DOI: 10.1111/j.1365-2141.1982.tb01886.x. The role of haem biosynthetic and degradative enzymes in erythroid colony development: the effect of haemin

The role of haem biosynthetic and degradative enzymes in erythroid colony development: the effect of haemin

10.1111/j.1365-2141.1982.tb01886.x

Crossref journal-article

Wiley

British Journal of Haematology (311)

Abstract

Summary. In vitro culture of murine bone marrow has proved to be a useful system for defining the haem biosynthetic and degradative enzymatic pathways during erythroid colony (CFU‐E) growth and development. Previous attempts to elucidate these pathways during erythropoiesis have been limited by the amount of biological material available as well as the sensitivity of specific enzyme assays. These conditions were overcome in the present study. In order to obtain an enrichment of CFU‐E, nonadherent mouse bone‐marrow cells were cultured in special culture plates using a modified methyl‐cellulose medium with erythropoietin. CFU‐E yields were increased approximately threefold. Utilizing the sensitive radiochemical assay, direct measurement of ALAS activity was determined in very early developing CFU‐E cultures as well as mature cultures. ALAS activity was found to reach a peak after 60 h of culture growth and then started to decline in activity. Cellular synthesis of haem was determined (with 14C ALA) and minor modifications of standard assays were also made in order to determine spectrophotometrically δ‐aminolaevulinic acid dehydratase (ALAD) and haem oxygenase activity in developing CFU‐E cultures. These assays were reproducible with as few as four or five culture plates. It was found that ALAD activity rose progressively after 36 h of culture growth and reached a plateau at about 60 h of growth. A continuous increase in 14C ALA incorporation into haem was seen at later hours of culture growth, suggesting that the induction of other haem enzymes beyond ALAS is still rate limiting in haem synthesis. Conversely, haem oxygenase activity declined up to 60 h of growth and was elevated at later culture periods without a subsequent increase in ALASA and ALAD. The observed increase in haem enzymes brought about by haemin was completely suppressed by addition of cycloheximide to the cultures. These results suggest that

Bibliography

Ibrahim, N. G., Lutton, J. D., & Levere, R. D. (1982). The role of haem biosynthetic and degradative enzymes in erythroid colony development: the effect of haemin. British Journal of Haematology, 50(1), 17â28. Portico.

Authors 3

Nader G. Ibrahim (first)
John D. Lutton (additional)
Richard D. Levere (additional)

