DOI: 10.1111/j.1348-0421.2004.tb03547.x. Development and Evaluation of a Rapid Flow‐Through Immuno Filtration Test Using Recombinant Filarial Antigen for Diagnosis of Brugian and Bancroftian Filariasis

Development and Evaluation of a Rapid Flow‐Through Immuno Filtration Test Using Recombinant Filarial Antigen for Diagnosis of Brugian and Bancroftian Filariasis

10.1111/j.1348-0421.2004.tb03547.x

Crossref journal-article

Wiley

Microbiology and Immunology (311)

Abstract

AbstractThere is an imperative need to develop a rapid antibody test that can be used for diagnosis of clinical cases in travelers and expatriates, primary surveillance in areas of unknown endemicity, detection of early infection in childhood and for monitoring chemotherapeutic programs. A rapid‐format, simple and qualitative flow through immuno filtration test has been developed for the identification of total IgG antibodies to recombinant filarial antigen WbSXP‐1. This test system employs colloidal gold‐protein A reagent as the antibody capture reagent. The sensitivity and specificity of the test was evaluated in a total of 1,230 serum samples. The sensitivity of the test was found to be 90.8% with brugian (n=70) and 91.4% with bancroftian (n = 140) microfilaraemic subjects. The test showed minimum reactivity (4/10) with Loa loa microfilaria (MF) positive sera and no reactivity (0/20) with Onchocerca MF positive sera. This rapid diagnosis is found to be non‐reactive with individuals having other parasitic diseases including schistosomiasis (n = 10), soil‐transmitted helminthiases (n=34) and protozoan infections (n=33) indicating the potential of this test as a prospective method of diagnosis for both brugian and bancroftian lymphatic filariasis. Stability kinetics was studied at different temperatures and different time periods. The rapid flow‐through immuno filtration test is advantageous since it can be stored at room temperature, is user friendly and is particularly applicable in the field as an initial screening method, for epidemiological monitoring of filarial infections in bancroftian and brugian endemic regions of the world.

Bibliography

Baskar, L. K. V., Srikanth, T. R., Suba, S., Mody, H. C., Desai, P. K., & Kaliraj, P. (2004). Development and Evaluation of a Rapid FlowâThrough Immuno Filtration Test Using Recombinant Filarial Antigen for Diagnosis of Brugian and Bancroftian Filariasis. Microbiology and Immunology, 48(7), 519â525. Portico.

Authors 6

Lakshmi Kanthan Vijaya Baskar (first)
Tokuluva Ramalingam Srikanth (additional)
Salahudeen Suba (additional)
Himansu Chirag Mody (additional)
Pradeep Kumar Desai (additional)
Perumal Kaliraj (additional)

