DOI: 10.1111/j.1349-7006.1998.tb00583.x. Clotrimazole, an Imidazole Antimycotic, Is a Potent Inhibitor of Angiogenesis

Clotrimazole, an Imidazole Antimycotic, Is a Potent Inhibitor of Angiogenesis

10.1111/j.1349-7006.1998.tb00583.x

Crossref journal-article

Wiley

Japanese Journal of Cancer Research (311)

Abstract

Clotrimazole, an imidazole antimycotic, interferes with the rise in cytosolic Ca2+ and inhibits cell proliferation in a reversible manner. Here we describe the effect of clotrimazole on vascular endothelial cells (ECs). Clotrimazole inhibited the proliferation of ECs stimulated with typical angiogenic growth factors; vascular endothelial growth factor and basic fibroblast growth factor (bFGF). This inhibitory effect of clotrimazole was dose‐dependent and the maximal inhibition was observed at a concentration of 10 mM. We did not observe any increase in 51Cr release from ECs during treatment with 10 μM. clotrimazole. Moreover, clotrimazole inhibited the basal and bFGF‐stimulated migration of ECs. As clotrimazole inhibited two principle components of angiogenesis; the proliferation and migration of ECs, we examined whether clotrimazole inhibited angiogenesis. Tube formation by ECs in type 1 collagen gel was investigated, and clotrimazole was found to be significantly inhibitory. The inhibitory effect of clotrimazole on angiogenesis was further confirmed in an in vivo angiogenesis model of murine Matrigel plug assay. These results demonstrate that clotrimazole is a potent inhibitor of angiogenesis.

Bibliography

Takahashi, H., Abe, M., Sugawara, T., Tanaka, K., Saito, Y., Fujimura, S., Shibuya, M., & Sato, Y. (1998). Clotrimazole, an Imidazole Antimycotic, Is a Potent Inhibitor of Angiogenesis. Japanese Journal of Cancer Research, 89(4), 445â451. Portico.

Authors 8

Hiroto Takahashi (first)
Mayumi Abe (additional)
Toshiyuki Sugawara (additional)
Katsuhiro Tanaka (additional)
Yasuki Saito (additional)
Sigefumi Fujimura (additional)
Masabumi Shibuya (additional)
Yasufumi Sato (additional)

References 22 Referenced 28

10.1016/S0021-9258(19)49853-0
10.1093/jnci/82.1.4
10.1042/bj2770073
10.1096/fasebj.6.9.1319362
10.1016/S0021-9258(19)49767-6 / J. Biol. Chem. / High affinity inhibition of Ca2+‐dependent K+ channels by cytochrome P‐450 inhibitors by Alvarez J. (1992)
10.1038/nm0695-534
{'key': 'e_1_2_1_8_2', 'first-page': '213', 'article-title': 'FLT‐1 but not KDR/FLK‐1 is a receptor for placenta growth factor, which is related to vascular endothelial growth factor', 'volume': '7', 'author': 'Sawano A.', 'year': '1996', 'journal-title': 'Cell Growth Differ.'} / Cell Growth Differ. / FLT‐1 but not KDR/FLK‐1 is a receptor for placenta growth factor, which is related to vascular endothelial growth factor by Sawano A. (1996)
10.1016/S0006-291X(05)81179-9
10.1016/0006-291X(90)90715-Y
10.1083/jcb.123.5.1249
10.1016/0952-3278(88)90028-2
10.1083/jcb.107.3.1199
10.1006/excr.1993.1028
{'key': 'e_1_2_1_15_2', 'first-page': '519', 'article-title': 'A simple, quantitative method for assessing angiogenesis and antiangiogenic agents using reconstituted basement membrane, heparin, and fibroblast growth factor', 'volume': '67', 'author': 'Passaniti A.', 'year': '1992', 'journal-title': 'Lab. Invest.'} / Lab. Invest. / A simple, quantitative method for assessing angiogenesis and antiangiogenic agents using reconstituted basement membrane, heparin, and fibroblast growth factor by Passaniti A. (1992)
10.1083/jcb.109.4.1877
10.1073/pnas.92.5.1307
{'key': 'e_1_2_1_18_2', 'first-page': '672', 'article-title': 'Angiogenesis inhibition by minocycline', 'volume': '51', 'author': 'Tamargo R. J.', 'year': '1991', 'journal-title': 'Cancer Res.'} / Cancer Res. / Angiogenesis inhibition by minocycline by Tamargo R. J. (1991)
10.1016/0014-5793(93)81558-H
{'key': 'e_1_2_1_20_2', 'first-page': '1512', 'article-title': 'Inhibition of tumor growth and neovascularization by an anti‐gastric ulcer agent, irsogladine', 'volume': '56', 'author': 'Ono M.', 'year': '1996', 'journal-title': 'Cancer Res.'} / Cancer Res. / Inhibition of tumor growth and neovascularization by an anti‐gastric ulcer agent, irsogladine by Ono M. (1996)
10.1172/JCI118838
10.1161/01.CIR.94.10.2566
10.1055/s-0028-1111224

Dates

Type	When
Created	20 years ago (Aug. 23, 2005, 10:40 a.m.)
Deposited	1 year, 10 months ago (Oct. 27, 2023, 9:57 p.m.)
Indexed	3 months, 1 week ago (May 26, 2025, 9:05 a.m.)
Issued	27 years, 5 months ago (April 1, 1998)
Published	27 years, 5 months ago (April 1, 1998)
Published Online	20 years ago (Aug. 23, 2005)
Published Print	27 years, 5 months ago (April 1, 1998)

Funders 0

None

BibTeX

@article{Takahashi_1998, title={Clotrimazole, an Imidazole Antimycotic, Is a Potent Inhibitor of Angiogenesis}, volume={89}, ISSN={0910-5050}, url={http://dx.doi.org/10.1111/j.1349-7006.1998.tb00583.x}, DOI={10.1111/j.1349-7006.1998.tb00583.x}, number={4}, journal={Japanese Journal of Cancer Research}, publisher={Wiley}, author={Takahashi, Hiroto and Abe, Mayumi and Sugawara, Toshiyuki and Tanaka, Katsuhiro and Saito, Yasuki and Fujimura, Sigefumi and Shibuya, Masabumi and Sato, Yasufumi}, year={1998}, month=apr, pages={445–451} }

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