DOI: 10.1111/j.1348-0421.1996.tb03338.x. Fgr Expression Restricted to Subpopulation of Monocyte/Macrophage Lineage in Resting Conditions Is Induced in Various Hematopoietic Cells after Activation or Transformation

Fgr Expression Restricted to Subpopulation of Monocyte/Macrophage Lineage in Resting Conditions Is Induced in Various Hematopoietic Cells after Activation or Transformation

10.1111/j.1348-0421.1996.tb03338.x

Crossref journal-article

Wiley

Microbiology and Immunology (311)

Abstract

AbstractThe c‐fgr gene product (Fgr) is a member of the src‐family of protein tyrosine kinases. We have established a monoclonal antibody (2H2) which recognizes the unique N‐terminal domain of the murine Fgr. In the present study, using immunohistochemical analysis and immune complex kinase assay with the 2H2, we investigated expression of Fgr in various cell populations and tissues in a murine system. In resting conditions, Fgr expression was confined to subsets of a monocyte/macrophage lineage. Thus, Fgr+ cells were detected in paracortical areas and medullas of lymph nodes, but seen only in marginal zones of the spleen and the medulla of the thymus. No Fgr+ macrophage was detected in other tissues, Peyer's patches, brain, heart, lung, liver, pancreas, kidney and peritoneal cavity. However, immune complex kinase assay revealed that, upon stimulation, T and B cells as well as peritoneal macrophages expressed significant levels of Fgr molecules. Transformed cell lines of lymphoid origin, EL‐4 and LK35.2, which are T and B lineage lymphomas, respectively, also expressed Fgr molecules. Thus, various cells of hematopoietic origin appeared to possess a potentiality to express Fgr following activation or transformation. The present findings may help elucidate the functional significance of Fgr in immunologically committed cells in either activated or non‐activated conditions.

Bibliography

Hatakeyama, S., Iwabuchi, K., Ato, M., Iwabuchi, C., Kajino, K., Takami, K., Katoh, M., Ogasawara, K., Good, R. A., & OnoÃ©, K. (1996). Fgr Expression Restricted to Subpopulation of Monocyte/Macrophage Lineage in Resting Conditions Is Induced in Various Hematopoietic Cells after Activation or Transformation. Microbiology and Immunology, 40(3), 223â231. Portico.

Authors 10

Shigetsugu Hatakeyama (first)
Kazuya Iwabuchi (additional)
Manabu Ato (additional)
Chikako Iwabuchi (additional)
Kiichi Kajino (additional)
Kimitaka Takami (additional)
Masahito Katoh (additional)
Kazumasa Ogasawara (additional)
Robert A. Good (additional)
Kazunori Onoé (additional)

References 37 Referenced 11

10.4049/jimmunol.151.2.546 / J. Immunol. / Lymphokine activated killer cell activity of CD4−8−TCRαβ+ thymocytes by Arase H. (1993)
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{'key': 'e_1_2_2_4_1', 'first-page': '2025', 'article-title': 'Non‐receptor tyrosine protein kinases', 'volume': '8', 'author': 'Bolen J.', 'year': '1993', 'journal-title': 'Oncogene'} / Oncogene / Non‐receptor tyrosine protein kinases by Bolen J. (1993)
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{'key': 'e_1_2_2_9_1', 'first-page': '23', 'article-title': 'Marginal zone macrophages identified by a monoclonal antibody: characterization of immuno‐ and enzyme‐histochemical properties and functional capacities', 'volume': '55', 'author': 'Dijkstra C.D.', 'year': '1985', 'journal-title': 'Immunology'} / Immunology / Marginal zone macrophages identified by a monoclonal antibody: characterization of immuno‐ and enzyme‐histochemical properties and functional capacities by Dijkstra C.D. (1985)
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{'key': 'e_1_2_2_17_1', 'first-page': '391', 'article-title': 'Sequential analysis of distribution of donor‐derived thymocytes bearing T cell antigen receptor (TCR) and donor‐derived Ia+ cells in thymuses of fully allogeneic bone marrow chimera in mice', 'volume': '40', 'author': 'Hatakeyama S.', 'year': '1990', 'journal-title': 'Acta Pathol. Jpn.'} / Acta Pathol. Jpn. / Sequential analysis of distribution of donor‐derived thymocytes bearing T cell antigen receptor (TCR) and donor‐derived Ia+ cells in thymuses of fully allogeneic bone marrow chimera in mice by Hatakeyama S. (1990)
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{'key': 'e_1_2_2_20_1', 'first-page': '301', 'article-title': 'Isolation and sequencing of cDNA clones homologous to the v‐fgr oncogene from a human B lymphocyte cell line, IM‐9', 'volume': '1', 'author': 'Inoue K.', 'year': '1987', 'journal-title': 'Oncogene'} / Oncogene / Isolation and sequencing of cDNA clones homologous to the v‐fgr oncogene from a human B lymphocyte cell line, IM‐9 by Inoue K. (1987)
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{'key': 'e_1_2_2_30_1', 'first-page': '877', 'article-title': 'Characterization of the 5′ untranslated region of the c‐fgr gene and identification of the major myelomonocytic c‐fgr promoter', 'volume': '7', 'author': 'Link D.C.', 'year': '1992', 'journal-title': 'Oncogene'} / Oncogene / Characterization of the 5′ untranslated region of the c‐fgr gene and identification of the major myelomonocytic c‐fgr promoter by Link D.C. (1992)
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Dates

Type	When
Created	11 years, 9 months ago (Nov. 14, 2013, 11:51 a.m.)
Deposited	1 year, 9 months ago (Nov. 20, 2023, 3:55 p.m.)
Indexed	1 week, 2 days ago (Aug. 23, 2025, 9:57 p.m.)
Issued	29 years, 6 months ago (March 1, 1996)
Published	29 years, 6 months ago (March 1, 1996)
Published Online	11 years, 9 months ago (Nov. 14, 2013)
Published Print	29 years, 6 months ago (March 1, 1996)

Funders 0

None

BibTeX

@article{Hatakeyama_1996, title={Fgr Expression Restricted to Subpopulation of Monocyte/Macrophage Lineage in Resting Conditions Is Induced in Various Hematopoietic Cells after Activation or Transformation}, volume={40}, ISSN={1348-0421}, url={http://dx.doi.org/10.1111/j.1348-0421.1996.tb03338.x}, DOI={10.1111/j.1348-0421.1996.tb03338.x}, number={3}, journal={Microbiology and Immunology}, publisher={Wiley}, author={Hatakeyama, Shigetsugu and Iwabuchi, Kazuya and Ato, Manabu and Iwabuchi, Chikako and Kajino, Kiichi and Takami, Kimitaka and Katoh, Masahito and Ogasawara, Kazumasa and Good, Robert A. and Onoé, Kazunori}, year={1996}, month=mar, pages={223–231} }

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        3
      ]
    ]
  },
  "references-count": 37,
  "journal-issue": {
    "issue": "3",
    "published-print": {
      "date-parts": [
        [
          1996,
          3
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1111/j.1348-0421.1996.tb03338.x"
  ],
  "URL": "http://dx.doi.org/10.1111/j.1348-0421.1996.tb03338.x",
  "relation": {},
  "ISSN": [
    "0385-5600",
    "1348-0421"
  ],
  "subject": [],
  "container-title-short": "Microbiology and Immunology",
  "published": {
    "date-parts": [
      [
        1996,
        3
      ]
    ]
  }
}