DOI: 10.1111/j.1365-2141.1995.tb05277.x. Increased expression of Fas antigen on bone marrow CD34<sup>+</sup> cells of patients with aplastic anaemia

Increased expression of Fas antigen on bone marrow CD34+ cells of patients with aplastic anaemia

10.1111/j.1365-2141.1995.tb05277.x

Crossref journal-article

Wiley

British Journal of Haematology (311)

Abstract

Summary Fas antigen, a receptor molecule that mediates signals for programmed cell death, is involved in T‐cell‐mediated killing of malignant, virus‐infected or allogeneic target cells. Interferon‐γ (IFN‐γ) and tumour necrosis factored (TNF‐α), potent inhibitors of haemopoiesis, enhance Fas receptor expression on bone marrow (BM) CD34+ cells, and both cytokines render haemopoietic progenitor cells susceptible to Fas‐mediated inhibition of colony formation due to the induction of apoptosis. Haemopoietic suppression in aplastic anaemia (AA) has been associated with aberrant IFN‐γ, increased TNF‐β expression, and elevated numbers of activated cytotoxic T‐cells in marrow. We have now examined Fas antigen expression in fresh AA BM samples. In normal individuals few CD34+ cells expressed Fas antigen and normal marrow cells had low sensitivity to Fas‐mediated inhibition of colony formation. In contrast, in early AA, BM CD34+ cells showed markedly increased percentages of Fas receptor‐expressing CD34+ cells, which correlated with increased sensitivity of AA marrow cells to anti‐Fas antibody‐mediated inhibition of colony formation. The proportion of Fas antigen‐bearing cells was lower in recovered patients’BM. Fas antigen was also detected in the marrow of some patients with myelodysplasia, especially the hypocellular variant. These results are consistent with the hypothesis that AA CD34+ cells, probably including haemopoietic progenitor cells, express high levels of Fas receptor due to in vivo exposure to IFN‐γ and/or TNF‐α and are suitable targets for T‐cell‐mediated killing. Our results suggest that the Fas receptor/Fas ligand system are involved in the pathophysiology of BM failure.

Bibliography

Maciejewski, J. P., Selleri, C., Sato, T., Anderson, S., & Young, N. S. (1995). Increased expression of Fas antigen on bone marrow CD34+ cells of patients with aplastic anaemia. British Journal of Haematology, 91(1), 245â252. Portico.

Authors 5

Jaroslaw P. Maciejewski (first)
Carmine Selleri (additional)
Tadatsugu Sato (additional)
Stacie Anderson (additional)
Neal S. Young (additional)

