DOI: 10.1111/j.1349-7006.1995.tb02457.x. Changes in Radiation Sensitivity of Human Osteosarcoma Cells after p53 Introduction

Changes in Radiation Sensitivity of Human Osteosarcoma Cells after p53 Introduction

10.1111/j.1349-7006.1995.tb02457.x

Crossref journal-article

Wiley

Japanese Journal of Cancer Research (311)

Abstract

Human osteosarcoma SAOS‐2 cells, which have a deletion in p53 gene, were transfected with plasmid pMSVneop53 containing human p53 cDNA and neomycin‐resistance gene. Three clones (SAOS‐MC10, SAOS‐MC11 and SAOS‐MC43) among 60 clones expressed p53 mRNA. No p53 protein was observed in SAOS‐MC10, while SAOS‐MC11 and SAOS‐MC43 produced p53 protein. The molecular weight of p53 protein in SAOS‐MC43 was lower than that in SAOS‐MC11. SAOS‐MC11 and SAOS‐MC43 were more sensitive and more resistant, respectively, to ionizing radiation than the parental SAOS‐2. We suggest that exogenous p53 protein might be one of the factors determining cellular radiosensitivity.

Bibliography

Miyakoshi, J., Yamagishi, N., Ohtsu, S., & Takebe, H. (1995). Changes in Radiation Sensitivity of Human Osteosarcoma Cells after p53 Introduction. Japanese Journal of Cancer Research, 86(8), 711â713. Portico.

Authors 4

Junji Miyakoshi (first)
Nobuyuki Yamagishi (additional)
Shuji Ohtsu (additional)
Hiraku Takebe (additional)

References 8 Referenced 3

10.1126/science.1905840
10.1101/gad.4.1.1
10.1073/pnas.89.16.7491
10.1038/362849a0
10.1038/362847a0
{'key': 'e_1_2_1_7_2', 'first-page': '4776', 'article-title': "Role of the p53 tumor suppressor gene in cell cycle arrest and radiosensitivity of Burkitt's lymphoma cell lines", 'volume': '53', 'author': "O'Connor P. M.", 'year': '1993', 'journal-title': 'Cancer Res.'} / Cancer Res. / Role of the p53 tumor suppressor gene in cell cycle arrest and radiosensitivity of Burkitt's lymphoma cell lines by O'Connor P. M. (1993)
10.1073/pnas.84.21.7716
10.1128/MCB.10.11.5772

Dates

Type	When
Created	20 years ago (Aug. 24, 2005, 4:15 a.m.)
Deposited	1 year, 10 months ago (Oct. 26, 2023, 10:15 a.m.)
Indexed	2 months, 3 weeks ago (June 11, 2025, 5:29 a.m.)
Issued	30 years, 1 month ago (Aug. 1, 1995)
Published	30 years, 1 month ago (Aug. 1, 1995)
Published Online	20 years ago (Aug. 24, 2005)
Published Print	30 years, 1 month ago (Aug. 1, 1995)

Funders 0

None

BibTeX

@article{Miyakoshi_1995, title={Changes in Radiation Sensitivity of Human Osteosarcoma Cells after p53 Introduction}, volume={86}, ISSN={0910-5050}, url={http://dx.doi.org/10.1111/j.1349-7006.1995.tb02457.x}, DOI={10.1111/j.1349-7006.1995.tb02457.x}, number={8}, journal={Japanese Journal of Cancer Research}, publisher={Wiley}, author={Miyakoshi, Junji and Yamagishi, Nobuyuki and Ohtsu, Shuji and Takebe, Hiraku}, year={1995}, month=aug, pages={711–713} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        6,
        11
      ]
    ],
    "date-time": "2025-06-11T09:29:07Z",
    "timestamp": 1749634147528
  },
  "reference-count": 8,
  "publisher": "Wiley",
  "issue": "8",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2005,
            8,
            24
          ]
        ],
        "date-time": "2005-08-24T00:00:00Z",
        "timestamp": 1124841600000
      },
      "content-version": "vor",
      "delay-in-days": 3676,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1995,
        8
      ]
    ]
  },
  "abstract": "<jats:p>Human osteosarcoma SAOS\u20102 cells, which have a deletion in p53 gene, were transfected with plasmid pMSVneop53 containing human p53 cDNA and neomycin\u2010resistance gene. Three clones (SAOS\u2010MC10, SAOS\u2010MC11 and SAOS\u2010MC43) among 60 clones expressed p53 mRNA. No p53 protein was observed in SAOS\u2010MC10, while SAOS\u2010MC11 and SAOS\u2010MC43 produced p53 protein. The molecular weight of p53 protein in SAOS\u2010MC43 was lower than that in SAOS\u2010MC11. SAOS\u2010MC11 and SAOS\u2010MC43 were more sensitive and more resistant, respectively, to ionizing radiation than the parental SAOS\u20102. We suggest that exogenous p53 protein might be one of the factors determining cellular radiosensitivity.</jats:p>",
  "DOI": "10.1111/j.1349-7006.1995.tb02457.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2005,
        8,
        24
      ]
    ],
    "date-time": "2005-08-24T08:15:48Z",
    "timestamp": 1124871348000
  },
  "page": "711-713",
  "source": "Crossref",
  "is-referenced-by-count": 3,
  "title": "Changes in Radiation Sensitivity of Human Osteosarcoma Cells after p53 Introduction",
  "prefix": "10.1111",
  "volume": "86",
  "author": [
    {
      "given": "Junji",
      "family": "Miyakoshi",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Nobuyuki",
      "family": "Yamagishi",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Shuji",
      "family": "Ohtsu",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Hiraku",
      "family": "Takebe",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2005,
        8,
        24
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1905840"
    },
    {
      "key": "e_1_2_1_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.4.1.1"
    },
    {
      "key": "e_1_2_1_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.89.16.7491"
    },
    {
      "key": "e_1_2_1_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/362849a0"
    },
    {
      "key": "e_1_2_1_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/362847a0"
    },
    {
      "key": "e_1_2_1_7_2",
      "first-page": "4776",
      "article-title": "Role of the p53 tumor suppressor gene in cell cycle arrest and radiosensitivity of Burkitt's lymphoma cell lines",
      "volume": "53",
      "author": "O'Connor P. M.",
      "year": "1993",
      "journal-title": "Cancer Res."
    },
    {
      "key": "e_1_2_1_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.84.21.7716"
    },
    {
      "key": "e_1_2_1_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.10.11.5772"
    }
  ],
  "container-title": "Japanese Journal of Cancer Research",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1349-7006.1995.tb02457.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1349-7006.1995.tb02457.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        10,
        26
      ]
    ],
    "date-time": "2023-10-26T14:15:26Z",
    "timestamp": 1698329726000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1111/j.1349-7006.1995.tb02457.x"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1995,
        8
      ]
    ]
  },
  "references-count": 8,
  "journal-issue": {
    "issue": "8",
    "published-print": {
      "date-parts": [
        [
          1995,
          8
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1111/j.1349-7006.1995.tb02457.x"
  ],
  "URL": "http://dx.doi.org/10.1111/j.1349-7006.1995.tb02457.x",
  "relation": {},
  "ISSN": [
    "0910-5050"
  ],
  "subject": [],
  "container-title-short": "Japanese Journal of Cancer Research",
  "published": {
    "date-parts": [
      [
        1995,
        8
      ]
    ]
  }
}