References 28 Referenced 28

{'key': 'e_1_2_2_2_1', 'first-page': '246', 'article-title': 'High levels of parasite‐specific IgG4 in the absence of microfilaremia in Loa loa infection', 'volume': '45', 'author': 'Akue J.P.', 'year': '1994', 'journal-title': 'Trop. Med. Parasitol.'} / Trop. Med. Parasitol. / High levels of parasite‐specific IgG4 in the absence of microfilaremia in Loa loa infection by Akue J.P. (1994)
10.1006/expr.1993.1101
10.1016/0166-6851(94)00035-2
10.1007/BF02779018
10.1016/0166-6851(92)90176-K
10.1038/physci241020a0
10.1046/j.1365-3156.1999.00367.x
10.1086/345873
{'key': 'e_1_2_2_10_1', 'first-page': '4298', 'article-title': 'Differential expression of IgE and IgG4‐specific antibody responses in asymptomatic and chronic human filariasis', 'volume': '145', 'author': 'Kurniawan A.', 'year': '1993', 'journal-title': 'J. Immunol.'} / J. Immunol. / Differential expression of IgE and IgG4‐specific antibody responses in asymptomatic and chronic human filariasis by Kurniawan A. (1993)
10.1046/j.1365-3156.1998.00170.x
10.1111/j.1348-0421.2002.tb02703.x
10.1093/infdis/172.2.521
10.1016/S0169-4758(97)01151-4
{'key': 'e_1_2_2_15_1', 'first-page': '403', 'article-title': 'A highly specific and sensitive monoclonal antibody based ELISA for the detection of circulating antigen in bancrofti filariasis', 'volume': '41', 'author': 'More S.J.', 'year': '1990', 'journal-title': 'Trop. Med. Parasitol.'} / Trop. Med. Parasitol. / A highly specific and sensitive monoclonal antibody based ELISA for the detection of circulating antigen in bancrofti filariasis by More S.J. (1990)
{'key': 'e_1_2_2_16_1', 'first-page': '97', 'article-title': 'Studies on the nocturnal periodicity of Wuchereria bancrofti and Brugia malayi microfilariae in Kerala state', 'volume': '4', 'author': 'Nair C.P.', 'year': '1972', 'journal-title': 'J. Commun. Dis.'} / J. Commun. Dis. / Studies on the nocturnal periodicity of Wuchereria bancrofti and Brugia malayi microfilariae in Kerala state by Nair C.P. (1972)
10.1046/j.1365-3156.2000.00620.x
10.1046/j.1365-3156.1998.00229.x
10.1016/S0035-9203(01)90234-2
10.1016/S0035-9203(01)90091-4
{'key': 'e_1_2_2_21_1', 'first-page': '313', 'article-title': 'Current status of filariasis in Chavakad taluk, Trichur district, Kerala', 'volume': '29', 'author': 'Rajendran G.', 'year': '1997', 'journal-title': 'J. Commun. Dis.'} / J. Commun. Dis. / Current status of filariasis in Chavakad taluk, Trichur district, Kerala by Rajendran G. (1997)
{'key': 'e_1_2_2_22_1', 'first-page': '4', 'volume-title': 'Filarial Links', 'author': 'Ramachandran C.', 'year': '1997'} / Filarial Links by Ramachandran C. (1997)
10.1016/S0166-6851(99)00231-5
10.1016/S0035-9203(96)90140-6
10.1093/infdis/165.6.1158
10.1016/S0169-4758(97)01130-7
10.1046/j.1365-3156.2001.00765.x
World Health Organization.1992.Informal consultation on evaluation of morbidity in lymphatic filariasis Madras. Mimeographed document WHO/TDR/FIK/92.3.1.
{'key': 'e_1_2_2_29_1', 'first-page': '3', 'volume': '1', 'author': 'World Health Organization, Division of Control of Tropical Diseases', 'year': '1998', 'journal-title': 'Lymphatic filariasis; reasons of hope'} / Lymphatic filariasis; reasons of hope by World Health Organization, Division of Control of Tropical Diseases (1998)

Dates

Type	When
Created	11 years, 9 months ago (Nov. 14, 2013, 10:15 a.m.)
Deposited	1 year, 9 months ago (Nov. 20, 2023, 6:20 p.m.)
Indexed	1 year, 2 months ago (June 13, 2024, 1:17 a.m.)
Issued	21 years, 2 months ago (July 1, 2004)
Published	21 years, 2 months ago (July 1, 2004)
Published Online	11 years, 9 months ago (Nov. 14, 2013)
Published Print	21 years, 2 months ago (July 1, 2004)

Funders 0

None

BibTeX

@article{Baskar_2004, title={Development and Evaluation of a Rapid Flow‐Through Immuno Filtration Test Using Recombinant Filarial Antigen for Diagnosis of Brugian and Bancroftian Filariasis}, volume={48}, ISSN={1348-0421}, url={http://dx.doi.org/10.1111/j.1348-0421.2004.tb03547.x}, DOI={10.1111/j.1348-0421.2004.tb03547.x}, number={7}, journal={Microbiology and Immunology}, publisher={Wiley}, author={Baskar, Lakshmi Kanthan Vijaya and Srikanth, Tokuluva Ramalingam and Suba, Salahudeen and Mody, Himansu Chirag and Desai, Pradeep Kumar and Kaliraj, Perumal}, year={2004}, month=jul, pages={519–525} }

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