References 54 Referenced 213

10.1182/blood.V85.5.1348.bloodjournal8551348 / Blood / Antithymocyte globulin, cyclosporin, and granulocyte colony‐stimulating factor in patients with aquired severe aplastic anemia (SAA): a pilot study of the EBMT SAA working party by Bacigalupo A. (1985)
10.1055/s-2008-1041072
10.1111/j.1365-2141.1982.tb08475.x / British Journal of Haematology / Proposals for the classification of the myelodysplastic syndromes by Bennett J.M. (1982)
10.7326/0003-4819-109-9-695
10.1182/blood.V48.1.63.63 / Blood / Severe aplastic anemia: a prospective study of the effect of early transplantation on acute mortality by Camitta B.N. (1976)
10.1146/annurev.iy.08.040190.003243
10.1126/science.7510905
{'key': 'e_1_2_1_9_1', 'first-page': '10', 'article-title': 'Antigenic analysis of hematopoiesis. VI. Characterization of my‐10‐positive progenitor cells in normal human bone marrow', 'volume': '15', 'author': 'Civin C.I.', 'year': '1987', 'journal-title': 'Experimental Hematology'} / Experimental Hematology / Antigenic analysis of hematopoiesis. VI. Characterization of my‐10‐positive progenitor cells in normal human bone marrow by Civin C.I. (1987)
10.4049/jimmunol.133.1.157 / Journal of Immunology / Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cell surface antigen defined by monoclonal antibody raised against KG‐la cells by Civin C.J. (1984)
10.1182/blood.V81.11.2972.2972
10.1182/blood.V83.10.3101a.3101a / Blood / High expression of APO‐1 (CD95) on T lymphocytes from human immunodeficiency virus‐1‐infected children. (Letter) by Debatin K.M. (1994)
10.1073/pnas.91.11.4930
{'key': 'e_1_2_1_14_1', 'first-page': '127', 'volume-title': 'Molecular Biology of Haematopoksis', 'author': 'Hatake K.', 'year': '1994'} / Molecular Biology of Haematopoksis by Hatake K. (1994)
10.1001/jama.1992.03480150057037 / Journal of the American Medical Association / Hepatitis/ aplasia syndrome: non A, non B, non C by Hibbs J. (1992)
10.1002/hep.1840190606
10.1056/NEJM197701062960103
10.1016/0092-8674(91)90614-5
10.1126/science.7518614
{'key': 'e_1_2_1_20_1', 'first-page': '345', 'article-title': 'Increased expression of TNF‐beta in patients with aplastic anemia. (Abstract)', 'volume': '82', 'author': 'Katevas P.', 'year': '1993', 'journal-title': 'Blood'} / Blood / Increased expression of TNF‐beta in patients with aplastic anemia. (Abstract) by Katevas P. (1993)
10.1016/0145-2126(94)90034-5
{'key': 'e_1_2_1_22_1', 'first-page': '1102', 'article-title': 'Bone marrow and peripheral blood lymphocyte phenotype in patients with bone marrow failure', 'volume': '22', 'author': 'Maciejewski J.P.', 'year': '1994', 'journal-title': 'Experimental Hematology'} / Experimental Hematology / Bone marrow and peripheral blood lymphocyte phenotype in patients with bone marrow failure by Maciejewski J.P. (1994)
{'key': 'e_1_2_1_23_1', 'first-page': '1112', 'article-title': 'Expression of a stem cell inhibitor (SCI/LD78) gene in patients with bone marrow failure', 'volume': '20', 'author': 'Maciejewski J.P.', 'year': '1992', 'journal-title': 'Experimental Hematology'} / Experimental Hematology / Expression of a stem cell inhibitor (SCI/LD78) gene in patients with bone marrow failure by Maciejewski J.P. (1992)
10.1182/blood.V85.11.3183.bloodjournal85113183 / Blood / Fas antigen expression on CD34+ human marrow cells is induced by interferon‐γ and tumor necrosis factor‐α and potentiates cytokine‐mediated hematopoietic suppression in vitro by Maciejewski J.P. (1995)
{'key': 'e_1_2_1_25_1', 'first-page': '269', 'article-title': 'Autoimmune myelopathies', 'volume': '28', 'author': 'Marmont A.M.', 'year': '1991', 'journal-title': 'Seminars in Hematology'} / Seminars in Hematology / Autoimmune myelopathies by Marmont A.M. (1991)
{'key': 'e_1_2_1_26_1', 'first-page': '322', 'article-title': 'Autoimmune myelodysplasias', 'volume': '28', 'author': 'Miesher P.A.', 'year': '1991', 'journal-title': 'Seminars in Hematology'} / Seminars in Hematology / Autoimmune myelodysplasias by Miesher P.A. (1991)