References 37 Referenced 46

10.1172/JCI107680
10.1073/pnas.70.4.1022
10.1182/blood.V40.6.812.812 / Blood / Hemoglobin erythrocyte catabolism in rat liver. The separate roles of parenchymal and sinusoidal cells by Bissel O.M. (1972)
{'key': 'e_1_2_1_5_1', 'first-page': '509', 'article-title': 'Human hepatic δ‐aminolaevulinate synthase: requirement of an exogenous system for succinyl‐Co‐enzyme A generation to demonstrate increased activity in cirrhotic and anticonvulsant‐treated subjects', 'volume': '52', 'author': 'Bonkowsky H.L.', 'year': '1977', 'journal-title': 'Clinical Science and Molecular Medicine'} / Clinical Science and Molecular Medicine / Human hepatic δ‐aminolaevulinate synthase: requirement of an exogenous system for succinyl‐Co‐enzyme A generation to demonstrate increased activity in cirrhotic and anticonvulsant‐treated subjects by Bonkowsky H.L. (1977)
10.1016/0304-4165(78)90273-8
10.1016/S0021-9258(17)30261-2 / Journal of Biological Chemistry / Incorporation of exogenous heme into hepatic cytochrome P‐450 in vivo by Correia M.A. (1978)
10.1016/0003-9861(77)90092-3
10.1073/pnas.75.3.1148
10.3181/00379727-146-38155
10.1016/0304-4165(70)90242-4
10.1016/S0021-9258(19)42103-0 / Journal of Biological Chemistry / Hemin control of globin synthesis: isolation of a hemin‐reversible translational repressor from human mature erythrocytes by Freedman M.L. (1974)
10.1016/0014-5793(78)80816-3
10.1016/S0021-9258(17)30385-X / Journal of Biological Chemistry / Hemin control of heme biosynthesis in mouse friend virus‐transformed erythroleukemia cells in culture by Granick J.L. (1978)
10.1182/blood.V56.3.567.567 / Blood / Hemin control of heme biosynthesis and catabolism in human leukemia cell line by Hoffman R. (1980)
10.1016/S0021-9258(19)42129-7 / Journal of Biological Chemistry / The sensitivity of rat liver and yeast mitochondrial ribosome to inhibitor of protein synthesis by Ibrahim N.G. (1974)
{'key': 'e_1_2_1_17_1', 'first-page': '71', 'article-title': 'Role of iron in heme synthesis and degradation in rat bone marrow', 'volume': '52', 'author': 'Ibrahim N.G.', 'year': '1979', 'journal-title': 'Blood'} / Blood / Role of iron in heme synthesis and degradation in rat bone marrow by Ibrahim N.G. (1979)
10.1016/0024-3205(80)90315-X
10.1016/S0021-9258(19)78191-5 / Journal of Biological Chemistry / A sensitive radiochemical assay method for δ‐aminolevulinic acid synthetase by Irving E.A. (1969)
10.1002/jcp.1040830218
10.1038/274372a0
10.1111/j.1749-6632.1974.tb21891.x
10.1126/science.1257757
10.1016/S0021-9258(18)96086-2 / Journal of Biological Chemistry / Control of hemoglobin synthesis in the cultured chick blastoderm by Levere R.D. (1967)
10.1073/pnas.70.5.1526
10.1016/S0021-9258(17)40109-8 / Journal of Biological Chemistry / Solubilization and partial purification of heme oxygenase from rat liver by Maines M.D. (1977)
10.1038/243158a0
{'key': 'e_1_2_1_28_1', 'first-page': '434', 'article-title': 'The occurrence and determination of δ aminolevulinic and porphobilinogen in urine', 'volume': '259', 'author': 'Mauzerall D.', 'year': '1956', 'journal-title': 'Journal of Biological Chemistry'} / Journal of Biological Chemistry / The occurrence and determination of δ aminolevulinic and porphobilinogen in urine by Mauzerall D. (1956)
10.1172/JCI106697
{'key': 'e_1_2_1_30_1', 'first-page': '11', 'article-title': 'Enhancement of erythroid colony growth in culture by hemin', 'volume': '7', 'author': 'Porter P.N.', 'year': '1979', 'journal-title': 'Experimental Hematology'} / Experimental Hematology / Enhancement of erythroid colony growth in culture by hemin by Porter P.N. (1979)
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{'key': 'e_1_2_1_36_1', 'first-page': '410', 'article-title': 'The enzymatic catabolism of hemoglobin: stimulation of microsomal heme oxygenase by hemin', 'volume': '75', 'author': 'Tenhunen R.', 'year': '1970', 'journal-title': 'Journal of Laboratory and Clinical Medicine'} / Journal of Laboratory and Clinical Medicine / The enzymatic catabolism of hemoglobin: stimulation of microsomal heme oxygenase by hemin by Tenhunen R. (1970)
10.1073/pnas.75.8.3654
10.1016/0005-2744(80)90043-1

Dates

Type	When
Created	17 years, 5 months ago (March 12, 2008, 12:15 p.m.)
Deposited	1 year, 9 months ago (Nov. 11, 2023, 7:09 a.m.)
Indexed	1 month, 4 weeks ago (July 6, 2025, 1:02 p.m.)
Issued	43 years, 8 months ago (Jan. 1, 1982)
Published	43 years, 8 months ago (Jan. 1, 1982)
Published Online	17 years, 1 month ago (July 7, 2008)
Published Print	43 years, 8 months ago (Jan. 1, 1982)

Funders 0

None

BibTeX

@article{Ibrahim_1982, title={The role of haem biosynthetic and degradative enzymes in erythroid colony development: the effect of haemin}, volume={50}, ISSN={1365-2141}, url={http://dx.doi.org/10.1111/j.1365-2141.1982.tb01886.x}, DOI={10.1111/j.1365-2141.1982.tb01886.x}, number={1}, journal={British Journal of Haematology}, publisher={Wiley}, author={Ibrahim, Nader G. and Lutton, John D. and Levere, Richard D.}, year={1982}, month=jan, pages={17–28} }

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