DOI: 10.1111/j.1365-2141.1969.tb01408.x. Morphological‐Biochemical Correlations in Rabbit Red Cell Precursors Synchronized by Actinomycin Administration

Morphological‐Biochemical Correlations in Rabbit Red Cell Precursors Synchronized by Actinomycin Administration

10.1111/j.1365-2141.1969.tb01408.x

Crossref journal-article

Wiley

British Journal of Haematology (311)

Abstract

Summary. By making use of the selective toxicity of Actinomycin D, homogenous erythroid populations of various stages of maturation had been obtained in the rabbit. Comparison of the morphological characteristics of red cell precursors with in vitro synthetic activity as measured by the rate of incorporation of 32P, 59Fe and [2‐14C]glycine into DNA, RNA, haem and protein respectively, revealed the following:The rate of haem synthesis was negligible in pronormoblasts and early basophilic normoblasts, highest 1 day after Actinomycin administration, i.e. corresponding to a more advanced stage of erythroid maturation, and again lower in orthochromatic normoblasts.The rate of RNA synthesis was extremely low in the first 2 days following exposure to Actinomycin D. While our data could not permit distinction between interference of the drug with DNA‐dependent RNA synthesis and a spontaneous loss of RNA synthetic activity brought about by maturation, it was clear that this reduction in RNA synthesis did not affect the rate of protein synthesis. This observation lends further support to earlier claims for the existence of a stable messenger RNA in erythroid cells.Proliferation of undifferentiated mononuclear cells preceded the restitution of bone marrow population after the toxic action of Actinomycin D. Factors affecting their in vitro differentiation are currently being investigated in our laboratory.

Bibliography

Hershko, Ch., Schwartz, R., & Izak, G. (1969). MorphologicalâBiochemical Correlations in Rabbit Red Cell Precursors Synchronized by Actinomycin Administration. British Journal of Haematology, 17(6), 569â579. Portico.

Authors 3

Ch. Hershko (first)
R. Schwartz (additional)
G. Izak (additional)

References 24 Referenced 9

10.1073/pnas.57.6.1607
{'key': 'e_1_2_1_3_1', 'first-page': '285', 'volume-title': 'Nucleic Acid', 'author': 'Dische Z.', 'year': '1955'} / Nucleic Acid by Dische Z. (1955)
10.1016/0005-2787(65)90171-1
10.2307/3571480
Izak G. Karsai A. Eylon L.andSchiffman A.(1969). ‘Radiation‐induced changes in some biochemical parameters of the haemopoietic tissue of rabbits.Israel J. med. Sci. (in press).
10.1182/blood.V27.5.637.637 / Blood / Actinomycin and erythropoiesis and the production of erythropoietin in mice by Keighley G. (1966)
10.1016/0005-2787(65)90172-3
10.1152/physrev.1957.37.1.50
10.1016/S0096-4174(18)30210-5
10.1111/j.1365-2141.1955.tb05488.x
10.1016/0006-291X(67)90116-7
10.1182/blood.V31.4.433.433 / Blood / In vitro effect of erythropoietin on the spleen of the polycythemic mouse. III. Limited exposure to erythropoietin and actinomycin D by Miura Y. (1968)
{'key': 'e_1_2_1_14_1', 'first-page': '163', 'article-title': 'Release of tyrosinase activity in a vertebrate cell system by Actinomycin D.’ (Abstract)', 'volume': '21', 'author': 'Peck D.', 'year': '1962', 'journal-title': 'Fed. Proc.'} / Fed. Proc. / Release of tyrosinase activity in a vertebrate cell system by Actinomycin D.’ (Abstract) by Peck D. (1962)
10.1111/j.1749-6632.1960.tb20158.x
10.1016/0024-3205(67)90140-3
{'key': 'e_1_2_1_17_1', 'first-page': '1428', 'article-title': 'Biochemistry of actinomycin', 'volume': '23', 'author': 'Reich E.', 'year': '1963', 'journal-title': 'Cancer Res.'} / Cancer Res. / Biochemistry of actinomycin by Reich E. (1963)
10.1182/blood.V28.2.201.201 / Blood / Selective eradication of erythropoiesis by actinomycin D as the result of interference with hormonally controlled effect or pathway of cell differentiation by Reissmann K. R. (1966)
10.1111/j.1749-6632.1968.tb15152.x
10.1126/science.146.3644.661
{'key': 'e_1_2_1_21_1', 'first-page': '337', 'article-title': 'The effect of mitochondria on porphyrin and heme biosynthesis in the red blood cells', 'volume': '21', 'author': 'Sano S.', 'year': '1958', 'journal-title': 'Acta haemat. jap.'} / Acta haemat. jap. / The effect of mitochondria on porphyrin and heme biosynthesis in the red blood cells by Sano S. (1958)
10.1111/j.1749-6632.1968.tb15156.x
10.1016/S0076-6879(57)03442-4
10.1182/blood.V5.7.597.597 / Blood / The supravital method in the study of the cytology of blood and marrow cells by Schwind J. L. (1950)
10.1016/0009-8981(65)90222-6

Dates

Type	When
Created	17 years, 5 months ago (March 12, 2008, 9:53 a.m.)
Deposited	1 year, 9 months ago (Nov. 11, 2023, 4:36 a.m.)
Indexed	1 year, 9 months ago (Nov. 12, 2023, 9:52 p.m.)
Issued	55 years, 8 months ago (Dec. 1, 1969)
Published	55 years, 8 months ago (Dec. 1, 1969)
Published Online	17 years, 5 months ago (March 12, 2008)
Published Print	55 years, 8 months ago (Dec. 1, 1969)

Funders 0

None

BibTeX

@article{Hershko_1969, title={Morphological‐Biochemical Correlations in Rabbit Red Cell Precursors Synchronized by Actinomycin Administration}, volume={17}, ISSN={1365-2141}, url={http://dx.doi.org/10.1111/j.1365-2141.1969.tb01408.x}, DOI={10.1111/j.1365-2141.1969.tb01408.x}, number={6}, journal={British Journal of Haematology}, publisher={Wiley}, author={Hershko, Ch. and Schwartz, R. and Izak, G.}, year={1969}, month=dec, pages={569–579} }

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