10.1182/blood.V79.10.2532.bloodjournal79102532 / Blood / Interferon gamma gene expression in unstimulated bone marrow mononuclear cells predicts a response to cyclosporine therapy in aplastic anemia by Nakao S. (1992)
10.1111/j.1365-2141.1980.tb07143.x
10.7326/0003-4819-120-6-199403150-00003
10.1016/S0021-9258(19)50076-X / Journal of Biological Chemistry / Purification and molecular cloning of the Apo‐1 cell surface antigen, a member of the tumor necrosis factor/nerve growth factor receptor superfamily: sequence identity with the Fas antigen by Oehm A. (1992)
{'key': 'e_1_2_1_31_1', 'first-page': '255', 'article-title': 'Selective targeting of the immune response in autoimmune demyelination', 'volume': '161', 'author': 'Oksenburg J.R.', 'year': '1994', 'journal-title': 'Neurology'} / Neurology / Selective targeting of the immune response in autoimmune demyelination by Oksenburg J.R. (1994)
10.1097/00002371-199310000-00011
10.7326/0003-4819-113-6-435
10.1016/0268-960X(91)90037-D
10.1182/blood.V85.11.3058.bloodjournal85113058 / Blood / Improved response rate of severe aplastic anemia to intensive immunosuppression with antithymocyte globulin and cyclosporin by Rosenfeld S. (1995)
10.1016/S0950-3536(89)80010-1
10.1084/jem.177.1.195
{'key': 'e_1_2_1_38_1', 'first-page': '749', 'article-title': 'Haemopoietic progenitor cells are reduced in aplastic anaemia', 'volume': '84', 'author': 'Scopes J.', 'year': '1994', 'journal-title': 'British Journal of Haematology'} / British Journal of Haematology / Haemopoietic progenitor cells are reduced in aplastic anaemia by Scopes J. (1994)
Selleri C. Sato T. Anderson S. Young N.S.&Maciejewski J.P.(1995)Interferon‐γ and tumor necrosis factor‐α suppress both early and late stages of hematopoiesis and induce programmed cell death.Journal of Cellular Physiology in press. (10.1002/jcp.1041650312)
10.1182/blood.V54.1.180.180 / Blood / Coculture studies of sixteen untransfused patients with aplastic anemia by Singer J.W. (1979)
10.1084/jem.180.3.1177
10.1182/blood.V60.3.758.758 / Blood / Cells with Fc‐γ from normal donors suppress granulocytic macrophage colony formation by Spitzer G. (1982)
{'issue': '1', 'key': 'e_1_2_1_43_1', 'first-page': '229a', 'article-title': 'Antithymocyte globulin for hypoplastic myelodysplastic syndrome', 'volume': '72', 'author': 'Sullecki M.', 'year': '1988', 'journal-title': 'Blood'} / Blood / Antithymocyte globulin for hypoplastic myelodysplastic syndrome by Sullecki M. (1988)
10.1016/0092-8674(93)90326-L
10.1099/0022-1317-74-11-2347
10.1182/blood.V55.2.211.211 / Blood / In vitro tests for distinguishing possible immune‐mediated aplastic anemia from transfusion‐induced sensitization by Torok‐Storb B.J. (1980)
{'key': 'e_1_2_1_47_1', 'first-page': '1268', 'article-title': 'Measurement of serum cytokine levels in patients with myelodysplastic syndromes', 'volume': '6', 'author': 'Verhoef G.E.', 'year': '1992', 'journal-title': 'Leukemia'} / Leukemia / Measurement of serum cytokine levels in patients with myelodysplastic syndromes by Verhoef G.E. (1992)
10.1182/blood.V78.5.1268.1268 / Blood / Analysis at the clonal level of T cell phenotype and functions in severe aplastic anemia patients by Viale M. (1991)
10.4049/jimmunol.148.4.1274 / Journal of Immunology / The cDNA structure, expression, and chromosomal assignment of the mouse Fas antigen by Watanabe‐Fukunaga R. (1992)
10.1182/blood.V79.6.1385.1385 / Blood / The problem of clonality in aplastic anemia. Dr Dameshek's riddle, restated by Young N.S. (1992)
{'volume-title': 'Aplastic Anemia, Acquired and Inherited', 'year': '1994', 'author': 'Young N.S.', 'key': 'e_1_2_1_51_1'} / Aplastic Anemia, Acquired and Inherited by Young N.S. (1994)
10.1182/blood.V85.12.3367.bloodjournal85123367 / Blood / The treatment of severe aplastic anemia by Young N.S. (1995)
10.1182/blood.V72.6.1861.1861 / Blood / A multicenter trial of antithymocyte globulin in bone marrow failure by Young N.S. (1988)
10.4049/jimmunol.133.2.769 / Journal of Immunology / Interferon is the suppressor of hematopoiesis generated by stimulated lymphocytes in vitro by Zoumbos N. (1984)
10.1056/NEJM198501313120501

