DOI: 10.1111/j.1349-7006.1999.tb00712.x. β‐Catenin Accumulation and Mutation of Exon 3 of the β‐<i>Catenin</i> Gene in Hepatocellular Carcinoma

β‐Catenin Accumulation and Mutation of Exon 3 of the β‐Catenin Gene in Hepatocellular Carcinoma

10.1111/j.1349-7006.1999.tb00712.x

Crossref journal-article

Wiley

Japanese Journal of Cancer Research (311)

Abstract

study was conducted to clarify the contribution of β‐catenin accumulation and mutation of the β‐catenin gene to hepatocarcinogenesis. β‐Catenin accumulation was examined immunohistochemically in 38 paired samples of hepatocellular carcinoma (HCC) and corresponding non‐cancerous liver tissue. Gene mutation was analyzed by polymerase chain reaction‐single strand conformation polymorphism (PCR‐SSCP) and direct sequencing using intronic primers encompassing exon 3. Neither accumulation nor mutation was detected in non‐cancerous liver tissues that showed no remarkable histological features, chronic hepatitis or liver cirrhosis. Accumulation of β‐catenin was seen in the nucleus, cytoplasm or cell membrane in 15 of 38 (39%) HCC samples, and gene mutation was seen in 9 of 38 (24%) HCC samples. Although there was a significant correlation between accumulation and mutation (P<0.01), six HCCs without mutation also showed accumulation. Samples of early HCC showed neither accumulation nor mutation, and accumulation and mutation were each correlated significantly with portal vein tumor involvement (P<0.05). The present results indicate that (1) mutation of exon 3 of the β‐catenin gene can lead to β‐catenin accumulation, although other mechanisms of accumulation may also operate in HCC, and (2) β‐catenin accumulation and mutation of the β‐catenin gene are not early events in hepatocarcinogenesis, and may be associated with the malignant progression of HCC.

Bibliography

Kondo, Y., Kanai, Y., Sakamoto, M., Genda, T., Mizokami, M., Ueda, R., & Hirohashi, S. (1999). Î²âCatenin Accumulation and Mutation of Exon 3 of the Î²âCatenin Gene in Hepatocellular Carcinoma. Japanese Journal of Cancer Research, 90(12), 1301â1309. Portico.

Authors 7

Yutaka Kondo (first)
Yae Kanai (additional)
Michiie Sakamoto (additional)
Takuya Genda (additional)
Masashi Mizokami (additional)
Ryuzo Ueda (additional)
Setsuo Hirohashi (additional)

References 26 Referenced 74

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{'key': 'e_1_2_1_10_2', 'first-page': '1021', 'article-title': 'Activation of the β‐catenin gene by interstitial deletions involving exon 3 in primary colorectal carcinomas without adenomatous polyposis coli mutations', 'volume': '58', 'author': 'Iwao K.', 'year': '1998', 'journal-title': 'Cancer Res.'} / Cancer Res. / Activation of the β‐catenin gene by interstitial deletions involving exon 3 in primary colorectal carcinomas without adenomatous polyposis coli mutations by Iwao K. (1998)
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{'key': 'e_1_2_1_12_2', 'first-page': '2524', 'article-title': 'Activation of the β‐catenin gene in primary hepatocellular carcinomas by somatic alterations involving exon 3', 'volume': '58', 'author': 'Miyoshi Y.', 'year': '1998', 'journal-title': 'Cancer Res.'} / Cancer Res. / Activation of the β‐catenin gene in primary hepatocellular carcinomas by somatic alterations involving exon 3 by Miyoshi Y. (1998)
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Dates

Type	When
Created	20 years ago (Aug. 23, 2005, 4:57 a.m.)
Deposited	1 year, 10 months ago (Oct. 28, 2023, 8:54 a.m.)
Indexed	1 day, 10 hours ago (Sept. 3, 2025, 6:52 a.m.)
Issued	25 years, 9 months ago (Dec. 1, 1999)
Published	25 years, 9 months ago (Dec. 1, 1999)
Published Online	20 years ago (Aug. 23, 2005)
Published Print	25 years, 9 months ago (Dec. 1, 1999)

Funders 0

None

BibTeX

@article{Kondo_1999, title={β‐Catenin Accumulation and Mutation of Exon 3 of the β‐Catenin Gene in Hepatocellular Carcinoma}, volume={90}, ISSN={0910-5050}, url={http://dx.doi.org/10.1111/j.1349-7006.1999.tb00712.x}, DOI={10.1111/j.1349-7006.1999.tb00712.x}, number={12}, journal={Japanese Journal of Cancer Research}, publisher={Wiley}, author={Kondo, Yutaka and Kanai, Yae and Sakamoto, Michiie and Genda, Takuya and Mizokami, Masashi and Ueda, Ryuzo and Hirohashi, Setsuo}, year={1999}, month=dec, pages={1301–1309} }

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