DOI: 10.1111/j.1348-0421.1995.tb03271.x. Analysis of the Relationship between Cellular Thymidine Kinase Activity and Virulence of Thymidine Kinase‐Negative Herpes Simplex Virus Types 1 and 2

Analysis of the Relationship between Cellular Thymidine Kinase Activity and Virulence of Thymidine Kinase‐Negative Herpes Simplex Virus Types 1 and 2

10.1111/j.1348-0421.1995.tb03271.x

Crossref journal-article

Wiley

Microbiology and Immunology (311)

Abstract

AbstractThe virulence of thymidine kinase‐negative herpes simplex virus type 1 (HSV‐1; VRTK− strain) and type 2 (HSV‐2; UWTK− strain) was studied in comparison with that of their parental strains (VR‐3 and UW‐268, respectively) in an encephalitis model of adult (4‐week‐old) and newborn (3‐day‐old) mice. Viral thymidine kinase (TK) activity was essential for the maximum expression of virulence of HSV‐1, because the 50% lethal dose (LD50) of VRTK− was 60 times higher than that of VR‐3 in the brains of newborn mice expressing high levels of cellular TK activity. However, the UWTK− strain showed the same virulence as the parental strain in newborn mice, despite the lack virulence in adults, suggesting that replication of the UWTK− strain was completely supported by cellular TK activity. This difference in the role of viral and cellular TKs for virus growth between HSV‐1 and HSV‐2 was confirmed with the one‐step growth of virus strains in L‐M and L‐M(TK−) cells.

Bibliography

Suzutani, T., Koyano, S., Takada, M., Yoshida, I., & Azuma, M. (1995). Analysis of the Relationship between Cellular Thymidine Kinase Activity and Virulence of Thymidine KinaseâNegative Herpes Simplex Virus Types 1 and 2. Microbiology and Immunology, 39(10), 787â794. Portico.

Authors 5

Tatsuo Suzutani (first)
Shin Koyano (additional)
Minoru Takada (additional)
Itsuro Yoshida (additional)
Masanobu Azuma (additional)

References 34 Referenced 22

10.1007/BF01311326
10.1099/0022-1317-72-9-2043
10.1016/S0140-6736(82)91620-8
{'key': 'e_1_2_2_5_1', 'first-page': '494', 'article-title': 'Point mutation analysis in a mammalian gene: rapid preparation of total RNA, PCR amplification of cDNA, and Taq sequencing by a novel method', 'volume': '7', 'author': 'Carothers A.M.', 'year': '1989', 'journal-title': 'Biotechniques'} / Biotechniques / Point mutation analysis in a mammalian gene: rapid preparation of total RNA, PCR amplification of cDNA, and Taq sequencing by a novel method by Carothers A.M. (1989)
10.1016/0042-6822(91)90093-Q
10.1128/AAC.36.8.1589
10.1073/pnas.89.8.3266
10.1073/pnas.86.12.4736
10.1099/0022-1317-70-4-869
10.1128/AAC.31.7.1117
10.7326/0003-4819-76-3-112-6-416
10.1017/S0022172400025109
10.1099/0022-1317-24-3-465
10.1016/0167-4781(83)90056-8
10.1128/AAC.31.10.1483
10.1128/jvi.63.11.4976-4978.1989 / J. Virol. / Latent infections in spinal ganglia with thymidine kinase‐deficient herpes simplex virus by Leist T.P. (1989)
10.1099/0022-1317-69-7-1531
10.1128/AAC.28.6.740
10.1128/JVI.65.8.4520-4524.1991
10.1016/S0140-6736(87)91157-3
10.7326/0003-4819-111-11-893
10.1016/S0140-6736(84)92623-0
10.1128/AAC.32.7.1046
10.1128/AAC.32.10.1547
10.1128/jvi.66.4.2118-2124.1992 / J. Virol. / Random mutagenesis of the thymidine kinase gene of varicella‐zoster virus by Suzutani T. (1992)
10.1099/0022-1317-70-3-705
10.1126/science.224454
10.1128/jcm.17.1.122-127.1983 / J. Clin. Microbiol. / Thymidine plaque autoradiography of thymidine kinase‐positive and thymidine kinase‐negative herpesviruses by Tenser R.B. (1983)
10.1093/infdis/147.5.956
10.1128/jvi.63.6.2861-2865.1989 / J. Virol. / Latency‐associated transcript but not reactivatable virus is present in sensory ganglion neurons after inoculation of thymidine kinase‐negative mutants of herpes simplex virus type 1 by Tenser R.B. (1989)
10.1093/infdis/148.6.1077
10.1073/pnas.78.3.1441
10.1056/NEJM198601163140303
10.1056/NEJM199102143240703

Dates

Type	When
Created	11 years, 9 months ago (Nov. 14, 2013, 12:14 p.m.)
Deposited	1 year, 9 months ago (Nov. 20, 2023, 5:28 p.m.)
Indexed	1 year, 9 months ago (Nov. 20, 2023, 7:07 p.m.)
Issued	29 years, 10 months ago (Oct. 1, 1995)
Published	29 years, 10 months ago (Oct. 1, 1995)
Published Online	11 years, 9 months ago (Nov. 14, 2013)
Published Print	29 years, 10 months ago (Oct. 1, 1995)

Funders 0

None

BibTeX

@article{Suzutani_1995, title={Analysis of the Relationship between Cellular Thymidine Kinase Activity and Virulence of Thymidine Kinase‐Negative Herpes Simplex Virus Types 1 and 2}, volume={39}, ISSN={1348-0421}, url={http://dx.doi.org/10.1111/j.1348-0421.1995.tb03271.x}, DOI={10.1111/j.1348-0421.1995.tb03271.x}, number={10}, journal={Microbiology and Immunology}, publisher={Wiley}, author={Suzutani, Tatsuo and Koyano, Shin and Takada, Minoru and Yoshida, Itsuro and Azuma, Masanobu}, year={1995}, month=oct, pages={787–794} }

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