Dates

Type	When
Created	17 years, 5 months ago (March 12, 2008, 7:22 a.m.)
Deposited	1 year, 10 months ago (Oct. 25, 2023, 2:23 p.m.)
Indexed	1 week ago (Aug. 28, 2025, 8:39 a.m.)
Issued	30 years ago (Sept. 1, 1995)
Published	30 years ago (Sept. 1, 1995)
Published Online	17 years, 5 months ago (March 12, 2008)
Published Print	30 years ago (Sept. 1, 1995)

Funders 0

None

BibTeX

@article{Maciejewski_1995, title={Increased expression of Fas antigen on bone marrow CD34+ cells of patients with aplastic anaemia}, volume={91}, ISSN={1365-2141}, url={http://dx.doi.org/10.1111/j.1365-2141.1995.tb05277.x}, DOI={10.1111/j.1365-2141.1995.tb05277.x}, number={1}, journal={British Journal of Haematology}, publisher={Wiley}, author={Maciejewski, Jaroslaw P. and Selleri, Carmine and Sato, Tadatsugu and Anderson, Stacie and Young, Neal S.}, year={1995}, month=sep, pages={245–252} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        28
      ]
    ],
    "date-time": "2025-08-28T12:39:54Z",
    "timestamp": 1756384794608
  },
  "reference-count": 54,
  "publisher": "Wiley",
  "issue": "1",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2008,
            3,
            12
          ]
        ],
        "date-time": "2008-03-12T00:00:00Z",
        "timestamp": 1205280000000
      },
      "content-version": "vor",
      "delay-in-days": 4576,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [
      "onlinelibrary.wiley.com"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1995,
        9
      ]
    ]
  },
  "abstract": "<jats:p><jats:bold>Summary</jats:bold> Fas antigen, a receptor molecule that mediates signals for programmed cell death, is involved in T\u2010cell\u2010mediated killing of malignant, virus\u2010infected or allogeneic target cells. Interferon\u2010<jats:italic>\u03b3</jats:italic> (IFN\u2010<jats:italic>\u03b3</jats:italic>) and tumour necrosis factored (TNF\u2010<jats:italic>\u03b1</jats:italic>), potent inhibitors of haemopoiesis, enhance Fas receptor expression on bone marrow (BM) CD34<jats:sup>+</jats:sup> cells, and both cytokines render haemopoietic progenitor cells susceptible to Fas\u2010mediated inhibition of colony formation due to the induction of apoptosis. Haemopoietic suppression in aplastic anaemia (AA) has been associated with aberrant IFN\u2010<jats:italic>\u03b3</jats:italic>, increased TNF\u2010<jats:italic>\u03b2</jats:italic> expression, and elevated numbers of activated cytotoxic T\u2010cells in marrow. We have now examined Fas antigen expression in fresh AA BM samples. In normal individuals few CD34<jats:sup>+</jats:sup> cells expressed Fas antigen and normal marrow cells had low sensitivity to Fas\u2010mediated inhibition of colony formation. In contrast, in early AA, BM CD34<jats:sup>+</jats:sup> cells showed markedly increased percentages of Fas receptor\u2010expressing CD34<jats:sup>+</jats:sup> cells, which correlated with increased sensitivity of AA marrow cells to anti\u2010Fas antibody\u2010mediated inhibition of colony formation. The proportion of Fas antigen\u2010bearing cells was lower in recovered patients\u2019BM. Fas antigen was also detected in the marrow of some patients with myelodysplasia, especially the hypocellular variant. These results are consistent with the hypothesis that AA CD34<jats:sup>+</jats:sup> cells, probably including haemopoietic progenitor cells, express high levels of Fas receptor due to <jats:italic>in vivo</jats:italic> exposure to IFN\u2010<jats:italic>\u03b3</jats:italic> and/or TNF\u2010\u03b1 and are suitable targets for T\u2010cell\u2010mediated killing. Our results suggest that the Fas receptor/Fas ligand system are involved in the pathophysiology of BM failure.</jats:p>",
  "DOI": "10.1111/j.1365-2141.1995.tb05277.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2008,
        3,
        12
      ]
    ],
    "date-time": "2008-03-12T11:22:20Z",
    "timestamp": 1205320940000
  },
