(08)62144-5
10.1111/j.1365-2184.1991.tb01155.x
{'key': 'e_1_2_1_13_1', 'first-page': '561', 'article-title': 'New methods for calculating kinetic properties of cells in vitro using pulse labelling with bromodeoxyuridine', 'volume': '23', 'author': 'White RA', 'year': '1990', 'journal-title': 'Cell Tissue Kinet.'} / Cell Tissue Kinet. / New methods for calculating kinetic properties of cells in vitro using pulse labelling with bromodeoxyuridine by White RA (1990)

Dates

Type	When
Created	17 years, 4 months ago (May 1, 2008, 5:17 p.m.)
Deposited	1 year, 10 months ago (Oct. 26, 2023, 7:06 p.m.)
Indexed	1 month ago (Aug. 5, 2025, 9:10 a.m.)
Issued	30 years, 6 months ago (March 1, 1995)
Published	30 years, 6 months ago (March 1, 1995)
Published Online	17 years, 2 months ago (June 28, 2008)
Published Print	30 years, 6 months ago (March 1, 1995)

Funders 0

None

BibTeX

@article{Sherley_1995, title={A quantitative method for the analysis of mammalian cell proliferation in culture in terms of dividing and non‐dividing cells}, volume={28}, ISSN={1365-2184}, url={http://dx.doi.org/10.1111/j.1365-2184.1995.tb00062.x}, DOI={10.1111/j.1365-2184.1995.tb00062.x}, number={3}, journal={Cell Proliferation}, publisher={Wiley}, author={Sherley, J. L. and Stadler, P. B. and Stadler, J. Scott}, year={1995}, month=mar, pages={137–144} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        5
      ]
    ],
    "date-time": "2025-08-05T13:10:31Z",
    "timestamp": 1754399431471
  },
  "reference-count": 12,
  "publisher": "Wiley",
  "issue": "3",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2008,
            6,
            28
          ]
        ],
        "date-time": "2008-06-28T00:00:00Z",
        "timestamp": 1214611200000
      },
      "content-version": "vor",
      "delay-in-days": 4868,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1995,
        3
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title><jats:p>The application of the exponential growth equation is the standard method employed in the quantitative analyses of mammalian cell proliferation in culture. This method is based on the implicit assumption that, within a cell population under study, all division events give rise to daughter cells that always divide. When a cell population does not adhere to this assumption, use of the exponential growth equation leads to errors in the determination of both population doubling time and cell generation time. We have derived a more general growth equation that defines cell growth in terms of the dividing fraction of daughter cells. This equation can account for population growth kinetics that derive from the generation of both dividing and non\u2010dividing cells. As such, it provides a sensitive method for detecting non\u2010exponential division dynamics. In addition, this equation can be used to determine when it is appropriate to use the standard exponential growth equation for the estimation of doubling time and generation time.</jats:p>",
  "DOI": "10.1111/j.1365-2184.1995.tb00062.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2008,
        5,
        1
      ]
    ],
    "date-time": "2008-05-01T21:17:40Z",
    "timestamp": 1209676660000
  },
  "page": "137-144",
  "source": "Crossref",
  "is-referenced-by-count": 68,
  "title": "A quantitative method for the analysis of mammalian cell proliferation in culture in terms of dividing and non\u2010dividing cells",
  "prefix": "10.1111",
  "volume": "28",
  "author": [
    {
      "given": "J. L.",
      "family": "Sherley",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "P. B.",
      "family": "Stadler",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "J. Scott",
      "family": "Stadler",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2008,
        6,
        28
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_1",
      "volume-title": "Nonlinear Parameter Estimation",
      "author": "Bard Y",
      "year": "1974"
    },
    {
      "key": "e_1_2_1_3_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/255197a0"
    },
    {
      "key": "e_1_2_1_4_1",
      "first-page": "128",
      "volume-title": "American Type Culture Collection Catalogue of Cell Lines and Hybridomas",
      "author": "Hay R",
      "year": "1992"
    },
    {
      "key": "e_1_2_1_5_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-4827(65)90211-9"
    },
    {
      "key": "e_1_2_1_6_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/jnci/56.4.843"
    },
    {
      "key": "e_1_2_1_7_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)54302-7"
    },
    {
      "key": "e_1_2_1_8_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.92.1.136"
    },
    {
      "key": "e_1_2_1_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0091-679X(08)62102-0"
    },
    {
      "key": "e_1_2_1_10_1",
      "first-page": "193",
      "article-title": "Cell loss from experimental tumours",
      "volume": "1",
      "author": "Steel GG",
      "year": "1968",
      "journal-title": "Cell Tissue Kinet."
    },
    {
      "key": "e_1_2_1_11_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0091-679X(08)62144-5"
    },
    {
      "key": "e_1_2_1_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2184.1991.tb01155.x"
    },
    {
      "key": "e_1_2_1_13_1",
      "first-page": "561",
      "article-title": "New methods for calculating kinetic properties of cells in vitro using pulse labelling with bromodeoxyuridine",
      "volume": "23",
      "author": "White RA",
      "year": "1990",
      "journal-title": "Cell Tissue Kinet."
    }
  ],
  "container-title": "Cell Proliferation",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2184.1995.tb00062.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2184.1995.tb00062.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        10,
        26
      ]
    ],
    "date-time": "2023-10-26T23:06:46Z",
    "timestamp": 1698361606000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1111/j.1365-2184.1995.tb00062.x"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1995,
        3
      ]
    ]
  },
  "references-count": 12,
  "journal-issue": {
    "issue": "3",
    "published-print": {
      "date-parts": [
        [
          1995,
          3
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1111/j.1365-2184.1995.tb00062.x"
  ],
  "URL": "http://dx.doi.org/10.1111/j.1365-2184.1995.tb00062.x",
  "relation": {},
  "ISSN": [
    "0960-7722",
    "1365-2184"
  ],
  "subject": [],
  "container-title-short": "Cell Proliferation",
  "published": {
    "date-parts": [
      [
        1995,
        3
      ]
    ]
  }
}