  "page": "245-252",
  "update-policy": "http://dx.doi.org/10.1002/crossmark_policy",
  "source": "Crossref",
  "is-referenced-by-count": 213,
  "title": "Increased expression of Fas antigen on bone marrow CD34<sup>+</sup> cells of patients with aplastic anaemia",
  "prefix": "10.1111",
  "volume": "91",
  "author": [
    {
      "given": "Jaroslaw P.",
      "family": "Maciejewski",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Carmine",
      "family": "Selleri",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Tadatsugu",
      "family": "Sato",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Stacie",
      "family": "Anderson",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Neal S.",
      "family": "Young",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2008,
        3,
        12
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_1",
      "doi-asserted-by": "crossref",
      "first-page": "1348",
      "DOI": "10.1182/blood.V85.5.1348.bloodjournal8551348",
      "article-title": "Antithymocyte globulin, cyclosporin, and granulocyte colony\u2010stimulating factor in patients with aquired severe aplastic anemia (SAA): a pilot study of the EBMT SAA working party",
      "volume": "85",
      "author": "Bacigalupo A.",
      "year": "1985",
      "journal-title": "Blood"
    },
    {
      "key": "e_1_2_1_3_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1055/s-2008-1041072"
    },
    {
      "key": "e_1_2_1_4_1",
      "doi-asserted-by": "crossref",
      "first-page": "189",
      "DOI": "10.1111/j.1365-2141.1982.tb08475.x",
      "article-title": "Proposals for the classification of the myelodysplastic syndromes",
      "volume": "33",
      "author": "Bennett J.M.",
      "year": "1982",
      "journal-title": "British Journal of Haematology"
    },
    {
      "key": "e_1_2_1_5_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.7326/0003-4819-109-9-695"
    },
    {
      "key": "e_1_2_1_6_1",
      "doi-asserted-by": "crossref",
      "first-page": "63",
      "DOI": "10.1182/blood.V48.1.63.63",
      "article-title": "Severe aplastic anemia: a prospective study of the effect of early transplantation on acute mortality",
      "volume": "48",
      "author": "Camitta B.N.",
      "year": "1976",
      "journal-title": "Blood"
    },
    {
      "key": "e_1_2_1_7_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.iy.08.040190.003243"
    },
    {
      "key": "e_1_2_1_8_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.7510905"
    },
    {
      "key": "e_1_2_1_9_1",
      "first-page": "10",
      "article-title": "Antigenic analysis of hematopoiesis. VI. Characterization of my\u201010\u2010positive progenitor cells in normal human bone marrow",
      "volume": "15",
      "author": "Civin C.I.",
      "year": "1987",
      "journal-title": "Experimental Hematology"
    },
    {
      "key": "e_1_2_1_10_1",
      "doi-asserted-by": "crossref",
      "first-page": "157",
      "DOI": "10.4049/jimmunol.133.1.157",
      "article-title": "Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cell surface antigen defined by monoclonal antibody raised against KG\u2010la cells",
      "volume": "133",
      "author": "Civin C.J.",
      "year": "1984",
      "journal-title": "Journal of Immunology"
    },
    {
      "key": "e_1_2_1_11_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1182/blood.V81.11.2972.2972"
    },
    {
      "key": "e_1_2_1_12_1",
      "doi-asserted-by": "crossref",
      "first-page": "3101",
      "DOI": "10.1182/blood.V83.10.3101a.3101a",
      "article-title": "High expression of APO\u20101 (CD95) on T lymphocytes from human immunodeficiency virus\u20101\u2010infected children. (Letter)",
      "volume": "83",
      "author": "Debatin K.M.",
      "year": "1994",
      "journal-title": "Blood"
    },
    {
      "key": "e_1_2_1_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.91.11.4930"
    },
    {
      "key": "e_1_2_1_14_1",
      "first-page": "127",
      "volume-title": "Molecular Biology of Haematopoksis",
      "author": "Hatake K.",
      "year": "1994"
    },
    {
      "key": "e_1_2_1_15_1",
      "doi-asserted-by": "crossref",
      "first-page": "2051",
      "DOI": "10.1001/jama.1992.03480150057037",
      "article-title": "Hepatitis/ aplasia syndrome: non A, non B, non C",
      "volume": "267",
      "author": "Hibbs J.",
      "year": "1992",
      "journal-title": "Journal of the American Medical Association"
    },
    {
      "key": "e_1_2_1_16_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/hep.1840190606"
    },
    {
      "key": "e_1_2_1_17_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1056/NEJM197701062960103"
    },
    {
      "key": "e_1_2_1_18_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(91)90614-5"
    },
    {
      "key": "e_1_2_1_19_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.7518614"
    },
    {
      "key": "e_1_2_1_20_1",
      "first-page": "345",
      "article-title": "Increased expression of TNF\u2010beta in patients with aplastic anemia. (Abstract)",
      "volume": "82",
      "author": "Katevas P.",
      "year": "1993",
      "journal-title": "Blood"
    },
    {
      "key": "e_1_2_1_21_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0145-2126(94)90034-5"
    },
    {
      "key": "e_1_2_1_22_1",
      "first-page": "1102",
      "article-title": "Bone marrow and peripheral blood lymphocyte phenotype in patients with bone marrow failure",
      "volume": "22",
      "author": "Maciejewski J.P.",
      "year": "1994",
      "journal-title": "Experimental Hematology"
    },
    {
      "key": "e_1_2_1_23_1",
      "first-page": "1112",
      "article-title": "Expression of a stem cell inhibitor (SCI/LD78) gene in patients with bone marrow failure",
      "volume": "20",
      "author": "Maciejewski J.P.",
      "year": "1992",
      "journal-title": "Experimental Hematology"
    },
    {
      "key": "e_1_2_1_24_1",
      "doi-asserted-by": "crossref",
      "first-page": "3183",
      "DOI": "10.1182/blood.V85.11.3183.bloodjournal85113183",
      "article-title": "Fas antigen expression on CD34+ human marrow cells is induced by interferon\u2010\u03b3 and tumor necrosis factor\u2010\u03b1 and potentiates cytokine\u2010mediated hematopoietic suppression in vitro",
      "volume": "85",
      "author": "Maciejewski J.P.",
      "year": "1995",
      "journal-title": "Blood"
    },
    {
      "key": "e_1_2_1_25_1",
      "first-page": "269",
      "article-title": "Autoimmune myelopathies",
      "volume": "28",
      "author": "Marmont A.M.",
      "year": "1991",
      "journal-title": "Seminars in Hematology"
    },
    {
      "key": "e_1_2_1_26_1",
      "first-page": "322",
      "article-title": "Autoimmune myelodysplasias",
      "volume": "28",
      "author": "Miesher P.A.",
      "year": "1991",
      "journal-title": "Seminars in Hematology"
    },
    {
      "key": "e_1_2_1_27_1",
      "doi-asserted-by": "crossref",
      "first-page": "2532",
      "DOI": "10.1182/blood.V79.10.2532.bloodjournal79102532",
      "article-title": "Interferon gamma gene expression in unstimulated bone marrow mononuclear cells predicts a response to cyclosporine therapy in aplastic anemia",
      "volume": "79",
      "author": "Nakao S.",
      "year": "1992",
      "journal-title": "Blood"
    },
    {
      "key": "e_1_2_1_28_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2141.1980.tb07143.x"
    },
    {
      "key": "e_1_2_1_29_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.7326/0003-4819-120-6-199403150-00003"
    },
    {
      "key": "e_1_2_1_30_1",
      "doi-asserted-by": "crossref",
      "first-page": "10709",
      "DOI": "10.1016/S0021-9258(19)50076-X",
      "article-title": "Purification and molecular cloning of the Apo\u20101 cell surface antigen, a member of the tumor necrosis factor/nerve growth factor receptor superfamily: sequence identity with the Fas antigen",
      "volume": "267",
      "author": "Oehm A.",
      "year": "1992",
      "journal-title": "Journal of Biological Chemistry"
    },
    {
      "key": "e_1_2_1_31_1",
      "first-page": "255",
      "article-title": "Selective targeting of the immune response in autoimmune demyelination",
      "volume": "161",
      "author": "Oksenburg J.R.",
      "year": "1994",
      "journal-title": "Neurology"
    },
    {
      "key": "e_1_2_1_32_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1097/00002371-199310000-00011"
    },
    {
      "key": "e_1_2_1_33_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.7326/0003-4819-113-6-435"
    },
    {
      "key": "e_1_2_1_34_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0268-960X(91)90037-D"
    },
    {
      "key": "e_1_2_1_35_1",
      "doi-asserted-by": "crossref",
      "first-page": "3058",
      "DOI": "10.1182/blood.V85.11.3058.bloodjournal85113058",
      "article-title": "Improved response rate of severe aplastic anemia to intensive immunosuppression with antithymocyte globulin and cyclosporin",
      "volume": "85",
      "author": "Rosenfeld S.",
      "year": "1995",
      "journal-title": "Blood"
    },
    {
      "key": "e_1_2_1_36_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0950-3536(89)80010-1"
    },
    {
      "key": "e_1_2_1_37_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1084/jem.177.1.195"
    },
    {
      "key": "e_1_2_1_38_1",
      "first-page": "749",
      "article-title": "Haemopoietic progenitor cells are reduced in aplastic anaemia",
      "volume": "84",
      "author": "Scopes J.",
      "year": "1994",
      "journal-title": "British Journal of Haematology"
    },
    {
      "key": "e_1_2_1_39_1",
      "doi-asserted-by": "crossref",
      "unstructured": "Selleri C. Sato T. Anderson S. Young N.S.&Maciejewski J.P.(1995)Interferon\u2010\u03b3 and tumor necrosis factor\u2010\u03b1 suppress both early and late stages of hematopoiesis and induce programmed cell death.Journal of Cellular Physiology in press.",
      "DOI": "10.1002/jcp.1041650312"
    },
    {
      "key": "e_1_2_1_40_1",
      "doi-asserted-by": "crossref",
      "first-page": "180",
      "DOI": "10.1182/blood.V54.1.180.180",
      "article-title": "Coculture studies of sixteen untransfused patients with aplastic anemia",
      "volume": "54",
      "author": "Singer J.W.",
      "year": "1979",
      "journal-title": "Blood"
    },
    {
      "key": "e_1_2_1_41_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1084/jem.180.3.1177"
    },
    {
      "key": "e_1_2_1_42_1",
      "doi-asserted-by": "crossref",
      "first-page": "758",
      "DOI": "10.1182/blood.V60.3.758.758",
      "article-title": "Cells with Fc\u2010\u03b3 from normal donors suppress granulocytic macrophage colony formation",
      "volume": "60",
      "author": "Spitzer G.",
      "year": "1982",
      "journal-title": "Blood"
    },
    {
      "issue": "1",
      "key": "e_1_2_1_43_1",
      "first-page": "229a",
      "article-title": "Antithymocyte globulin for hypoplastic myelodysplastic syndrome",
      "volume": "72",
      "author": "Sullecki M.",
      "year": "1988",
      "journal-title": "Blood"
    },
    {
      "key": "e_1_2_1_44_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(93)90326-L"
    },
    {
      "key": "e_1_2_1_45_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1099/0022-1317-74-11-2347"
    },
    {
      "key": "e_1_2_1_46_1",
      "doi-asserted-by": "crossref",
      "first-page": "211",
      "DOI": "10.1182/blood.V55.2.211.211",
      "article-title": "In vitro tests for distinguishing possible immune\u2010mediated aplastic anemia from transfusion\u2010induced sensitization",
      "volume": "55",
      "author": "Torok\u2010Storb B.J.",
      "year": "1980",
      "journal-title": "Blood"
    },
    {
      "key": "e_1_2_1_47_1",
      "first-page": "1268",
      "article-title": "Measurement of serum cytokine levels in patients with myelodysplastic syndromes",
      "volume": "6",
      "author": "Verhoef G.E.",
      "year": "1992",
      "journal-title": "Leukemia"
    },
    {
      "key": "e_1_2_1_48_1",
      "doi-asserted-by": "crossref",
      "first-page": "1268",
      "DOI": "10.1182/blood.V78.5.1268.1268",
      "article-title": "Analysis at the clonal level of T cell phenotype and functions in severe aplastic anemia patients",
      "volume": "78",
      "author": "Viale M.",
      "year": "1991",
      "journal-title": "Blood"
    },
    {
      "key": "e_1_2_1_49_1",
      "doi-asserted-by": "crossref",
      "first-page": "1274",
      "DOI": "10.4049/jimmunol.148.4.1274",
      "article-title": "The cDNA structure, expression, and chromosomal assignment of the mouse Fas antigen",
      "volume": "148",
      "author": "Watanabe\u2010Fukunaga R.",
      "year": "1992",
      "journal-title": "Journal of Immunology"
    },
    {
      "key": "e_1_2_1_50_1",
      "doi-asserted-by": "crossref",
      "first-page": "1385",
      "DOI": "10.1182/blood.V79.6.1385.1385",
      "article-title": "The problem of clonality in aplastic anemia. Dr Dameshek's riddle, restated",
      "volume": "79",
      "author": "Young N.S.",
      "year": "1992",
      "journal-title": "Blood"
    },
    {
      "volume-title": "Aplastic Anemia, Acquired and Inherited",
      "year": "1994",
      "author": "Young N.S.",
      "key": "e_1_2_1_51_1"
    },
    {
      "key": "e_1_2_1_52_1",
      "doi-asserted-by": "crossref",
      "first-page": "3367",
      "DOI": "10.1182/blood.V85.12.3367.bloodjournal85123367",
      "article-title": "The treatment of severe aplastic anemia",
      "volume": "85",
      "author": "Young N.S.",
      "year": "1995",
      "journal-title": "Blood"
    },
    {
      "key": "e_1_2_1_53_1",
      "doi-asserted-by": "crossref",
      "first-page": "1861",
      "DOI": "10.1182/blood.V72.6.1861.1861",
      "article-title": "A multicenter trial of antithymocyte globulin in bone marrow failure",
      "volume": "72",
      "author": "Young N.S.",
      "year": "1988",
      "journal-title": "Blood"
    },
    {
      "key": "e_1_2_1_54_1",
      "doi-asserted-by": "crossref",
      "first-page": "769",
      "DOI": "10.4049/jimmunol.133.2.769",
      "article-title": "Interferon is the suppressor of hematopoiesis generated by stimulated lymphocytes in vitro",
      "volume": "133",
      "author": "Zoumbos N.",
      "year": "1984",
      "journal-title": "Journal of Immunology"
    },
    {
      "key": "e_1_2_1_55_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1056/NEJM198501313120501"
    }
  ],
  "container-title": "British Journal of Haematology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2141.1995.tb05277.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2141.1995.tb05277.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        10,
        25
      ]
    ],
    "date-time": "2023-10-25T18:23:52Z",
    "timestamp": 1698258232000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1111/j.1365-2141.1995.tb05277.x"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1995,
        9
      ]
    ]
  },
  "references-count": 54,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          1995,
          9
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1111/j.1365-2141.1995.tb05277.x"
  ],
  "URL": "http://dx.doi.org/10.1111/j.1365-2141.1995.tb05277.x",
  "relation": {},
  "ISSN": [
    "0007-1048",
    "1365-2141"
  ],
  "subject": [],
  "container-title-short": "Br J Haematol",
  "published": {
    "date-parts": [
      [
        1995,
        9
      ]
    ]
  },
  "assertion": [
    {
      "value": "2008-03-12",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}